Paula Meneghetti, Mariana Ottaiano Gonçalves, Gabriela Villa Marin, Juliana Fortes Di Iorio, Náthani Gabrielly Silva Negreiros, Ana Claudia Torrecilhas
{"title":"Extracellular vesicles: Methods for purification and characterization.","authors":"Paula Meneghetti, Mariana Ottaiano Gonçalves, Gabriela Villa Marin, Juliana Fortes Di Iorio, Náthani Gabrielly Silva Negreiros, Ana Claudia Torrecilhas","doi":"10.1016/bs.ctm.2024.06.005","DOIUrl":null,"url":null,"abstract":"<p><p>Extracellular vesicles (EVs) are membrane-bound particles released by cells that play a significant role in intercellular communication. They can be obtained from a variety of sources, including conditioned culture medium, blood and urine. In this chapter we detail the methods for EV isolation and characterization. Isolating and characterizing EVs is essential for understanding their functions in physiological and pathological processes. Advances in isolation and characterization techniques provide opportunities for deeper research into EV biology and its potential applications in diagnostics and therapeutics.</p>","PeriodicalId":11029,"journal":{"name":"Current topics in membranes","volume":"94 ","pages":"33-48"},"PeriodicalIF":0.0000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current topics in membranes","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1016/bs.ctm.2024.06.005","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/8/23 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
引用次数: 0
Abstract
Extracellular vesicles (EVs) are membrane-bound particles released by cells that play a significant role in intercellular communication. They can be obtained from a variety of sources, including conditioned culture medium, blood and urine. In this chapter we detail the methods for EV isolation and characterization. Isolating and characterizing EVs is essential for understanding their functions in physiological and pathological processes. Advances in isolation and characterization techniques provide opportunities for deeper research into EV biology and its potential applications in diagnostics and therapeutics.
细胞外囊泡（EVs）是细胞释放的膜结合颗粒，在细胞间通信中发挥着重要作用。它们可从多种来源获得，包括条件培养基、血液和尿液。本章将详细介绍 EVs 的分离和表征方法。分离和表征 EV 对于了解它们在生理和病理过程中的功能至关重要。分离和表征技术的进步为深入研究 EV 生物学及其在诊断和治疗中的潜在应用提供了机会。
期刊介绍：
Current Topics in Membranes provides a systematic, comprehensive, and rigorous approach to specific topics relevant to the study of cellular membranes. Each volume is a guest edited compendium of membrane biology.
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