Benfotiamine Supplementation Increases Thiamine in Muscle of Endurance-Trained Mice and Affects the Energy Metabolism.

IF 2.3 Q3 NUTRITION & DIETETICS Journal of Nutrition and Metabolism Pub Date : 2024-09-26 eCollection Date: 2024-01-01 DOI:10.1155/2024/6102611
Álisson C Gonçalves, Jéssica F Vieira, Ana Carolina N Rodrigues, Eddie F C Murta, Júlio S Marchini, Márcia A Michelin, Guilherme V Portari
{"title":"Benfotiamine Supplementation Increases Thiamine in Muscle of Endurance-Trained Mice and Affects the Energy Metabolism.","authors":"Álisson C Gonçalves, Jéssica F Vieira, Ana Carolina N Rodrigues, Eddie F C Murta, Júlio S Marchini, Márcia A Michelin, Guilherme V Portari","doi":"10.1155/2024/6102611","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Benfotiamine, a synthetic analog of thiamine, offers greater bioavailability compared to other thiamine salts and increases thiamine stores upon oral intake. Thiamine is essential for energy metabolism. This study aimed to evaluate the effects of oral benfotiamine supplementation on energy metabolism, particularly the Krebs cycle function, in the muscle of endurance-trained mice, and to assess its impact on endurance performance.</p><p><strong>Methods: </strong>Twenty-five mice were randomly assigned to four groups: a standard diet with sedentary behavior (Sta-Sed), a benfotiamine-supplemented diet with sedentary behavior (Ben-Sed), a standard diet with swimming training (Sta-Tr), and a benfotiamine-supplemented diet with swimming training (Ben-Tr). The trained groups underwent five weekly swimming sessions for six weeks, followed by an exhaustive test. Thiamine and its esters were measured in erythrocytes and gastrocnemius muscle. Gene expression of pyruvate dehydrogenase (PDHa) and alpha-ketoglutarate dehydrogenase (OGDH), along with levels of pyruvic, lactic, and hydroxybutyric acids in muscle, was analyzed.</p><p><strong>Results: </strong>The benfotiamine-supplemented groups had higher thiamine levels in erythrocytes and muscles compared to the standard-diet groups. No differences were observed in PDHa and OGDH gene expression. The Ben-Tr group exhibited increased muscle lactic acid levels and a higher lactic acid to pyruvic acid ratio compared to the sedentary groups. Hydroxybutyric acid levels were also elevated in the Ben-Tr group. No significant differences in exhaustive test duration were found between the groups.</p><p><strong>Conclusion: </strong>Benfotiamine supplementation increases thiamine levels in erythrocytes and muscle but does not affect the gene expression of thiamine-dependent enzymes. Although it alters energy metabolism in trained muscle, it does not enhance endurance performance in mice.</p>","PeriodicalId":16587,"journal":{"name":"Journal of Nutrition and Metabolism","volume":null,"pages":null},"PeriodicalIF":2.3000,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11449553/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Nutrition and Metabolism","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1155/2024/6102611","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"NUTRITION & DIETETICS","Score":null,"Total":0}
引用次数: 0

Abstract

Background: Benfotiamine, a synthetic analog of thiamine, offers greater bioavailability compared to other thiamine salts and increases thiamine stores upon oral intake. Thiamine is essential for energy metabolism. This study aimed to evaluate the effects of oral benfotiamine supplementation on energy metabolism, particularly the Krebs cycle function, in the muscle of endurance-trained mice, and to assess its impact on endurance performance.

Methods: Twenty-five mice were randomly assigned to four groups: a standard diet with sedentary behavior (Sta-Sed), a benfotiamine-supplemented diet with sedentary behavior (Ben-Sed), a standard diet with swimming training (Sta-Tr), and a benfotiamine-supplemented diet with swimming training (Ben-Tr). The trained groups underwent five weekly swimming sessions for six weeks, followed by an exhaustive test. Thiamine and its esters were measured in erythrocytes and gastrocnemius muscle. Gene expression of pyruvate dehydrogenase (PDHa) and alpha-ketoglutarate dehydrogenase (OGDH), along with levels of pyruvic, lactic, and hydroxybutyric acids in muscle, was analyzed.

