Anti-inflammatory effects of Tao Hong Si Wu Tang in mice with lung cancer and chronic obstructive pulmonary disease.

IF 2.6 Q3 ONCOLOGY World journal of clinical oncology Pub Date : 2024-09-24 DOI:10.5306/wjco.v15.i9.1198

Guo-Li Wang, Yan-Ling Xu, Ke-Ming Zhao, Ai-Feng Sui, Li-Na Wang, Hu Deng, Ge Wang

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Abstract

Background: Lung cancer (LC) combined with chronic obstructive pulmonary disease (COPD) is a common combination of comorbidities. Anti-inflammation and modulation of oxidative/antioxidative imbalance may prevent COPD-induced LC, and are also crucial to the treatment of LC combined with COPD. Modern studies have shown that Tao Hong Si Wu Tang (THSW) has vasodilatory, anti-inflammatory, anti-fatigue, anti-shock, immunoregulatory, lipid-reducing, micronutrient-supplementing, and anti-allergy effects.

Aim: To observe the effects of THSW on COPD and LC in mice.

Methods: A total of 100 specific pathogen-free C57/BL6 mice were randomly divided into five groups: Blank control group (group A), model control group (group B), THSW group (group C), IL-6 group (group D), and THSW + IL-6 group (group E), with 20 mice in each group. A COPD mouse model was established using fumigation plus lipopolysaccharide intra-airway drip, and an LC model was replicated by in situ inoculation using the Lewis cell method.

Results: The blank control group exhibited a clear alveolar structure. The model control and IL-6 groups had thickened alveolar walls, with smaller alveolar lumens, interstitial edema, and several inflammatory infiltrating cells. Histopathological changes in the lungs of the THSW and THSW + IL-6 groups were less than those of the model control group. The serum IL-1β, IL-6, and TNF-α levels and IL-6R, JAK, p-JAK, STAT1/3, p-STAT1/3, FOXO, p-FOXO, and IL-7R expression levels in lung tissues of mice in the rest of the groups were significantly higher than those of the blank control group (P < 0.01). Compared with the model control group, the IL-6 group demonstrated significantly higher levels for the abovementioned proteins in the serum and lung tissues (P < 0.01), and the THSW group had significantly higher serum IL-1β, IL-6, and TNF-α levels and IL-7R expression levels in lung tissues (P < 0.01) but significantly decreased IL-6R, JAK, p-JAK, STAT1/3, p-STAT1/3, FOXO, p-FOXO, and IL-7R levels (P < 0.01).

Conclusion: THSW reduces the serum IL-1β, IL-6, and TNF-α levels in the mouse model with anti-inflammatory effects. Its anti-inflammatory mechanism lies in inhibiting the overactivation of the JAK/STAT1/3 signaling pathway.

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桃红四物汤对肺癌和慢性阻塞性肺病小鼠的抗炎作用

背景：肺癌（LC）合并慢性阻塞性肺疾病（COPD）是一种常见的合并症。抗炎和调节氧化/抗氧化失衡可预防慢性阻塞性肺病引起的肺癌，也是治疗肺癌合并慢性阻塞性肺病的关键。现代研究表明，桃红四物汤具有扩张血管、抗炎、抗疲劳、抗休克、免疫调节、降脂、补充微量元素和抗过敏等作用：方法：将 100 只无特定病原体的 C57/BL6 小鼠随机分为五组：空白对照组（A 组）、模型对照组（B 组）、THSW 组（C 组）、IL-6 组（D 组）和 THSW + IL-6 组（E 组），每组 20 只。采用熏蒸加脂多糖气道内滴注法建立了 COPD 小鼠模型，并采用 Lewis 细胞法原位接种复制了 LC 模型：结果：空白对照组的肺泡结构清晰。结果：空白对照组肺泡结构清晰，模型对照组和 IL-6 组肺泡壁增厚，肺泡腔变小，肺间质水肿，并有多个炎性浸润细胞。THSW 组和 THSW + IL-6 组的肺组织病理学变化小于模型对照组。其余各组小鼠肺组织中血清IL-1β、IL-6和TNF-α水平及IL-6R、JAK、p-JAK、STAT1/3、p-STAT1/3、FOXO、p-FOXO和IL-7R表达水平均显著高于空白对照组（P＜0.01）。与模型对照组相比，IL-6组小鼠血清和肺组织中的上述蛋白水平明显升高（P＜0.01），THSW组小鼠血清中IL-1β、IL-6和TNF-α水平及肺组织中IL-7R表达水平明显升高（P＜0.01），但IL-6R、JAK、p-JAK、STAT1/3、p-STAT1/3、FOXO、p-FOXO和IL-7R水平明显降低（P＜0.01）：THSW 可降低小鼠模型的血清 IL-1β、IL-6 和 TNF-α 水平，具有抗炎作用。其抗炎机制在于抑制 JAK/STAT1/3 信号通路的过度激活。

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来源期刊

World journal of clinical oncology ONCOLOGY-

自引率

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发文量

585

期刊介绍： The WJCO is a high-quality, peer reviewed, open-access journal. The primary task of WJCO is to rapidly publish high-quality original articles, reviews, editorials, and case reports in the field of oncology. In order to promote productive academic communication, the peer review process for the WJCO is transparent; to this end, all published manuscripts are accompanied by the anonymized reviewers’ comments as well as the authors’ responses. The primary aims of the WJCO are to improve diagnostic, therapeutic and preventive modalities and the skills of clinicians and to guide clinical practice in oncology. Scope: Art of Oncology, Biology of Neoplasia, Breast Cancer, Cancer Prevention and Control, Cancer-Related Complications, Diagnosis in Oncology, Gastrointestinal Cancer, Genetic Testing For Cancer, Gynecologic Cancer, Head and Neck Cancer, Hematologic Malignancy, Lung Cancer, Melanoma, Molecular Oncology, Neurooncology, Palliative and Supportive Care, Pediatric Oncology, Surgical Oncology, Translational Oncology, and Urologic Oncology.