Is there an association between salivary immune and microbial profile with dental health in systematically healthy children?

IF 3.1 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Clinical Oral Investigations Pub Date : 2024-10-03 DOI:10.1007/s00784-024-05969-9

Esti Davidovich, Hadar Sarne, Aviv Shmueli, David Polak

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Abstract

Objective: This study aimed to characterize the inflammatory profile of systemically healthy children's saliva and its association with clinical diagnoses of caries and gingival inflammation.

Materials and methods: Unstimulated saliva was collected from 100 children before clinical dental examinations. The saliva samples were analyzed for total protein and specific inflammatory cytokines (IL-10, IL-8, IL-6, and TNFα) with Bradford and ELISA assays, respectively. Salivary bacteria were quantified using a quantitative real-time polymerase chain assay. The salivary values were then correlated with age, DMFT index, plaque index (PI), and gingival index (GI).

Results: The mean age of the cohort was 8.08 ± 0.23 years with 49% females, the mean DMF of the cohort was 2.64 ± 0.31, the mean GI was 0.51 ± 0.06, and the mean PI was 1.33 ± 0.07. Significant correlations were found between PI with DMFT and GI. Children with DMFT > 2 had significantly higher levels of IL-8 compared with children with DMFT ≤ 2. IL-6 and TNFα were significantly higher among children with PI > 1 than among children with PI ≤ 1.

Conclusions: Salivary cytokine were found to be associate with clinical parameters as DMFT and PI, thus may be a potential tool that reflects dental health status.

Clinical relevance: The presence of salivary cytokines in children may reflect evaluation of dental caries and oral inflammation.

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唾液免疫和微生物特征与系统健康儿童的牙齿健康有关联吗？

研究目的本研究旨在描述全身健康儿童唾液的炎症特征及其与龋齿和牙龈炎症临床诊断的关联：在进行临床牙科检查之前，收集 100 名儿童的未受刺激唾液。用 Bradford 和 ELISA 方法分别分析了唾液样本中的总蛋白和特定炎症细胞因子（IL-10、IL-8、IL-6 和 TNFα）。唾液细菌用定量实时聚合酶链测定法进行量化。然后将唾液值与年龄、DMFT指数、牙菌斑指数（PI）和牙龈指数（GI）相关联：样本的平均年龄为（8.08 ± 0.23）岁，其中女性占 49%；样本的平均 DMF 为（2.64 ± 0.31）；平均 GI 为（0.51 ± 0.06）；平均 PI 为（1.33 ± 0.07）。PI与DMFT和GI之间存在显著相关性。与 DMFT ≤ 2 的儿童相比，DMFT > 2 的儿童 IL-8 水平明显更高。PI>1的儿童的IL-6和TNFα明显高于PI≤1的儿童：结论：研究发现唾液细胞因子与 DMFT 和 PI 等临床参数相关，因此唾液细胞因子可能是反映牙齿健康状况的潜在工具：临床意义：儿童唾液细胞因子的存在可反映龋齿和口腔炎症的评估情况。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical Oral Investigations 医学-牙科与口腔外科

CiteScore

6.30

自引率

5.90%

发文量

484

审稿时长

3 months

期刊介绍： The journal Clinical Oral Investigations is a multidisciplinary, international forum for publication of research from all fields of oral medicine. The journal publishes original scientific articles and invited reviews which provide up-to-date results of basic and clinical studies in oral and maxillofacial science and medicine. The aim is to clarify the relevance of new results to modern practice, for an international readership. Coverage includes maxillofacial and oral surgery, prosthetics and restorative dentistry, operative dentistry, endodontics, periodontology, orthodontics, dental materials science, clinical trials, epidemiology, pedodontics, oral implant, preventive dentistiry, oral pathology, oral basic sciences and more.