Results: The benfotiamine-supplemented groups had higher thiamine levels in erythrocytes and muscles compared to the standard-diet groups. No differences were observed in PDHa and OGDH gene expression. The Ben-Tr group exhibited increased muscle lactic acid levels and a higher lactic acid to pyruvic acid ratio compared to the sedentary groups. Hydroxybutyric acid levels were also elevated in the Ben-Tr group. No significant differences in exhaustive test duration were found between the groups.

Conclusion: Benfotiamine supplementation increases thiamine levels in erythrocytes and muscle but does not affect the gene expression of thiamine-dependent enzymes. Although it alters energy metabolism in trained muscle, it does not enhance endurance performance in mice.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
补充苯磷硫胺可增加耐力训练小鼠肌肉中的硫胺素含量并影响能量代谢
背景:苯磷硫胺是硫胺素的合成类似物,与其他硫胺素盐相比,生物利用度更高,口服后可增加硫胺素的储存量。硫胺素对能量代谢至关重要。本研究旨在评估口服苯磷硫胺对耐力训练小鼠肌肉中能量代谢,尤其是克雷布斯循环功能的影响,并评估其对耐力表现的影响:25只小鼠被随机分配到四组:标准饮食与久坐不动组(Sta-Sed)、补充苯磷硫胺饮食与久坐不动组(Ben-Sed)、标准饮食与游泳训练组(Sta-Tr)和补充苯磷硫胺饮食与游泳训练组(Ben-Tr)。训练组每周进行五次游泳训练,为期六周,然后进行一次力竭测试。测量了红细胞和腓肠肌中的硫胺素及其酯类。分析了丙酮酸脱氢酶(PDHa)和α-酮戊二酸脱氢酶(OGDH)的基因表达以及肌肉中丙酮酸、乳酸和羟丁酸的水平:结果:与标准饮食组相比,补充苯磷硫胺素组红细胞和肌肉中的硫胺素水平更高。在 PDHa 和 OGDH 基因表达方面未观察到差异。与久坐组相比,Ben-Tr 组的肌肉乳酸水平升高,乳酸与丙酮酸的比率升高。Ben-Tr 组的羟丁酸水平也有所升高。结论:补充苯磷硫胺可增加硫酸锌的摄入量:结论:补充苯磷硫胺可提高红细胞和肌肉中的硫胺水平,但不会影响硫胺依赖酶的基因表达。虽然它能改变训练肌肉的能量代谢,但并不能提高小鼠的耐力表现。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Journal of Nutrition and Metabolism
Journal of Nutrition and Metabolism NUTRITION & DIETETICS-
CiteScore
5.40
自引率
0.00%
发文量
49
审稿时长
17 weeks
期刊介绍: Journal of Nutrition and Metabolism is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies covering the broad and multidisciplinary field of human nutrition and metabolism. The journal welcomes submissions on studies related to obesity, diabetes, metabolic syndrome, molecular and cellular biology of nutrients, foods and dietary supplements, as well as macro- and micronutrients including vitamins and minerals.
期刊最新文献
Exploring the Role of Licorice and Its Derivatives in Cell Signaling Pathway NF-κB and MAPK. Ketogenic Diet: A Review of Composition Diversity, Mechanism of Action and Clinical Application. The Impact of Short-Term Supplementation With Guanidinoacetic Acid and Creatine Versus Creatine Alone on Body Composition Indices in Healthy Men and Women: Creatine-Guanidinoacetic Acid Affects Body Composition. Vitamin D Status and Physical Functioning in Nursing Home Residents after Improved Adherence to the Vitamin D and Calcium Recommendation-A Quasiexperimental Study. High Incidence of Refeeding Syndrome during the Transition from F75 to Ready-to-Use Therapeutic Feeds among Children 6 to 59 Months with Severe Acute Malnutrition at the Pediatric Nutritional Unit of Mulago Hospital.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1