Electrospun Silk-ICG Composite Fibers and the Application toward Hemorrhage Control.

IF 5 3区 医学 Q1 ENGINEERING, BIOMEDICAL Journal of Functional Biomaterials Pub Date : 2024-09-19 DOI:10.3390/jfb15090272
Ayesha Siddiqua, Elwin Clutter, Olga Garklavs, Hemalatha Kanniyappan, Rong R Wang
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Abstract

In trauma and surgery, efficient hemorrhage control is crucial to avert fatal blood loss and increase the likelihood of survival. There is a significant demand for novel biomaterials capable of promptly and effectively managing bleeding. This study aimed to develop flexible biocomposite fibrous scaffolds with an electrospinning technique using silk fibroin (SF) and indocyanine green (ICG). The FDA-approved ICG dye has unique photothermal properties. The water permeability, degradability, and biocompatibility of Bombyx mori cocoon-derived SF make it promising for biomedical applications. While as-spun SF-ICG fibers were dissolvable in water, ethanol vapor treatment (EVT) effectively induced secondary structural changes to promote β-sheet formation. This resulted in significantly improved aqueous stability and mechanical strength of the fibers, thereby increasing their fluid uptake capability. The enhanced SF-ICG interaction effectively prevented ICG leaching from the composite fibers, enabling them to generate heat under NIR irradiation due to ICG's photothermal properties. Our results showed that an SF-ICG 0.4% fibrous matrix can uptake 473% water. When water was replaced by bovine blood, a 25 s NIR irradiation induced complete blood coagulation. However, pure silk did not have the same effect. Additionally, NIR irradiation of the SF-ICG fibers successfully stopped the flow of blood in an in vitro model that mimicked a damaged blood vessel. This novel breakthrough offers a biotextile platform poised to enhance patient outcomes across various medical scenarios, representing a significant milestone in functional biomaterials.

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电纺丝-ICG 复合纤维及其在止血方面的应用
在创伤和外科手术中,有效控制出血对于避免致命失血和提高存活率至关重要。对能够及时有效控制出血的新型生物材料的需求量很大。本研究旨在利用丝纤维蛋白(SF)和吲哚菁绿(ICG),通过电纺丝技术开发柔性生物复合纤维支架。美国 FDA 批准的 ICG 染料具有独特的光热特性。从蚕茧中提取的蚕丝纤维素具有透水性、可降解性和生物相容性,因此在生物医学应用中大有可为。虽然原纺丝 SF-ICG 纤维可溶于水,但乙醇蒸汽处理(EVT)可有效诱导二次结构变化,促进 β 片形成。这大大提高了纤维的水稳定性和机械强度,从而增强了其吸液能力。SF-ICG 相互作用的增强有效地防止了 ICG 从复合纤维中浸出,使其能够在近红外照射下利用 ICG 的光热特性产生热量。我们的研究结果表明,SF-ICG 含量为 0.4% 的纤维基质可吸收 473% 的水分。当用牛血代替水时,25 秒的近红外照射可诱导血液完全凝固。然而,纯丝却没有同样的效果。此外,在模拟受损血管的体外模型中,近红外照射 SF-ICG 纤维可成功阻止血液流动。这一新颖的突破提供了一个生物纺织平台,有望在各种医疗场景中提高病人的治疗效果,是功能性生物材料领域的一个重要里程碑。
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来源期刊
Journal of Functional Biomaterials
Journal of Functional Biomaterials Engineering-Biomedical Engineering
CiteScore
4.60
自引率
4.20%
发文量
226
审稿时长
11 weeks
期刊介绍: Journal of Functional Biomaterials (JFB, ISSN 2079-4983) is an international and interdisciplinary scientific journal that publishes regular research papers (articles), reviews and short communications about applications of materials for biomedical use. JFB covers subjects from chemistry, pharmacy, biology, physics over to engineering. The journal focuses on the preparation, performance and use of functional biomaterials in biomedical devices and their behaviour in physiological environments. Our aim is to encourage scientists to publish their results in as much detail as possible. Therefore, there is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Several topical special issues will be published. Scope: adhesion, adsorption, biocompatibility, biohybrid materials, bio-inert materials, biomaterials, biomedical devices, biomimetic materials, bone repair, cardiovascular devices, ceramics, composite materials, dental implants, dental materials, drug delivery systems, functional biopolymers, glasses, hyper branched polymers, molecularly imprinted polymers (MIPs), nanomedicine, nanoparticles, nanotechnology, natural materials, self-assembly smart materials, stimuli responsive materials, surface modification, tissue devices, tissue engineering, tissue-derived materials, urological devices.
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