Minimum information for reporting on the TEER (trans-epithelial/endothelial electrical resistance) assay (MIRTA).

IF 4.8 2区 医学 Q1 TOXICOLOGY Archives of Toxicology Pub Date : 2024-10-04 DOI:10.1007/s00204-024-03879-z
Monita Sharma, Erin Huber, Emma Arnesdotter, Holger P Behrsing, Adam Bettmann, David Brandwein, Samuel Constant, Rahul Date, Abhay Deshpande, Eric Fabian, Amit Gupta, Robert Gutierrez, Arno C Gutleb, Marie M Hargrove, Michael Hollings, Victoria Hutter, Annie M Jarabek, Yulia Kaluzhny, Robert Landsiedel, Lawrence Milchak, Robert A Moyer, Jessica R Murray, Kathryn Page, Manish Patel, Stephanie N Pearson, Elijah J Petersen, Emily Reinke, Nuria Roldan, Clive Roper, Jamie B Scaglione, Raja S Settivari, Andreas O Stucki, Sandra Verstraelen, Joanne L Wallace, Shaun McCullough, Amy J Clippinger
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Abstract

Standard information reporting helps to ensure that assay conditions and data are consistently reported and to facilitate inter-laboratory comparisons. Here, we present recommendations on minimum information for reporting on the TEER (trans-epithelial/endothelial electrical resistance) assay (MIRTA). The TEER assay is extensively used to evaluate the health of an epithelial/endothelial cell culture model and as an indicator of the potential toxicity of a test substance. This publication is the result of an international collaboration─called the RespTox (Respiratory Toxicity) Collaborative─through which twelve laboratories shared their protocols for assessing the barrier function of respiratory epithelial cells using the TEER assay following exposure to substances. The protocols from each laboratory were reviewed to identify general steps for performing the TEER assay, interlaboratory differences between steps, the rationale for differences, whether these differences impact results or cross-laboratory comparisons between TEER measurements. While the MIRTA recommendations are focused on respiratory epithelial cell systems, these recommendations can be adapted for other cell systems that form barriers. The use of these recommendations will support data transparency and reproducibility, reduce challenges in data interpretation, enable cross-laboratory comparisons, help assess study quality, and facilitate the incorporation of the TEER assay into national and international testing guidance.

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报告 TEER(跨上皮/内皮电阻)测定的最低限度信息(MIRTA)。
标准信息报告有助于确保检测条件和数据报告的一致性,并促进实验室之间的比较。在此,我们就 TEER(跨上皮/内皮电阻)测定(MIRTA)的最低报告信息提出建议。TEER 试验被广泛用于评估上皮/内皮细胞培养模型的健康状况,同时也是检测物质潜在毒性的指标。本出版物是一项国际合作(称为 RespTox(呼吸毒性)合作)的成果。通过这项合作,12 家实验室分享了他们在接触物质后使用 TEER 检测法评估呼吸道上皮细胞屏障功能的方案。对每个实验室的方案都进行了审查,以确定进行 TEER 检测的一般步骤、各步骤之间的实验室间差异、产生差异的理由、这些差异是否会影响结果或 TEER 测量之间的跨实验室比较。虽然 MIRTA 建议的重点是呼吸道上皮细胞系统,但这些建议也可适用于其他形成屏障的细胞系统。使用这些建议将有助于提高数据的透明度和可重复性,减少数据解释方面的挑战,实现跨实验室比较,帮助评估研究质量,并促进将 TEER 分析纳入国家和国际测试指南。
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来源期刊
Archives of Toxicology
Archives of Toxicology 医学-毒理学
CiteScore
11.60
自引率
4.90%
发文量
218
审稿时长
1.5 months
期刊介绍: Archives of Toxicology provides up-to-date information on the latest advances in toxicology. The journal places particular emphasis on studies relating to defined effects of chemicals and mechanisms of toxicity, including toxic activities at the molecular level, in humans and experimental animals. Coverage includes new insights into analysis and toxicokinetics and into forensic toxicology. Review articles of general interest to toxicologists are an additional important feature of the journal.
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