Untargeted Metabolomic Study of Patients with Wet Age-Related Macular Degeneration in Aqueous Humor.

IF 3.5 3区医学 Q2 GERIATRICS & GERONTOLOGY Clinical Interventions in Aging Pub Date : 2024-09-27 eCollection Date: 2024-01-01 DOI:10.2147/CIA.S475920

Qingquan Wei, Xianghong Tu, Qinghua Qiu, Li Wang

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Abstract

Purpose: The objective of this study was to ascertain metabolic biomarkers and investigate the metabolic alterations associated with aqueous humor (AH) in wet age-related macular degeneration (AMD).

Methods: AH samples were collected from a total of 20 participants, including 10 individuals diagnosed with wet AMD and 10 individuals undergoing cataract surgery, serving as the control group. Metabolomics analysis was performed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) to identify and quantify metabolites.

Results: A total of 155 metabolites were identified in the AH samples. Among them, 10 metabolites emerged as potential biomarkers capable of differentiating patients with wet AMD from the control group. In the AH of wet AMD patients, there was increased expression of Cardiolipin (CL) (72:5), Diglyceride (DG) (18:3_18:2), DG (36:5e) and Triglyceride (TG) (24:7), while the expression of Ceramides (Cer) (d32:0), Cer (d34:0), Cer (d36:0), Monogalactosyldiacylglycerol (MGDG) (16:1_18:3), Sphingosine (SPH) (d18:0) and TG (16:0_10:4_16:0) was down regulated.

Conclusion: Through metabolomics analysis of AH, this study successfully uncovered valuable metabolic biomarkers linked to wet AMD. These findings contribute to a more comprehensive understanding of the pathogenesis of wet AMD and offer potential avenues for the development of innovative treatment strategies for this condition.

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湿性老年性黄斑变性患者水液中的非靶向代谢组学研究

目的：本研究旨在确定代谢生物标志物，并调查与湿性老年性黄斑变性（AMD）相关的房水（AH）代谢改变：共收集了20名参与者的房水样本，其中包括10名确诊为湿性AMD的患者和10名接受白内障手术的对照组患者。采用液相色谱-串联质谱法（LC-MS/MS）进行代谢组学分析，以鉴定和量化代谢物：结果：AH样本中共鉴定出155种代谢物。结果：AH 样品中共鉴定出 155 种代谢物，其中有 10 种代谢物是能够区分湿性 AMD 患者和对照组的潜在生物标记物。在湿性 AMD 患者的 AH 中，心磷脂（CL）（72:5）、二甘油酯（DG）（18:3_18:2）、DG（36:5e）和甘油三酯（TG）（24：而神经酰胺（Cer）（d32:0）、神经酰胺（d34:0）、神经酰胺（d36:0）、单半乳糖二酰甘油（MGDG）（16:1_18:3）、鞘磷脂（SPH）（d18:0）和甘油三酯（TG）（16:0_10:4_16:0）的表达则受到调控。结论通过对 AH 进行代谢组学分析，本研究成功地发现了与湿性 AMD 相关的有价值的代谢生物标记物。这些发现有助于人们更全面地了解湿性老年黄斑变性的发病机制，并为开发针对这种疾病的创新治疗策略提供了潜在的途径。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical Interventions in Aging GERIATRICS & GERONTOLOGY-

CiteScore

6.80

自引率

2.80%

发文量

193

审稿时长

6-12 weeks

期刊介绍： Clinical Interventions in Aging, is an online, peer reviewed, open access journal focusing on concise rapid reporting of original research and reviews in aging. Special attention will be given to papers reporting on actual or potential clinical applications leading to improved prevention or treatment of disease or a greater understanding of pathological processes that result from maladaptive changes in the body associated with aging. This journal is directed at a wide array of scientists, engineers, pharmacists, pharmacologists and clinical specialists wishing to maintain an up to date knowledge of this exciting and emerging field.