Essential oils from Cuminum cyminum and Laurus nobilis and their principal constituents: evaluation of antifungal and antimycotoxigenic potential in Aspergillus species.

IF 2.2 4区生物学 Q3 MICROBIOLOGY Fems Microbiology Letters Pub Date : 2024-01-09 DOI:10.1093/femsle/fnae081

Gabriela Aguiar Campolina, Maria das Graças Cardoso, Carolina Salles Freire, Alex Rodrigues Silva Caetano, Anna Beatriz de Souza Campos, Vanuzia Rodrigues Fernandes Ferreira, Eduardo Alves, David Lee Nelson, Luis Roberto Batista

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Abstract

The antifungal and antimycotoxigenic activities of the essential oils (EO) from Cuminum cyminum and Laurus nobilis, and their respective principal compounds, cuminaldehyde and 1,8-cineole, were evaluated against fungi of the genus Aspergillus: A. carbonarius, A. niger, A. ochraceus, and A. westerdijkiae. The antifungal activity was determined by the contact method and the mycelial growth of the fungi was evaluated. Scanning electron microscopic (SEM) images were obtained to suggest modes of action of the compounds analysed. The antimycotoxigenic activity was determined by high-performance liquid chromatograph. Aspergillus carbonarius was completely inhibited by cumin EO (500 µl l-1), by laurel EO and by cuminaldehyde (5000 µl l-1). The cumin EO (500 µl l-1) completely inhibited the growth of A. niger. All the samples inhibited the mycelial growth of A. ochraceus, especially cumin EO and cuminaldehyde (250 µl l-1). Aspergillus westerdijkiae was completely inhibited by cumin EO and cuminaldehyde (1000 µl l-1), by laurel EO and 1,8-cineole (10 000 µl l-1). A decrease in the production of ochratoxin A (OTA) was observed post-treatment, except in A. ochraceus, only inhibited by laurel EO. SEM images showed morphological changes in fungal structures and spore inhibition post-treatment. The results confirmed the antifungal and antimycotoxigenic effect of EO and their principal constituents on fungi evaluated.

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孜然和月桂的精油及其主要成分：对曲霉菌抗真菌和抗霉菌毒素潜力的评估。

研究评估了小茴香和月桂树精油（EO）及其各自的主要化合物（积醛和 1,8-蒎烯）对曲霉属真菌的抗真菌和抗霉菌毒素活性：炭疽曲霉、黑曲霉、赭曲霉和西地那非曲霉。抗真菌活性是通过接触法确定的，并对真菌的菌丝生长情况进行了评估。扫描电子显微镜（SEM）图像显示了所分析化合物的作用模式。高效液相色谱法测定了抗霉菌毒素活性。小茴香环氧乙烷（500 µL L-1）、月桂环氧乙烷和积雪草醛（5000 µL L-1）对 A.carbonarius 具有完全抑制作用。小茴香环氧乙烷（500 µL L-1）完全抑制了黑曲霉的生长。所有样品都能抑制 A.ochraceus 的菌丝生长，尤其是小茴香环氧乙烷和积雪草醛（250 µL L-1）。小茴香环氧乙烷和积雪草醛（1000 µL L-1）、月桂环氧乙烷和 1,8- 丁烯醚（10000 µL L-1）可完全抑制赭曲霉。除赭曲霉只受月桂环氧乙烷抑制外，其他真菌在处理后都减少了赭曲霉毒素 A（OTA）的产生。扫描电子显微镜图像显示了处理后真菌结构的形态变化和孢子抑制作用。结果证实了环氧乙烷及其主要成分对真菌的抗真菌和抗霉菌毒素作用。

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来源期刊

Fems Microbiology Letters 生物-微生物学

CiteScore

4.30

自引率

0.00%

发文量

112

审稿时长

1.9 months

期刊介绍： FEMS Microbiology Letters gives priority to concise papers that merit rapid publication by virtue of their originality, general interest and contribution to new developments in microbiology. All aspects of microbiology, including virology, are covered. 2019 Impact Factor: 1.987, Journal Citation Reports (Source Clarivate, 2020) Ranking: 98/135 (Microbiology) The journal is divided into eight Sections: Physiology and Biochemistry (including genetics, molecular biology and ‘omic’ studies) Food Microbiology (from food production and biotechnology to spoilage and food borne pathogens) Biotechnology and Synthetic Biology Pathogens and Pathogenicity (including medical, veterinary, plant and insect pathogens – particularly those relating to food security – with the exception of viruses) Environmental Microbiology (including ecophysiology, ecogenomics and meta-omic studies) Virology (viruses infecting any organism, including Bacteria and Archaea) Taxonomy and Systematics (for publication of novel taxa, taxonomic reclassifications and reviews of a taxonomic nature) Professional Development (including education, training, CPD, research assessment frameworks, research and publication metrics, best-practice, careers and history of microbiology) If you are unsure which Section is most appropriate for your manuscript, for example in the case of transdisciplinary studies, we recommend that you contact the Editor-In-Chief by email prior to submission. Our scope includes any type of microorganism - all members of the Bacteria and the Archaea and microbial members of the Eukarya (yeasts, filamentous fungi, microbial algae, protozoa, oomycetes, myxomycetes, etc.) as well as all viruses.