Lorenzo Peruzzi, Jacopo Franzoni, Manuel Tiburtini, Emanuela Abidi, Emiliano Alù, Giulio Barone, Elisabetta Bianchi, Chiara Cataudella, Emanuela Di Iorio, Maria Guerrina, Fabio Mondello, Luca Paino, Mario Pentassuglia, Manuela Porrovecchio, Giovanni Rivieccio, Eugenia Siccardi, Adriano Stinca, Alessio Tei, Virginia Volanti, Antonio Giacò
{"title":"Different observers introduce not negligible biases in comparative karyomorphological studies.","authors":"Lorenzo Peruzzi, Jacopo Franzoni, Manuel Tiburtini, Emanuela Abidi, Emiliano Alù, Giulio Barone, Elisabetta Bianchi, Chiara Cataudella, Emanuela Di Iorio, Maria Guerrina, Fabio Mondello, Luca Paino, Mario Pentassuglia, Manuela Porrovecchio, Giovanni Rivieccio, Eugenia Siccardi, Adriano Stinca, Alessio Tei, Virginia Volanti, Antonio Giacò","doi":"10.3897/compcytogen.18.135172","DOIUrl":null,"url":null,"abstract":"<p><p>Within a practical course of cytotaxonomy organized in Pisa (Italy) on February 2024 by the Group for Floristics, Systematics and Evolution of the Italian Botanical Society, we tested whether using image analysis softwares possible biases are still introduced by different observers. We conclude that observer bias selectively applies in possibly overestimating the length of short arms in a karyotype. As a consequence, the parameters most sensitive to these possible errors are CV<sub>CI</sub> and CV<sub>CL</sub>, and to a less degree M<sub>CA</sub> and THL. To achieve more stable results among observers, a still lacking standardized measurement protocol could be helpful.</p>","PeriodicalId":50656,"journal":{"name":"Comparative Cytogenetics","volume":null,"pages":null},"PeriodicalIF":1.0000,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11445605/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Comparative Cytogenetics","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3897/compcytogen.18.135172","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q4","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
引用次数: 0
Abstract
Within a practical course of cytotaxonomy organized in Pisa (Italy) on February 2024 by the Group for Floristics, Systematics and Evolution of the Italian Botanical Society, we tested whether using image analysis softwares possible biases are still introduced by different observers. We conclude that observer bias selectively applies in possibly overestimating the length of short arms in a karyotype. As a consequence, the parameters most sensitive to these possible errors are CVCI and CVCL, and to a less degree MCA and THL. To achieve more stable results among observers, a still lacking standardized measurement protocol could be helpful.
期刊介绍：
Comparative Cytogenetics is a peer-reviewed, open-access, rapid online journal launched to accelerate research on all aspects of plant and animal cytogenetics, karyosystematics, and molecular systematics.
All published papers can be freely copied, downloaded, printed and distributed at no charge for the reader. Authors are thus encouraged to post the pdf files of published papers on their homepages or elsewhere to expedite distribution. There is no charge for color.
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