Study on the cross-resistance of Aedes albopictus (Skuse) (Diptera: Culicidae) to deltamethrin and pyriproxyfen.

IF 3 2区医学 Q1 PARASITOLOGY Parasites & Vectors Pub Date : 2024-09-27 DOI:10.1186/s13071-024-06485-1

Ling-Qun Lin, Ya-Hui Chen, Yi-Fan Tian, Yu-Sen Chen, Zhao-Yang Zheng, Jing-Xin Wu, Fen Hu, Cheng Wu, Li-Hua Xie

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Abstract

Background: Insecticide resistance poses a significant challenge in the implementation of vector-borne disease control strategies. We have assessed the resistance levels of Aedes albopictus to deltamethrin and pyriproxyfen (PPF) in Fujian Province (China) and investigated the correlation between these resistance levels and mutations in the voltage-gated sodium channel (VGSC).

Methods: The WHO bioassay protocol was used to evaluate the resistance coefficient of Ae. albopictus to deltamethrin and PPF, comparing a susceptible population from the Foshan (FS) area with wild populations from the Sanming (SM), Quanzhou (QZ), Zhangzhou (ZZ), Putian (PT) and Fuzhou (FZ) areas in Fujian Province. Genomic DNA was analyzed by PCR and sequencing to detect knockdown resistance (kdr) in the VGSC, specifically at the pyrethroid resistance alleles V1016V, I1532I and F1534F. Molecular docking was also performed to analyze the binding interactions of PPF and its metabolite 4'-OH-PPF to cytochrome P450 (CYP) 2C19, 2C9 and 3A4 and Ae. albopictus methoprene-tolerant receptors (AeMet), respectively.

Results: The analysis of resistance to deltamethrin and PPF among Ae. albopictus populations from the various regions revealed that except for the sensitive population in FS and the SM population, the remaining four regional populations demonstrated resistance levels ranging from 4.31- to 18.87-fold for deltamethrin and from 2.85- to 3.62-fold for PPF. Specifically, the FZ and PT populations exhibited high resistance to deltamethrin, whereas the ZZ and QZ populations approached moderate resistance levels. Also, the resistance of the FZ, PT and ZZ populations to PPF increased slowly but consistently with the increasing trend of deltamethrin resistance. Genomic analysis identified multiple non-synonymous mutations within the VGSC gene; the F1534S and F1534L mutations showed significant resistance to deltamethrin in Ae. albopictus. Molecular docking results revealed that PPF and its metabolite 4'-OH-PPF bind to the Ae. albopictus AeMet receptor and CYP2C19.

Conclusions: The wild Ae. albopictus populations of Fujian Province showed varying degrees of resistance to deltamethrin and PPF and a trend of cross-resistance to deltamethrin and PPF. Increased vigilance is needed for potential higher levels of cross-resistance, especially in the PT and FZ regions.

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白纹伊蚊（Skuse）（双翅目：库蚊科）对溴氰菊酯和吡丙醚的交叉抗药性研究。

背景：杀虫剂抗药性是实施病媒传播疾病控制战略的重大挑战。我们评估了福建省白纹伊蚊对溴氰菊酯和吡丙醚（PPF）的抗药性水平，并研究了这些抗药性水平与电压门控钠通道（VGSC）突变之间的相关性：方法：采用WHO生物测定方案评估白纹伊蚊对溴氰菊酯和PPF的抗性系数，将佛山（FS）地区的易感种群与福建省三明（SM）、泉州（QZ）、漳州（ZZ）、莆田（PT）和福州（FZ）地区的野生种群进行比较。通过 PCR 和测序分析基因组 DNA，检测 VGSC 的抗性基因敲除（kdr），特别是拟除虫菊酯抗性等位基因 V1016V、I1532I 和 F1534F。还进行了分子对接，以分析 PPF 及其代谢物 4'-OH-PPF 分别与细胞色素 P450（CYP）2C19、2C9 和 3A4 以及白纹伊蚊耐甲氧苄啶受体（AeMet）的结合相互作用：对各地区白纹伊蚊种群对溴氰菊酯和 PPF 的抗性分析表明，除 FS 和 SM 的敏感种群外，其余四个地区的种群对溴氰菊酯的抗性水平从 4.31 倍到 18.87 倍不等，对 PPF 的抗性水平从 2.85 倍到 3.62 倍不等。具体而言，FZ 和 PT 群体对溴氰菊酯表现出较高的抗性，而 ZZ 和 QZ 群体则接近中等抗性水平。此外，FZ、PT 和 ZZ 群体对 PPF 的抗性增长缓慢，但与溴氰菊酯抗性的增长趋势一致。基因组分析发现了 VGSC 基因中的多个非同义突变；F1534S 和 F1534L 突变表明白纹伊蚊对溴氰菊酯具有显著抗性。分子对接结果显示，PPF 及其代谢物 4'-OH-PPF 与白纹伊蚊的 AeMet 受体和 CYP2C19 结合：结论：福建省野生白纹伊蚊种群对溴氰菊酯和PPF表现出不同程度的抗性，并有对溴氰菊酯和PPF交叉抗性的趋势。需要对潜在的更高水平的交叉抗性提高警惕，特别是在 PT 和 FZ 地区。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Parasites & Vectors 医学-寄生虫学

CiteScore

6.30

自引率

9.40%

发文量

433

审稿时长

1.4 months

期刊介绍： Parasites & Vectors is an open access, peer-reviewed online journal dealing with the biology of parasites, parasitic diseases, intermediate hosts, vectors and vector-borne pathogens. Manuscripts published in this journal will be available to all worldwide, with no barriers to access, immediately following acceptance. However, authors retain the copyright of their material and may use it, or distribute it, as they wish. Manuscripts on all aspects of the basic and applied biology of parasites, intermediate hosts, vectors and vector-borne pathogens will be considered. In addition to the traditional and well-established areas of science in these fields, we also aim to provide a vehicle for publication of the rapidly developing resources and technology in parasite, intermediate host and vector genomics and their impacts on biological research. We are able to publish large datasets and extensive results, frequently associated with genomic and post-genomic technologies, which are not readily accommodated in traditional journals. Manuscripts addressing broader issues, for example economics, social sciences and global climate change in relation to parasites, vectors and disease control, are also welcomed.