INVESTIGATION THE EXPRESSION LEVELS OF MIR-181 AND HOXA11 GENE IN EUTOPIC AND ECTOPIC ENDOMETRIAL TISSUE.

IF 0.7 4区 医学 Q4 ENDOCRINOLOGY & METABOLISM Acta Endocrinologica-Bucharest Pub Date : 2024-01-01 Epub Date: 2024-10-03 DOI:10.4183/aeb.2024.33
S A Miraboutalebi, M Dehghani Ashkezari, S M Seifati
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Abstract

Objectives: The exact pathogenesis of the endometriosis is not apparent. MicroRNAs (miRNAs/miRs) are non-coding RNAs that regulate gene expression at the post-transcriptional level. MicroRNAs can be used a diagnostic and therapeutic tools in different disorders such as endometriosis. MiR-181 has a function in embryo implantation. The main aim of this study is to evaluate the expression of miR-181 and its relationship with HOXA11 gene expression in ectopic and eutopic endometrium tissues in women with endometriosis.

Study design: Thirty-four women participated in this study. Ectopic tissue samples (N=17) were collected via laparoscopic surgery, and eutopic tissue samples (N=17) were obtained from an endometrial biopsy. Endometrial tissue samples without endometriosis were considered the control group. Tissue samples were placed in RNase-free microtube with RNAlater™ Stabilization Solution (Thermo Fisher Scientific) and were kept at -80 °C. Quantitative real time-PCR for MiR-181 and HOXA11 genes were performed.

Results: MiR-181 expression level increased in eutopic tissue samples compared to the control group. This expression showed a significantly decrease in an ectopic group compared to the eutopic group. It was observed that HOXA11expression decreased remarkably in eutopic group compared to the control group and increased in ectopic group compared to the eutopic group.

Conclusion: MiR-181 and HOXA11 are promising strategies in endometriosis disease. Understanding this relation and regulation roles contribute to realizing the etiology of endometriosis.

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研究 mir-181 和 hoxa11 基因在异位和异位子宫内膜组织中的表达水平。
目的:子宫内膜异位症的确切发病机制尚不清楚。微小核糖核酸(miRNA/miRs)是一种非编码核糖核酸,可在转录后水平调节基因表达。微RNA可作为子宫内膜异位症等不同疾病的诊断和治疗工具。MiR-181 在胚胎植入中具有一定的功能。本研究的主要目的是评估子宫内膜异位症妇女异位和异位子宫内膜组织中 miR-181 的表达及其与 HOXA11 基因表达的关系:研究设计:34名妇女参与了本研究。异位组织样本(17 例)通过腹腔镜手术采集,异位组织样本(17 例)通过子宫内膜活检获得。无子宫内膜异位症的子宫内膜组织样本被视为对照组。组织样本被置于装有 RNAlater™ 稳定液(赛默飞世尔科技公司)的无 RNase 微管中,并保存在 -80 °C。对 MiR-181 和 HOXA11 基因进行实时定量 PCR 检测:结果:与对照组相比,异位组织样本中 MiR-181 的表达水平升高。与异位组相比,异位组的 MiR-181 表达水平明显下降。与对照组相比,异位组的HOXA11表达量明显下降,而异位组的HOXA11表达量则比异位组有所增加:结论:MiR-181和HOXA11是治疗子宫内膜异位症的有效策略。结论:MiR-181和HOXA11在子宫内膜异位症的研究中具有广阔的前景,了解它们的关系和调控作用有助于了解子宫内膜异位症的病因。
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来源期刊
Acta Endocrinologica-Bucharest
Acta Endocrinologica-Bucharest 医学-内分泌学与代谢
CiteScore
1.30
自引率
20.00%
发文量
53
审稿时长
6-12 weeks
期刊介绍: Acta Endocrinologica (Buc) is an international journal covering the fields of basic and clinical Endocrinology, Neuroendocrinology, Reproductive Medicine, Chronobiology, Human Ethology published quarterly Acta Endocrinologica (Buc) is the official international journal of the Romanian Society for Endocrinology. It continues the former Romanian Journal of Endocrinology
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