Special AT-rich sequence-binding protein 2 immunohistochemistry in the diagnosis of osteosarcoma in dogs

IF 0.8 4区 农林科学 Q4 PATHOLOGY Journal of Comparative Pathology Pub Date : 2024-10-04 DOI:10.1016/j.jcpa.2024.09.001
{"title":"Special AT-rich sequence-binding protein 2 immunohistochemistry in the diagnosis of osteosarcoma in dogs","authors":"","doi":"10.1016/j.jcpa.2024.09.001","DOIUrl":null,"url":null,"abstract":"<div><div>Malignant osteoblasts can have markedly pleomorphic phenotypes and variable amounts of tumour-associated matrix, complicating the ability of pathologists to accurately differentiate osteosarcoma (OSA) from other types of neoplasms using only histopathology. Current immunohistochemical markers for animals have limited sensitivity and specificity in identifying OSA or produce inconsistent results. Immunohistochemistry (IHC) for special AT-rich sequence-binding protein 2 (SATB2) has been used in human medicine to aid in identification of normal and neoplastic osteoblasts, and the objective of this study was to determine whether this marker could also be useful for the diagnosis of canine OSA. Initially, SATB2 IHC was performed on eight samples from cases of well-differentiated canine OSA and on other tumour types for which OSA is a differential diagnosis, as well as on normal tissues, to assess sensitivity and cross-reactivity. Following confirmation that SATB2 is immunoreactive for normal and neoplastic canine osteoblasts and negative in other non-osseous mesenchymal cell types and organs, SATB2 IHC was tested on 123 cases of poorly differentiated malignant neoplasms as part of a panel with other immunohistochemical markers, as appropriate, based on histomorphology and differential diagnoses. The conclusion is that SATB2 IHC is a sensitive and specific marker for identifying canine OSA when used in a panel with other immunohistochemical markers and in conjunction with supportive clinical history.</div></div>","PeriodicalId":15520,"journal":{"name":"Journal of Comparative Pathology","volume":null,"pages":null},"PeriodicalIF":0.8000,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Comparative Pathology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0021997524003049","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PATHOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Malignant osteoblasts can have markedly pleomorphic phenotypes and variable amounts of tumour-associated matrix, complicating the ability of pathologists to accurately differentiate osteosarcoma (OSA) from other types of neoplasms using only histopathology. Current immunohistochemical markers for animals have limited sensitivity and specificity in identifying OSA or produce inconsistent results. Immunohistochemistry (IHC) for special AT-rich sequence-binding protein 2 (SATB2) has been used in human medicine to aid in identification of normal and neoplastic osteoblasts, and the objective of this study was to determine whether this marker could also be useful for the diagnosis of canine OSA. Initially, SATB2 IHC was performed on eight samples from cases of well-differentiated canine OSA and on other tumour types for which OSA is a differential diagnosis, as well as on normal tissues, to assess sensitivity and cross-reactivity. Following confirmation that SATB2 is immunoreactive for normal and neoplastic canine osteoblasts and negative in other non-osseous mesenchymal cell types and organs, SATB2 IHC was tested on 123 cases of poorly differentiated malignant neoplasms as part of a panel with other immunohistochemical markers, as appropriate, based on histomorphology and differential diagnoses. The conclusion is that SATB2 IHC is a sensitive and specific marker for identifying canine OSA when used in a panel with other immunohistochemical markers and in conjunction with supportive clinical history.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
特殊富AT序列结合蛋白2免疫组化在犬骨肉瘤诊断中的应用。
恶性成骨细胞可能具有明显的多形性表型和不同数量的肿瘤相关基质,这使得病理学家仅通过组织病理学准确区分骨肉瘤(OSA)和其他类型肿瘤的能力变得更加复杂。目前用于动物的免疫组化标记物在鉴别 OSA 方面的灵敏度和特异性有限,或者产生的结果不一致。特殊富AT序列结合蛋白2(SATB2)的免疫组化(IHC)已在人类医学中用于帮助鉴别正常和肿瘤性成骨细胞,本研究的目的是确定该标记物是否也可用于犬OSA的诊断。首先,对来自分化良好的犬 OSA 病例、OSA 作为鉴别诊断的其他肿瘤类型以及正常组织的八个样本进行了 SATB2 IHC 检测,以评估敏感性和交叉反应性。在确认 SATB2 对正常和肿瘤性犬成骨细胞有免疫反应,而对其他非骨质间充质细胞类型和器官呈阴性反应后,对 123 例分化不良的恶性肿瘤进行了 SATB2 IHC 检测,并根据组织形态学和鉴别诊断结果,酌情将其与其他免疫组化标记物一起作为检测组的一部分。结论是,SATB2 IHC 与其他免疫组化标记物一起使用,并结合辅助性临床病史,是鉴别犬 OSA 的敏感而特异的标记物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
CiteScore
1.60
自引率
0.00%
发文量
208
审稿时长
50 days
期刊介绍: The Journal of Comparative Pathology is an International, English language, peer-reviewed journal which publishes full length articles, short papers and review articles of high scientific quality on all aspects of the pathology of the diseases of domesticated and other vertebrate animals. Articles on human diseases are also included if they present features of special interest when viewed against the general background of vertebrate pathology.
期刊最新文献
A case of epithelioid glioblastoma with lung metastases in a young Cane Corso dog Intra-abdominal heterotopic mesenteric ossification associated with intestinal volvulus in a sow (Sus scrofa domesticus) Intravascular central nervous system T-cell lymphoma in a common dolphin (Delphinus delphis) with cerebral infarction Annual congress of the European Society of Veterinary Pathology and the European College of Veterinary Pathologists Special AT-rich sequence-binding protein 2 immunohistochemistry in the diagnosis of osteosarcoma in dogs
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1