Identification of an active fraction of Kangfuxin in the treatment of periodontitis in a rat model.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2024-01-01 Epub Date: 2024-09-27 DOI:10.5603/fhc.101230

Yanli Tang, Jie Pan, Hui Guo, Qiyan Li

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Abstract

Introduction: Periodontitis is a serious gum infection that disrupts the soft tissue around teeth. This study aimed to identify the most effective fraction of the Chinese medicine Kangfuxin for periodontitis treatment in a rat model.

Material and methods: Kangfuxin solution was subjected to sequential extraction using chloroform, ethyl acetate, n-butanol, and water. The extracts were evaporated, dissolved in DMSO, diluted in water, and administered to rats via gavage (0.5 mL/day) for 2 weeks. The n-butanol extract was further fractionated using macroporous resin chromatography with 10%, 30%, 50%, 70%, and 90% ethanol elution. Levels of inflammatory cytokines IL-6, IL-1β, and TNF-α in periodontitis samples were examined by ELISA. Leukocyte infiltration in the cementum was analysed by haematoxylin and eosin (H&E) staining.

Results: The n-butanol extract showed the best anti-inflammatory effect, reducing IL-6, IL-1β, and TNF-α levels in periodontitis samples and alleviating tissue damage and leukocyte infiltration in the cementum. Further fractionation revealed that the 50% ethanol fraction of the n-butanol extract had the most potent action in attenuating inflammation. This fraction suppressed the activation of the PI3K-AKT-mTOR signalling pathway in periodontitis samples. Application of a PI3K activator counteracted the anti-inflammatory effect of the 50% ethanol fraction.

Conclusions: We identified a potent anti-inflammatory fraction (50% ethanol fraction of the n-butanol extract) of Kangfuxin for periodontitis treatment. This fraction suppressed the activity of the PI3K-AKT-mTOR signalling pathway in periodontitis samples. Further research is needed to isolate and characterise the specific bioactive compounds within this fraction.

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鉴定康复欣在大鼠牙周炎治疗模型中的活性成分。

简介牙周炎是一种严重的牙龈感染，会破坏牙齿周围的软组织。本研究旨在确定中药康福欣在大鼠模型中治疗牙周炎的最有效组分：康复欣溶液用氯仿、乙酸乙酯、正丁醇和水依次提取。蒸发提取物，将其溶于二甲基亚砜（DMSO）中，用水稀释后给大鼠灌胃（每天 0.5 毫升），连续两周。正丁醇提取物使用大孔树脂色谱法进一步分馏，乙醇洗脱率分别为 10%、30%、50%、70% 和 90%。通过 ELISA 检测牙周炎样本中炎症细胞因子 IL-6、IL-1β 和 TNF-α 的水平。用苏木精和伊红（H&E）染色法分析牙槽骨中的白细胞浸润情况：结果：正丁醇提取物的抗炎效果最好，它能降低牙周炎样本中的 IL-6、IL-1β 和 TNF-α 水平，减轻组织损伤和牙槽骨中的白细胞浸润。进一步的分馏结果表明，正丁醇提取物的 50%乙醇馏分具有最有效的消炎作用。该馏分抑制了牙周炎样本中 PI3K-AKT-mTOR 信号通路的激活。使用 PI3K 激活剂可抵消 50% 乙醇馏分的抗炎作用：我们发现了一种治疗牙周炎的强效抗炎馏分（正丁醇提取物的 50%乙醇馏分）。该成分抑制了牙周炎样本中 PI3K-AKT-mTOR 信号通路的活性。还需要进一步的研究来分离和鉴定该馏分中的特定生物活性化合物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.

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