Pseudo-outbreak of Bacillaceae species associated with poor compliance with blood culture collection recommendations.

IF 3.7 3区 医学 Q2 INFECTIOUS DISEASES European Journal of Clinical Microbiology & Infectious Diseases Pub Date : 2024-12-01 Epub Date: 2024-09-27 DOI:10.1007/s10096-024-04925-5
Alina Maria Borcan, Carmen-Cristina Vasile, Alina-Ioana Popa, Cristina Andreea Badea, Gabriel Adrian Popescu, Daniela Tălăpan
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Abstract

Purpose: This study describes a pseudo-outbreak of Bacillaceae spp. bloodstream infections that spanned five months starting in May 2023 and the infection prevention measures implemented to control it.

Methods: This retrospective study was conducted at a tertiary infectious disease hospital in Bucharest, Romania. An observational audit of the blood culture collection practice in our hospital was performed, and the materials used during blood culture collection were sampled. Bacterial colonies were identified using MALDI Biotyper. The Bacillaceae blood culture positivity rates in the previous four years were compared using the Kruskal‒Wallis rank test.

Results: Bacillaceae spp.-positive blood cultures were recovered from 60 patients over a five-month period. In the case of 58 patients, Bacillaceae spp.-positive blood cultures were considered contaminated. Two patients were treated for Bacillus spp. bacteraemia. The audit revealed significant variation during the preparation of the venipuncture site step and the use of nonsterile medical cotton wool. Medical cotton wool contaminated with species of Bacillaceae was found in 10 out of 12 wards. The control measures included repeated training on the blood culture collection procedure and the removal of Bacillaceae spp.-contaminated cotton wool.

Conclusions: The pseudo-outbreak was caused by the unjustified use of medical cotton wool for disinfection of the skin and blood culture bottle septums. The investigation of this pseudo-outbreak highlighted a gap in blood culture collection practices at our facility and thus allowed for its improvement.

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假性芽孢杆菌属疫情爆发与未严格遵守血液培养物采集建议有关。
目的:本研究描述了自 2023 年 5 月起持续 5 个月的假性芽孢杆菌属血流感染疫情,以及为控制疫情而采取的感染预防措施:这项回顾性研究在罗马尼亚布加勒斯特的一家三级传染病医院进行。我们对医院的血培养采集工作进行了观察审计,并对血培养采集过程中使用的材料进行了采样。使用 MALDI 生物分析仪对细菌菌落进行鉴定。采用 Kruskal-Wallis 秩检验比较了过去四年中巴氏杆菌科细菌血培养阳性率:结果:在五个月的时间里,60 名患者的血液培养出了阳性芽孢杆菌科细菌。其中有 58 例患者的血液培养物为枯草杆菌属阳性,被认为是受到了污染。两名患者因杆菌属菌血症接受了治疗。审计结果显示,在静脉穿刺部位的准备步骤和使用未经消毒的医用脱脂棉方面存在很大差异。在 12 间病房中,有 10 间发现医用脱脂棉受到芽孢杆菌污染。控制措施包括反复培训血液培养采集程序和清除受巴氏杆菌污染的药棉:假性疫情爆发的原因是不合理使用医用脱脂棉消毒皮肤和血培养瓶隔膜。对这起假性疫情的调查凸显了我院在血培养采集操作方面存在的不足,并因此得以改进。
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来源期刊
CiteScore
10.40
自引率
2.20%
发文量
138
审稿时长
1 months
期刊介绍: EJCMID is an interdisciplinary journal devoted to the publication of communications on infectious diseases of bacterial, viral and parasitic origin.
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