Monita Sharma, Erin Huber, Emma Arnesdotter, Holger P Behrsing, Adam Bettmann, David Brandwein, Samuel Constant, Rahul Date, Abhay Deshpande, Eric Fabian, Amit Gupta, Robert Gutierrez, Arno C Gutleb, Marie M Hargrove, Michael Hollings, Victoria Hutter, Annie M Jarabek, Yulia Kaluzhny, Robert Landsiedel, Lawrence Milchak, Robert A Moyer, Jessica R Murray, Kathryn Page, Manish Patel, Stephanie N Pearson, Elijah J Petersen, Emily Reinke, Nuria Roldan, Clive Roper, Jamie B Scaglione, Raja S Settivari, Andreas O Stucki, Sandra Verstraelen, Joanne L Wallace, Shaun McCullough, Amy J Clippinger
{"title":"Minimum information for reporting on the TEER (trans-epithelial/endothelial electrical resistance) assay (MIRTA).","authors":"Monita Sharma, Erin Huber, Emma Arnesdotter, Holger P Behrsing, Adam Bettmann, David Brandwein, Samuel Constant, Rahul Date, Abhay Deshpande, Eric Fabian, Amit Gupta, Robert Gutierrez, Arno C Gutleb, Marie M Hargrove, Michael Hollings, Victoria Hutter, Annie M Jarabek, Yulia Kaluzhny, Robert Landsiedel, Lawrence Milchak, Robert A Moyer, Jessica R Murray, Kathryn Page, Manish Patel, Stephanie N Pearson, Elijah J Petersen, Emily Reinke, Nuria Roldan, Clive Roper, Jamie B Scaglione, Raja S Settivari, Andreas O Stucki, Sandra Verstraelen, Joanne L Wallace, Shaun McCullough, Amy J Clippinger","doi":"10.1007/s00204-024-03879-z","DOIUrl":null,"url":null,"abstract":"<p><p>Standard information reporting helps to ensure that assay conditions and data are consistently reported and to facilitate inter-laboratory comparisons. Here, we present recommendations on minimum information for reporting on the TEER (trans-epithelial/endothelial electrical resistance) assay (MIRTA). The TEER assay is extensively used to evaluate the health of an epithelial/endothelial cell culture model and as an indicator of the potential toxicity of a test substance. This publication is the result of an international collaboration─called the RespTox (Respiratory Toxicity) Collaborative─through which twelve laboratories shared their protocols for assessing the barrier function of respiratory epithelial cells using the TEER assay following exposure to substances. The protocols from each laboratory were reviewed to identify general steps for performing the TEER assay, interlaboratory differences between steps, the rationale for differences, whether these differences impact results or cross-laboratory comparisons between TEER measurements. While the MIRTA recommendations are focused on respiratory epithelial cell systems, these recommendations can be adapted for other cell systems that form barriers. The use of these recommendations will support data transparency and reproducibility, reduce challenges in data interpretation, enable cross-laboratory comparisons, help assess study quality, and facilitate the incorporation of the TEER assay into national and international testing guidance.</p>","PeriodicalId":8329,"journal":{"name":"Archives of Toxicology","volume":null,"pages":null},"PeriodicalIF":4.8000,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Archives of Toxicology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s00204-024-03879-z","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"TOXICOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Standard information reporting helps to ensure that assay conditions and data are consistently reported and to facilitate inter-laboratory comparisons. Here, we present recommendations on minimum information for reporting on the TEER (trans-epithelial/endothelial electrical resistance) assay (MIRTA). The TEER assay is extensively used to evaluate the health of an epithelial/endothelial cell culture model and as an indicator of the potential toxicity of a test substance. This publication is the result of an international collaboration─called the RespTox (Respiratory Toxicity) Collaborative─through which twelve laboratories shared their protocols for assessing the barrier function of respiratory epithelial cells using the TEER assay following exposure to substances. The protocols from each laboratory were reviewed to identify general steps for performing the TEER assay, interlaboratory differences between steps, the rationale for differences, whether these differences impact results or cross-laboratory comparisons between TEER measurements. While the MIRTA recommendations are focused on respiratory epithelial cell systems, these recommendations can be adapted for other cell systems that form barriers. The use of these recommendations will support data transparency and reproducibility, reduce challenges in data interpretation, enable cross-laboratory comparisons, help assess study quality, and facilitate the incorporation of the TEER assay into national and international testing guidance.
期刊介绍:
Archives of Toxicology provides up-to-date information on the latest advances in toxicology. The journal places particular emphasis on studies relating to defined effects of chemicals and mechanisms of toxicity, including toxic activities at the molecular level, in humans and experimental animals. Coverage includes new insights into analysis and toxicokinetics and into forensic toxicology. Review articles of general interest to toxicologists are an additional important feature of the journal.