Electrospun "Hard-Soft" Interpenetrating Nanofibrous Tissue Scaffolds Facilitating Enhanced Mechanical Strength and Cell Proliferation.

IF 5.4 2区 医学 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Biomaterials Science & Engineering Pub Date : 2024-11-11 Epub Date: 2024-10-05 DOI:10.1021/acsbiomaterials.4c00650
Samaneh Toufanian, Mya Sharma, Fei Xu, Seyed Saeid Tayebi, Christina McCabe, Elaina Piliouras, Todd Hoare
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Abstract

"Soft" hydrogel-based macroporous scaffolds have been widely used in tissue engineering and drug delivery applications due to their hydrated interfaces and macroporous structures, but have drawbacks related to their weak mechanics and often weak adhesion to cells. In contrast, "hard" poly(caprolactone) (PCL) electrospun fibrous networks have desirable mechanical strength and ductility but offer minimal interfacial hydration and thus limited capacity for cell proliferation. Herein, we demonstrate the fabrication of interpenetrating nanofibrous networks based on coelectrospun PCL and poly(oligoethylene glycol methacrylate) (POEGMA) nanofibers that exhibit the mechanical benefits of PCL but the interfacial hydration benefits of hydrogels. The electrospinning process results in partially aligned but interpenetrating fiber network with minimal internal phase separation, leading to anisotropic but strong mechanical properties even in the hydrated state; apparent ultimate tensile strengths of the swollen scaffolds ranged from 429 ± 39 kPa in the direction of fiber alignment (longitudinal) to 86 ± 25 kPa perpendicular to fiber alignment (cross-longitudinal), typical of PCL-based scaffolds and enabling efficient suture retention in different directions. However, contact angle measurements indicate hydrogel-like interfacial properties due to the presence of the interpenetrating POEGMA network. C2C12 myoblast proliferation in the PCL-POEGMA scaffolds was 50% higher than that observed on PCL-only scaffolds, a result attributed to the presence of the more hydrophilic POEGMA interpenetrating nanofiber network. Overall, this method is demonstrated to represent a facile single-step strategy to fabricate strong macroporous but still interfacially hydrophilic scaffolds for tissue engineering applications.

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电纺 "硬-软 "互穿纳米纤维组织支架有助于增强机械强度和细胞增殖。
基于水凝胶的 "软 "大孔支架因其水合界面和大孔结构而被广泛应用于组织工程和药物输送领域,但其缺点是力学性能较弱,对细胞的粘附力也较弱。相比之下,"硬 "聚(己内酯)(PCL)电纺纤维网络具有理想的机械强度和延展性,但其界面水合作用极小,因此细胞增殖能力有限。在此,我们展示了基于 PCL 和聚(低聚乙二醇甲基丙烯酸酯)(POEGMA)共电纺纳米纤维的互穿纳米纤维网络的制造工艺,该工艺既具有 PCL 的机械性能,又具有水凝胶的界面水合性能。电纺丝过程产生了部分排列但相互渗透的纤维网,内部相分离极少,因此即使在水合状态下也具有各向异性但很强的机械性能;膨胀支架的表观极限拉伸强度从纤维排列方向(纵向)的 429 ± 39 kPa 到垂直于纤维排列方向(横纵向)的 86 ± 25 kPa 不等,是典型的 PCL 基支架,可实现不同方向的高效缝合固定。然而,接触角测量结果表明,由于存在相互渗透的 POEGMA 网络,该材料具有类似水凝胶的界面特性。C2C12成肌细胞在 PCL-POEGMA 支架中的增殖率比在纯 PCL 支架上观察到的增殖率高 50%,这一结果归因于存在亲水性更强的 POEGMA 互穿纳米纤维网络。总之,这种方法代表了一种简便的单步策略,可为组织工程应用制造强力大孔但仍具有界面亲水性的支架。
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来源期刊
ACS Biomaterials Science & Engineering
ACS Biomaterials Science & Engineering Materials Science-Biomaterials
CiteScore
10.30
自引率
3.40%
发文量
413
期刊介绍: ACS Biomaterials Science & Engineering is the leading journal in the field of biomaterials, serving as an international forum for publishing cutting-edge research and innovative ideas on a broad range of topics: Applications and Health – implantable tissues and devices, prosthesis, health risks, toxicology Bio-interactions and Bio-compatibility – material-biology interactions, chemical/morphological/structural communication, mechanobiology, signaling and biological responses, immuno-engineering, calcification, coatings, corrosion and degradation of biomaterials and devices, biophysical regulation of cell functions Characterization, Synthesis, and Modification – new biomaterials, bioinspired and biomimetic approaches to biomaterials, exploiting structural hierarchy and architectural control, combinatorial strategies for biomaterials discovery, genetic biomaterials design, synthetic biology, new composite systems, bionics, polymer synthesis Controlled Release and Delivery Systems – biomaterial-based drug and gene delivery, bio-responsive delivery of regulatory molecules, pharmaceutical engineering Healthcare Advances – clinical translation, regulatory issues, patient safety, emerging trends Imaging and Diagnostics – imaging agents and probes, theranostics, biosensors, monitoring Manufacturing and Technology – 3D printing, inks, organ-on-a-chip, bioreactor/perfusion systems, microdevices, BioMEMS, optics and electronics interfaces with biomaterials, systems integration Modeling and Informatics Tools – scaling methods to guide biomaterial design, predictive algorithms for structure-function, biomechanics, integrating bioinformatics with biomaterials discovery, metabolomics in the context of biomaterials Tissue Engineering and Regenerative Medicine – basic and applied studies, cell therapies, scaffolds, vascularization, bioartificial organs, transplantation and functionality, cellular agriculture
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