Diosmetin Promotes Early Embryonic Development in Pigs by Alleviating Oxidative Stress

IF 2.7 3区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Molecular Reproduction and Development Pub Date : 2024-09-30 DOI:10.1002/mrd.23775

Jia-Jun Ren, Xiu-Wen Yuan, Zi-Long Meng, Neng-Hao Cao, Yong-Nan Xu, Nam-Hyung Kim, Ying-Hua Li

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Abstract

Diosmetin (DIOS), a natural flavonoid monomer derived from lemons and present in various plants such as spearmint and spider moss, exhibits antioxidant, anti-inflammatory, and antiaging properties. Nonetheless, its impact on early embryonic development in pigs remains unexplored. This study aimed to determine the influence of DIOS supplementation in an in vitro culture (IVC) medium on porcine embryo development and to elucidate the underlying mechanisms. Findings revealed that embryos cultured in IVC medium with 0.1 μM DIOS demonstrated an increased blastocyst formation rate, higher total cell number, reduced LC3B and CASPASE3 levels, elevated Nrf2 levels, decreased ROS, and enhanced GSH and mitochondrial membrane potential at the 4-cell embryonic stage. Additionally, the expression of proapoptotic genes (CAS3, CAS8, and BAX) and autophagy-related genes (BECLIN1, ATG5, LC3B, and P62) was downregulated, whereas the expression of embryonic development-related genes (CDK1 and CDK2), antioxidant-related genes (SOD1 and SOD2), and mitochondrial biogenesis-related genes (NRF2) was upregulated. These findings suggest that DIOS promotes early embryonic development in pigs by mitigating oxidative stress and enhancing mitochondrial function, thereby reducing autophagy and apoptosis levels.

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香叶木素通过缓解氧化应激促进猪的早期胚胎发育

香叶木素（DIOS）是从柠檬中提取的一种天然类黄酮单体，存在于多种植物（如留兰香和蜘蛛苔）中，具有抗氧化、抗炎和抗衰老的特性。然而，它对猪早期胚胎发育的影响仍有待探索。本研究旨在确定在体外培养（IVC）介质中补充 DIOS 对猪胚胎发育的影响，并阐明其潜在机制。研究结果显示，在含有 0.1 μM DIOS 的 IVC 培养基中培养的胚胎在 4 细胞胚胎阶段表现出更高的囊胚形成率、更高的细胞总数、更低的 LC3B 和 CASPASE3 水平、更高的 Nrf2 水平、更低的 ROS 以及更高的 GSH 和线粒体膜电位。此外，促凋亡基因（CAS3、CAS8 和 BAX）和自噬相关基因（BECLIN1、ATG5、LC3B 和 P62）的表达下调，而胚胎发育相关基因（CDK1 和 CDK2）、抗氧化相关基因（SOD1 和 SOD2）和线粒体生物生成相关基因（NRF2）的表达上调。这些研究结果表明，DIOS 可通过减轻氧化应激和增强线粒体功能促进猪的早期胚胎发育，从而降低自噬和细胞凋亡水平。

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来源期刊

Molecular Reproduction and Development 生物-发育生物学

CiteScore

5.20

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Molecular Reproduction and Development takes an integrated, systems-biology approach to understand the dynamic continuum of cellular, reproductive, and developmental processes. This journal fosters dialogue among diverse disciplines through primary research communications and educational forums, with the philosophy that fundamental findings within the life sciences result from a convergence of disciplines. Increasingly, readers of the Journal need to be informed of diverse, yet integrated, topics impinging on their areas of interest. This requires an expansion in thinking towards non-traditional, interdisciplinary experimental design and data analysis.