Lectin histochemistry in the small intestines of piglets naturally infected with porcine epidemic diarrhea virus.

IF 1.5 3区 农林科学 Q2 VETERINARY SCIENCES Journal of Veterinary Science Pub Date : 2024-09-01 DOI:10.4142/jvs.24179
Bohye Kim, Sungwoong Jang, Hyewon Jang, Joong-Sun Kim, Tae-Il Jeon, Jun-Gyu Park, In-Sik Shin, Kyoung-Oh Cho, Changjong Moon
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Abstract

Importance: Porcine epidemic diarrhea virus (PEDV) binds to particular cell surface receptors to penetrate cells. The virus specifically identifies certain carbohydrate structures present on the surface of the cell to facilitate the binding process. Nevertheless, the influence of viral infections on specific alterations of glycoconjugates in the small intestines remains unexplored.

Objective: This work aimed to examine the alterations in glycoconjugates in the small intestines of piglets naturally infected with PEDV using lectin histochemistry.

Methods: Six piglets including three PEDV-infected and three non-infected piglets were evaluated. Small intestinal samples were histopathologically examined, and lectin histochemistry was performed.

Results: Piglets infected with PEDV had significant histological abnormalities in their small intestines, such as pronounced villous atrophy, varying degrees of villous fusion, and diverse mucosal alterations. Specific regions of the duodenum, jejunum, and ileum showed discernible variations in glycoconjugate distribution, as determined by lectin histochemistry. Compared with the controls, the PEDV-infected piglets showed significant changes in N-acetylglucosamine- and galactose-binding lectins (particularly wheat germ agglutinin and Arachis hypogaea (peanut) agglutinin) in multiple intestinal regions.

Conclusions and relevance: These findings can enhance understanding of how viruses such as PEDV impact the glycoconjugate composition of the small intestines and emphasize the potential connection between the pathogenesis of PEDV and glycoconjugate.

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猪流行性腹泻病毒自然感染仔猪小肠中的凝集素组织化学。
重要性:猪流行性腹泻病毒(PEDV)会与特定的细胞表面受体结合,从而穿透细胞。病毒会专门识别细胞表面的某些碳水化合物结构,以促进结合过程。然而,病毒感染对小肠中糖结合物的特定改变的影响仍未得到研究:本研究旨在利用凝集素组织化学法研究自然感染 PEDV 的仔猪小肠中糖结合物的变化:方法:对六头仔猪进行了评估,其中包括三头感染 PEDV 的仔猪和三头未感染 PEDV 的仔猪。对小肠样本进行组织病理学检查,并进行凝集素组织化学分析:结果:感染了 PEDV 的仔猪的小肠组织出现了明显的异常,如绒毛明显萎缩、不同程度的绒毛融合以及多种粘膜改变。根据凝集素组织化学测定,十二指肠、空肠和回肠的特定区域显示出明显的糖原分布变化。与对照组相比,PEDV 感染仔猪的多个肠道区域的 N-乙酰葡糖胺和半乳糖结合凝集素(尤其是小麦胚芽凝集素和花生凝集素)发生了显著变化:这些发现可加深人们对 PEDV 等病毒如何影响小肠糖凝集素组成的理解,并强调 PEDV 的致病机理与糖凝集素之间的潜在联系。
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来源期刊
Journal of Veterinary Science
Journal of Veterinary Science 农林科学-兽医学
CiteScore
3.10
自引率
5.60%
发文量
86
审稿时长
1.3 months
期刊介绍: The Journal of Veterinary Science (J Vet Sci) is devoted to the advancement and dissemination of scientific knowledge concerning veterinary sciences and related academic disciplines. It is an international journal indexed in the Thomson Scientific Web of Science, SCI-EXPANDED, Sci Search, BIOSIS Previews, Biological Abstracts, Focus on: Veterinary Science & Medicine, Zoological Record, PubMed /MEDLINE, Index Medicus, Pubmed Central, CAB Abstracts / Index Veterinarius, EBSCO, AGRIS and AGRICOLA. This journal published in English by the Korean Society of Veterinary Science (KSVS) being distributed worldwide.
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