Multiplex molecular assays for the laboratory-based and point-of-care diagnosis of infections caused by seasonal influenza, COVID-19, and RSV.

IF 3.9 3区 医学 Q1 PATHOLOGY Expert Review of Molecular Diagnostics Pub Date : 2024-10-04 DOI:10.1080/14737159.2024.2408745
Alexander Domnich, Elvira Massaro, Giancarlo Icardi, Andrea Orsi
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Abstract

Introduction: SARS-CoV-2, seasonal influenza, and respiratory syncytial virus (RSV) are major causes of acute respiratory infections in all age groups and responsible for an enormous socio-economic burden. The recently coined term 'tripledemic' describes co-circulation of these three viruses, a novel epidemiological paradigm that poses profound public health implications.

Areas covered: Real-time reverse transcription polymerase chain reaction (RT-PCR) is now considered the reference method for the diagnosis of SARS-CoV-2, influenza, and RSV infections. Syndromic-based multiplex RT-PCR panels that simultaneously detect several respiratory viruses have become increasingly common. This review explores available molecular diagnostics (MDx) platforms for the diagnosis of SARS-CoV-2, influenza, and RSV in the same biological sample. Within some limitations of the published validation and diagnostic accuracy studies, both laboratory-based and point-of-care multiplex panels proved highly performant in identifying SARS-CoV-2, influenza A, influenza B, and RSV. Improved operational efficiency and faster turnaround times make these assays potentially cost-effective or even cost-saving.

Expert opinion: The adoption of multiplex MDx assays for the contemporary detection of SARS-CoV-2, influenza, RSV, and other respiratory pathogens will likely increase in the next few years. To maximize the clinical usefulness and cost-effectiveness of these assays, locally issued guidelines and protocols on their implementation should be adopted.

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用于季节性流感、COVID-19 和 RSV 引起的感染的实验室和护理点诊断的多重分子测定。
导言:SARS-CoV-2 、季节性流感和呼吸道合胞病毒 (RSV) 是各年龄段人群急性呼吸道感染的主要病因,造成了巨大的社会经济负担。最近创造的 "三重流行 "一词描述了这三种病毒的共同流行,这是一种新的流行病学模式,对公共卫生产生了深远的影响:实时反转录聚合酶链反应(RT-PCR)目前被认为是诊断 SARS-CoV-2、流感和 RSV 感染的参考方法。同时检测多种呼吸道病毒的基于综合征的多重 RT-PCR 小组越来越常见。本综述探讨了在同一生物样本中诊断 SARS-CoV-2、流感和 RSV 的现有分子诊断(MDx)平台。在已发表的验证和诊断准确性研究的一些限制条件下,基于实验室和护理点的多重检测板在鉴别 SARS-CoV-2、甲型流感、乙型流感和 RSV 方面被证明具有很高的性能。提高操作效率和缩短周转时间使这些检测具有潜在的成本效益,甚至可以节省成本:专家意见:在未来几年中,采用多重 MDx 检测法对 SARS-CoV-2、流感、RSV 和其他呼吸道病原体进行当代检测的情况可能会越来越多。为了最大限度地提高这些检测方法的临床实用性和成本效益,应采用当地发布的实施指南和规程。
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来源期刊
CiteScore
6.60
自引率
0.00%
发文量
71
审稿时长
1 months
期刊介绍: Expert Review of Molecular Diagnostics (ISSN 1473-7159) publishes expert reviews of the latest advancements in the field of molecular diagnostics including the detection and monitoring of the molecular causes of disease that are being translated into groundbreaking diagnostic and prognostic technologies to be used in the clinical diagnostic setting. Each issue of Expert Review of Molecular Diagnostics contains leading reviews on current and emerging topics relating to molecular diagnostics, subject to a rigorous peer review process; editorials discussing contentious issues in the field; diagnostic profiles featuring independent, expert evaluations of diagnostic tests; meeting reports of recent molecular diagnostics conferences and key paper evaluations featuring assessments of significant, recently published articles from specialists in molecular diagnostic therapy. Expert Review of Molecular Diagnostics provides the forum for reporting the critical advances being made in this ever-expanding field, as well as the major challenges ahead in their clinical implementation. The journal delivers this information in concise, at-a-glance article formats: invaluable to a time-constrained community.
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