Assessment of the toxic influence of locally formulated pesticides on hepatic and renal biomarkers in male Wistar rats.

IF 2.2 4区 医学 Q3 TOXICOLOGY Toxicology Research Pub Date : 2024-09-30 eCollection Date: 2024-10-01 DOI:10.1093/toxres/tfae157
Esther Itunuoluwa Adeyele, Esther Olutomilayo Ayanyemi, Rufus Ojo Akomolafe, Olaoluwa Olukiran Sesan, Omolara Titilayo Aladesanmi, Aderonke Okoya Adetutu
{"title":"Assessment of the toxic influence of locally formulated pesticides on hepatic and renal biomarkers in male Wistar rats.","authors":"Esther Itunuoluwa Adeyele, Esther Olutomilayo Ayanyemi, Rufus Ojo Akomolafe, Olaoluwa Olukiran Sesan, Omolara Titilayo Aladesanmi, Aderonke Okoya Adetutu","doi":"10.1093/toxres/tfae157","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>There is growing concern of the potential damage to vital organs after long term exposure to locally formulated pesticides in rural area of Nigeria. This study was designed to assessed the effects of the individual chemical compound and their combination on the kidney and liver of rats' model.</p><p><strong>Methodology: </strong>Fifty-four rats divided into six groups and three sub-groups were exposed to 25, 50 and 75% dose of each of the pesticide's LD<sub>50</sub> for 4 h at 3 days interval in an inhalation chamber for 28 days. Alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total bilirubin (TOT_BIL), creatinine and urea assay showed significant increase at the aforementioned doses in comparison to the control group. The red blood cell counts, hematocrit and hemoglobin concentrations were significantly altered in the rats administered varying doses of the pesticides when compared with the control. Similar result was obtained for the differential white blood cell counts. Histopathological examinations of the liver tissue of rats showed infiltrated sinusoids, traces of karypyknosis, vacuolar degeneration and microvesicular steatosis while that of the renal tissue showed glomeruli atrophy leading to widened Bowman's spaces as well as few shrunken glomeruli and varied level of degenerative tubular changes to tubular necrosis.</p><p><strong>Conclusion: </strong>This study established that individual pesticides and their mixture is toxic to the liver and kidney, as evidenced by the elevated markers of renal and liver functions and distortion of the structure of both organs as revealed by their photomicrographs. Therefore, it is a matter of public health significance to regularly monitor pesticide residues in foods and humans in order to assess the food safety risk and population exposure to pesticides.</p>","PeriodicalId":105,"journal":{"name":"Toxicology Research","volume":null,"pages":null},"PeriodicalIF":2.2000,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11442146/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Toxicology Research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1093/toxres/tfae157","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/10/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"TOXICOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Background: There is growing concern of the potential damage to vital organs after long term exposure to locally formulated pesticides in rural area of Nigeria. This study was designed to assessed the effects of the individual chemical compound and their combination on the kidney and liver of rats' model.

Methodology: Fifty-four rats divided into six groups and three sub-groups were exposed to 25, 50 and 75% dose of each of the pesticide's LD50 for 4 h at 3 days interval in an inhalation chamber for 28 days. Alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total bilirubin (TOT_BIL), creatinine and urea assay showed significant increase at the aforementioned doses in comparison to the control group. The red blood cell counts, hematocrit and hemoglobin concentrations were significantly altered in the rats administered varying doses of the pesticides when compared with the control. Similar result was obtained for the differential white blood cell counts. Histopathological examinations of the liver tissue of rats showed infiltrated sinusoids, traces of karypyknosis, vacuolar degeneration and microvesicular steatosis while that of the renal tissue showed glomeruli atrophy leading to widened Bowman's spaces as well as few shrunken glomeruli and varied level of degenerative tubular changes to tubular necrosis.

Conclusion: This study established that individual pesticides and their mixture is toxic to the liver and kidney, as evidenced by the elevated markers of renal and liver functions and distortion of the structure of both organs as revealed by their photomicrographs. Therefore, it is a matter of public health significance to regularly monitor pesticide residues in foods and humans in order to assess the food safety risk and population exposure to pesticides.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
评估本地配制的杀虫剂对雄性 Wistar 大鼠肝脏和肾脏生物标志物的毒性影响。
背景:在尼日利亚农村地区,人们越来越担心长期接触当地配制的杀虫剂会对重要器官造成潜在损害。本研究旨在评估单个化合物及其组合对大鼠肝肾的影响:将 54 只大鼠分为 6 组和 3 个亚组,在吸入室中分别暴露于 25%、50% 和 75% LD50 剂量的杀虫剂中,每组暴露 4 小时,间隔 3 天,共 28 天。与对照组相比,上述剂量下的丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、碱性磷酸酶(ALP)、总胆红素(TOT_BIL)、肌酐和尿素检测结果均显著增加。与对照组相比,施用不同剂量杀虫剂的大鼠的红细胞计数、血细胞比容和血红蛋白浓度都发生了显著变化。差异白细胞计数也有类似结果。大鼠肝脏组织的组织病理学检查结果显示,肝窦浸润、钙化、空泡变性和微囊脂肪变性;肾脏组织的组织病理学检查结果显示,肾小球萎缩导致鲍曼氏间隙增宽,少数肾小球萎缩,肾小管发生不同程度的退行性变化,甚至肾小管坏死:这项研究证实,单个农药及其混合物对肝脏和肾脏具有毒性,肝肾功能指标的升高和显微照片显示的肝肾器官结构的扭曲就是证明。因此,定期监测食品和人体中的农药残留量,以评估食品安全风险和人群农药摄入量,对公共卫生具有重要意义。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Toxicology Research
Toxicology Research TOXICOLOGY-
CiteScore
3.60
自引率
0.00%
发文量
82
期刊介绍: A multi-disciplinary journal covering the best research in both fundamental and applied aspects of toxicology
期刊最新文献
Design and computational analysis of a novel Azurin-BR2 chimeric protein against breast cancer. Synergistic effect of curcumin and Piperine loaded Niosomal nanoparticles on acute pulmonary toxicity induced by Paraquat in mice. Upregulation of ACSL, ND75, Vha26 and sesB genes by antiepileptic drugs resulted in genotoxicity in drosophila. Potential protective role of chlorogenic acid against cyclophosphamide-induced reproductive damage in male mice. The cliff-edge of toxicological concern: highlighting the potential issues of an over-reliance on "less-than-lifetime" thresholds.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1