Exploring Anticancer Potential of Lactobacillus Strains: Insights into Cytotoxicity and Apoptotic Mechanisms on HCT 115 Cancer Cells.

IF 5.3 Q1 MEDICINE, RESEARCH & EXPERIMENTAL Biologics : Targets & Therapy Pub Date : 2024-10-01 eCollection Date: 2024-01-01 DOI:10.2147/BTT.S477602
Luolin Wang, Zhenglei Xu, Aarti Bains, Nemat Ali, Zifang Shang, Abhinandan Patil, Sandip Patil
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Abstract

Introduction: This study aims to systematically assess the anticancer potential of distinct Lactobacillus strains on Human Colorectal Tumor (HCT) 115 cancer cells, with a primary focus on the apoptotic mechanisms involved. Lactobacillus strains were isolated from sheep milk and underwent a meticulous microbial isolation process. Previous research indicates that certain probiotic bacteria, including Lactobacillus species, may exhibit anticancer properties through mechanisms such as apoptosis induction. However, there is limited understanding of how different Lactobacillus strains exert these effects on cancer cells and the underlying molecular pathways involved.

Methods: Cytotoxicity was evaluated through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays and exposure durations of Lactobacillus cell-free lyophilized filtrates. Additional apoptotic features were characterized using 4.6-diamidino-2-phenylindole (DAPI) analysis for nuclear fragmentation and Annexin V/PI analysis for apoptosis quantification. Genetic analysis explored the modulation of apoptotic proteins (Bax and Bcl2) in response to Lactobacillus treatment. Whole-genome sequencing (WGS) was performed to understand the genetic makeup of the Lactobacillus strains used in the study.

Results: The study demonstrated a significant reduction in HCT 115 cell viability, particularly with L. plantarum, as evidenced by Sulforhodamine B (SRB) and MTT assays. DAPI analysis revealed nuclear fragmentation, emphasizing an apoptotic cell death mechanism. Annexin V/PI analysis supported this, showing a higher percentage of early and late apoptosis in L. plantarum-treated cells. Genetic analysis uncovered up-regulation of pro-apoptotic protein Bax and down-regulation of anti-apoptotic protein Bcl2 in response to Lactobacillus treatment. WGS study revealed a strain reported to NCBI PRJNA439183.

Discussion: L. plantarum emerged as a potent antiproliferative agent against HCT 115 cancer cells, inducing apoptosis through intricate molecular mechanisms. This study underscores the scientific basis for L. plantarum's potential role in cancer therapeutics, highlighting its impact on antiproliferation, adhesion, and gene-protein regulation. Further research is warranted to elucidate the specific molecular pathways involved and to evaluate the therapeutic potential of L. plantarum in preclinical and clinical settings.

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探索乳酸杆菌菌株的抗癌潜力:洞察 HCT 115 癌细胞的细胞毒性和凋亡机制
简介:本研究旨在系统评估不同乳酸杆菌菌株对人类结直肠肿瘤(HCT)115癌细胞的抗癌潜力,主要关注其中的凋亡机制。乳酸杆菌菌株是从羊奶中分离出来的,并经过了严格的微生物分离过程。以往的研究表明,包括乳酸杆菌在内的某些益生菌可能通过诱导细胞凋亡等机制表现出抗癌特性。然而,人们对不同乳酸杆菌菌株如何对癌细胞产生这些影响以及所涉及的潜在分子途径的了解还很有限:方法:通过 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四氮唑(MTT)测定法和乳酸菌无细胞冻干滤液的暴露时间来评估细胞毒性。此外,还使用 4.6-二脒基-2-苯基吲哚(DAPI)分析法对细胞核碎片进行分析,并使用 Annexin V/PI 分析法对细胞凋亡进行定量。遗传分析探讨了凋亡蛋白(Bax 和 Bcl2)在乳酸菌处理中的变化。进行了全基因组测序(WGS),以了解研究中使用的乳酸杆菌菌株的基因构成:研究表明,植物乳杆菌能显著降低 HCT 115 细胞的存活率,这一点在磺基罗丹明 B(SRB)和 MTT 检测中得到了证明。DAPI 分析显示细胞核破碎,强调了细胞凋亡机制。Annexin V/PI 分析证实了这一点,显示植物乳杆菌处理过的细胞早期和晚期凋亡的比例更高。基因分析发现,乳酸杆菌处理后,促凋亡蛋白 Bax 上调,而抗凋亡蛋白 Bcl2 下调。WGS研究显示,NCBI PRJNA439183报告了一株菌株:讨论:植物乳杆菌通过复杂的分子机制诱导癌细胞凋亡,成为一种有效的抗 HCT 115 癌细胞增殖剂。这项研究强调了植物乳杆菌在癌症治疗中潜在作用的科学依据,突出了它对抗增殖、粘附和基因蛋白调控的影响。我们有必要开展进一步的研究,以阐明其中涉及的具体分子途径,并评估植物乳杆菌在临床前和临床环境中的治疗潜力。
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来源期刊
Biologics : Targets & Therapy
Biologics : Targets & Therapy MEDICINE, RESEARCH & EXPERIMENTAL-
CiteScore
8.30
自引率
0.00%
发文量
22
审稿时长
16 weeks
期刊最新文献
Non-Surgical Management of Recurrent Naso-Orbital Hemangiomas with Bevacizumab: A Case Report. Safety and Efficacy of Long-Term Tocilizumab in a Cohort of Patients with Giant Cell Arteritis: An Italian Monocentric Retrospective Study. Exploring Anticancer Potential of Lactobacillus Strains: Insights into Cytotoxicity and Apoptotic Mechanisms on HCT 115 Cancer Cells. Silencing AREG Enhances Sensitivity to Irradiation by Suppressing the PI3K/AKT Signaling Pathway in Colorectal Cancer Cells. Preliminary Investigation and Therapeutic Efficacy Determination of a Novel Anti-IL-17A Antibody, Indikizumab.
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