Evaluation of testing face-mask filter samples with LAMP shows high rates of detection in pulmonary TB.

IF 3.4 3区 医学 Q2 INFECTIOUS DISEASES International Journal of Tuberculosis and Lung Disease Pub Date : 2024-10-01 DOI:10.5588/ijtld.24.0190
T Kodama, K Chikamatsu, K Kamada, K Mizuno, Y Morishige, Y Igarashi, A Osugi, A Aono, Y Murase, M Okumura, T Yoshiyama, A Takaki, S Mitarai
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Abstract

BACKGROUNDDetection of Mycobacterium tuberculosis (MTB) in bioaerosols derived from patients with active pulmonary TB is a potential alternative diagnostic method for patients with presumed TB who cannot expectorate sputum.OBJECTIVETo assess the efficacy of a bioaerosol particle collection method to capture MTB and diagnose TB.METHODSA mask-like filter holder (3D mask) with a water-soluble gelatine filter (GF) and one containing a water-insoluble polypropylene filter (PPF) were prepared. Eligible patients wore the 3D mask with GF or PPF within 3 days of starting anti-TB drugs. The GF and PPF filters were collected after 2 and 8 h. DNA was extracted from the filter samples and tested using loop-mediated isothermal amplification (LAMP).RESULTSFilter samples were collected from 57 and 20 patients with and without active pulmonary TB, respectively. The GF and PPF sensitivity was 76.2% and 83.3%, respectively. The specificity of both methods was 100%. Of the 57 patients diagnosed with non-expectorated sputum samples, including suction phlegm, gastric lavage, and bronchial lavage fluid, 55.6% and 50.0% were positive by GF and PPF, respectively.CONCLUSIONWe present a 3D mask filter sampling method for exhaled bioaerosol particles that can be used in clinical practice to diagnose patients with presumed TB..

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用 LAMP 检测面罩过滤器样本的评估显示,肺结核的检出率很高。
背景从活动性肺结核患者产生的生物气溶胶中检测结核分枝杆菌(MTB)是一种潜在的替代诊断方法,适用于无法排痰的推测肺结核患者。符合条件的患者在开始服用抗结核药物后 3 天内佩戴装有 GF 或 PPF 的三维口罩。结果分别从 57 名和 20 名活动性肺结核患者和非活动性肺结核患者身上采集了过滤器样本。GF 和 PPF 的灵敏度分别为 76.2% 和 83.3%。两种方法的特异性均为 100%。在 57 名诊断为非排痰(包括吸痰、洗胃和支气管灌洗液)的患者中,GF 和 PPF 的阳性率分别为 55.6% 和 50.0%。
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来源期刊
CiteScore
4.90
自引率
20.00%
发文量
266
审稿时长
2 months
期刊介绍: The International Journal of Tuberculosis and Lung Disease publishes articles on all aspects of lung health, including public health-related issues such as training programmes, cost-benefit analysis, legislation, epidemiology, intervention studies and health systems research. The IJTLD is dedicated to the continuing education of physicians and health personnel and the dissemination of information on tuberculosis and lung health world-wide.
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