Molecular detection of high-risk papillomaviruses and vaccination status in normal cytology in Congo.

Q3 Medicine Voprosy virusologii Pub Date : 2024-09-26 DOI:10.36233/0507-4088-239

D F Ngombe Mouabata, A L Boumba, P J Iloukou Mayakia, N R Massengo, R P Takale, D Moukassa, M M Ennaji

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Abstract

Objective: The aim of this study was to identify the molecular prevalence of high-risk HPV infection and the distribution of genotypes present in normal cytology, as well as to determine the vaccination status of our study population.

Methods: 110 cervical samples were taken from individuals, and 1 ml of each sample was added to the Xpert HPV cartridge in the sample compartment before it was placed in the Cepheid GeneXpert system. Detection was performed simultaneously via amplification of the E6 and E7 genes in five fluorescent channels (HPV16, HPV18/45, HPV31/33/35/52/58, HPV51/59, and HPV39/56/66/68a).

Results: 36/110 (33%) of all samples tested were positive for HPV DNA. The predominant genotypes were HPV16 (12.7%) and other pooled HR-HPV types (8.2%). All women who received the Gardasil-9 vaccine (3.6%) had HPV, and infection was associated with travel outside Africa. 96.4% of the screened individuals had not received any HPV vaccine.

Conclusion: Our research confirms a widespread HR-HPV infection in our population and extends the importance of studies on the molecular prevalence of HPV, particularly in women with normal cytology and apparent good health, in view of the cruel lack of public awareness of HPV infections.

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刚果正常细胞学中高危乳头瘤病毒的分子检测和疫苗接种情况。

研究目的本研究旨在确定高危 HPV 感染的分子流行率和正常细胞学检查中出现的基因型的分布情况，并确定研究人群的疫苗接种情况。方法：从个人身上采集 110 份宫颈样本，每份样本在放入 Cepheid GeneXpert 系统之前，先将 1 毫升样本加入样本仓中的 Xpert HPV 盒。检测是通过扩增五个荧光通道（HPV16、HPV18/45、HPV31/33/35/52/58、HPV51/59 和 HPV39/56/66/68a）中的 E6 和 E7 基因同时进行的：在所有检测样本中，36/110（33%）个样本的 HPV DNA 呈阳性。主要基因型为 HPV16（12.7%）和其他集中的 HR-HPV 类型（8.2%）。所有接种加德西-9 疫苗的女性（3.6%）都感染了 HPV，感染与非洲以外的旅行有关。96.4%的受检者未接种过任何HPV疫苗：我们的研究证实，在我们的人群中，HR-HPV 感染非常普遍，鉴于公众对 HPV 感染的认识严重不足，对 HPV 分子流行率进行研究，尤其是对细胞学检查正常且健康状况明显良好的妇女进行研究，显得尤为重要。

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来源期刊

Voprosy virusologii Medicine-Infectious Diseases

CiteScore

2.00

自引率

0.00%

发文量

期刊介绍： The journal deals with advances in virology in Russia and abroad. It publishes papers dealing with investigations of viral diseases of man, animals and plants, the results of experimental research on different problems of general and special virology. The journal publishes materials are which promote introduction into practice of the achievements of the virological science in the eradication and incidence reduction of infectious diseases, as well as their diagnosis, treatment and prevention. The reader will find a description of new methods of investigation, new apparatus and devices.