Molecular characteristics and antimicrobial susceptibility profiles of bovine mastitis agents in western Türkiye.

IF 1.5 3区 农林科学 Q2 VETERINARY SCIENCES Journal of Veterinary Science Pub Date : 2024-09-01 DOI:10.4142/jvs.24032
Semiha Yalcin, Arzu Ozgen, Metehan Simsir
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Abstract

Importance: Identifying bovine mastitis agents using molecular methods to reveal their phylogenetic relationships and antimicrobial resistance profiles is essential for developing up-to-date databases in mastitis cases that cause severe economic losses.

Objective: This study examined bacterial mastitis agents in cows with clinical and subclinical mastitis observed in various dairy cattle farms to reveal their phylogenetic relationships and antibiotic resistance properties.

Methods: Sixty-two clinical and subclinical bovine mastitis milk samples were collected from 15 dairy farms. The polymerase chain reaction (PCR) was used to amplify the 16S rRNA gene regions of the bacteria. The 16S rRNA gene sequences obtained from sequencing include the V4-V6 regions. The strains were compared using a similarity analysis method that produced phylogenetic trees using the Molecular Evolutionary Genetics Analysis 11 program. Antibiotic susceptibilities were determined using the Kirby-Bauer disk diffusion method.

Results: Sixty-three bacteria were isolated and identified in this study. The most isolated bacteria from all mastitis cases were Staphylococcus spp. (30.2%), Escherichia coli (25.4%), Streptococcus spp. (14.3%), and Aerococcus spp. (7.9%), respectively. The phylogenetic trees were drawn from the 16S rRNA sequences. Some of these bacteria showed resistance to different types of antibiotics at varying rates.

Conclusions and relevance: The bacteria isolated in this study originated from environmental sources. Regular cleaning of barns and proper hygiene practices are essential. Regular screenings for mastitis should be conducted in herds instead of the random or empirical use of antibiotics.

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图尔基耶西部牛乳腺炎病原体的分子特征和抗菌药敏感性概况。
重要性:使用分子方法鉴定牛乳腺炎病原体,以揭示其系统发育关系和抗菌药耐药性特征,对于建立造成严重经济损失的乳腺炎病例的最新数据库至关重要:本研究对在不同奶牛场观察到的患有临床和亚临床乳腺炎的奶牛中的细菌性乳腺炎病原体进行了研究,以揭示其系统发育关系和抗生素耐药性特性:从 15 个奶牛场收集了 62 份临床和亚临床牛乳腺炎牛奶样本。采用聚合酶链反应(PCR)扩增细菌的 16S rRNA 基因区。测序获得的 16S rRNA 基因序列包括 V4-V6 区域。采用相似性分析方法对菌株进行比较,该方法利用分子进化遗传学分析 11 程序生成系统发生树。采用柯比鲍尔盘扩散法测定抗生素敏感性:本研究分离并鉴定了 63 种细菌。从所有乳腺炎病例中分离出最多的细菌分别是葡萄球菌属(30.2%)、大肠杆菌属(25.4%)、链球菌属(14.3%)和气球菌属(7.9%)。系统发生树是根据 16S rRNA 序列绘制的。其中一些细菌对不同类型的抗生素表现出不同程度的耐药性:本研究中分离出的细菌来源于环境。定期清洁牛舍和正确的卫生习惯至关重要。应定期对牛群进行乳腺炎筛查,而不是随意或凭经验使用抗生素。
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来源期刊
Journal of Veterinary Science
Journal of Veterinary Science 农林科学-兽医学
CiteScore
3.10
自引率
5.60%
发文量
86
审稿时长
1.3 months
期刊介绍: The Journal of Veterinary Science (J Vet Sci) is devoted to the advancement and dissemination of scientific knowledge concerning veterinary sciences and related academic disciplines. It is an international journal indexed in the Thomson Scientific Web of Science, SCI-EXPANDED, Sci Search, BIOSIS Previews, Biological Abstracts, Focus on: Veterinary Science & Medicine, Zoological Record, PubMed /MEDLINE, Index Medicus, Pubmed Central, CAB Abstracts / Index Veterinarius, EBSCO, AGRIS and AGRICOLA. This journal published in English by the Korean Society of Veterinary Science (KSVS) being distributed worldwide.
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