Overcoming Bacteriophage Contamination in Bioprocessing: Strategies and Applications.

IF 10.7 2区 材料科学 Q1 CHEMISTRY, PHYSICAL Small Methods Pub Date : 2024-10-02 DOI:10.1002/smtd.202400932
Xuan Zou, Ziran Mo, Lianrong Wang, Shi Chen, Sang Yup Lee
{"title":"Overcoming Bacteriophage Contamination in Bioprocessing: Strategies and Applications.","authors":"Xuan Zou, Ziran Mo, Lianrong Wang, Shi Chen, Sang Yup Lee","doi":"10.1002/smtd.202400932","DOIUrl":null,"url":null,"abstract":"<p><p>Bacteriophage contamination has a devastating impact on the viability of bacterial hosts and can significantly reduce the productivity of bioprocesses in biotechnological industries. The consequences range from widespread fermentation failure to substantial economic losses, highlighting the urgent need for effective countermeasures. Conventional prevention methods, which focus primarily on the physical removal of bacteriophages from equipment, bioprocess units, and the environment, have proven ineffective in preventing phage entry and contamination. The coevolutionary dynamics between phages and their bacterial hosts have spurred the development of a diverse repertoire of antiviral defense mechanisms within microbial communities. These naturally occurring defense strategies can be harnessed through genetic engineering to convert phage-sensitive hosts into robust, phage-resistant cell factories, providing a strategic approach to mitigate the threats posed by bacteriophages to industrial bacterial processes. In this review, an overview of the various defense strategies and immune systems that curb the propagation of bacteriophages and highlight their applications in fermentation bioprocesses to combat phage contamination is provided. Additionally, the tactics employed by phages to circumvent these defense strategies are also discussed, as preventing the emergence of phage escape mutants is a key component of effective contamination management.</p>","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2400932"},"PeriodicalIF":10.7000,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Small Methods","FirstCategoryId":"88","ListUrlMain":"https://doi.org/10.1002/smtd.202400932","RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, PHYSICAL","Score":null,"Total":0}
引用次数: 0

Abstract

Bacteriophage contamination has a devastating impact on the viability of bacterial hosts and can significantly reduce the productivity of bioprocesses in biotechnological industries. The consequences range from widespread fermentation failure to substantial economic losses, highlighting the urgent need for effective countermeasures. Conventional prevention methods, which focus primarily on the physical removal of bacteriophages from equipment, bioprocess units, and the environment, have proven ineffective in preventing phage entry and contamination. The coevolutionary dynamics between phages and their bacterial hosts have spurred the development of a diverse repertoire of antiviral defense mechanisms within microbial communities. These naturally occurring defense strategies can be harnessed through genetic engineering to convert phage-sensitive hosts into robust, phage-resistant cell factories, providing a strategic approach to mitigate the threats posed by bacteriophages to industrial bacterial processes. In this review, an overview of the various defense strategies and immune systems that curb the propagation of bacteriophages and highlight their applications in fermentation bioprocesses to combat phage contamination is provided. Additionally, the tactics employed by phages to circumvent these defense strategies are also discussed, as preventing the emergence of phage escape mutants is a key component of effective contamination management.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
克服生物处理过程中的噬菌体污染:战略与应用》。
噬菌体污染对细菌宿主的生存能力具有破坏性影响,可大大降低生物技术产业中生物工艺的生产率。其后果从广泛的发酵失败到巨大的经济损失不等,突出表明迫切需要采取有效的应对措施。传统的预防方法主要侧重于从设备、生物处理装置和环境中物理清除噬菌体,但事实证明这些方法无法有效防止噬菌体的进入和污染。噬菌体与其细菌宿主之间的共同进化动态促进了微生物群落内多种抗病毒防御机制的发展。可以通过基因工程利用这些自然产生的防御策略,将对噬菌体敏感的宿主转化为强大的抗噬菌体细胞工厂,从而提供一种战略方法来减轻噬菌体对工业细菌过程造成的威胁。本综述概述了遏制噬菌体繁殖的各种防御策略和免疫系统,并重点介绍了它们在发酵生物工艺中对抗噬菌体污染的应用。此外,还讨论了噬菌体规避这些防御策略的策略,因为防止噬菌体逃逸突变体的出现是有效污染管理的关键组成部分。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Small Methods
Small Methods Materials Science-General Materials Science
CiteScore
17.40
自引率
1.60%
发文量
347
期刊介绍: Small Methods is a multidisciplinary journal that publishes groundbreaking research on methods relevant to nano- and microscale research. It welcomes contributions from the fields of materials science, biomedical science, chemistry, and physics, showcasing the latest advancements in experimental techniques. With a notable 2022 Impact Factor of 12.4 (Journal Citation Reports, Clarivate Analytics, 2023), Small Methods is recognized for its significant impact on the scientific community. The online ISSN for Small Methods is 2366-9608.
期刊最新文献
DNA-Assisted Separation of Nanoparticles. Fast Kinetics Enabled by Ion Enrichment Layer for Dendrite-Free Zinc Anode. Unraveling the Interplay Between Memristive and Magnetoresistive Behaviors in LaCoO3/SrTiO3 Superlattice-Based Neural Synaptic Devices. A Strategy for Mitigating Lattice Stress and Enhancing Cycle Stability Through Modulating Transition Metal Redox Sequence. Heterogeneity During the Formation of Waterborne Barrier Coating Revealed by Cryogenic Transmission Electron Microscopy.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1