Hollow microporous organic network fiber membrane for efficient extraction of okadaic acid from marine organisms

IF 3.8 2区 化学 Q1 BIOCHEMICAL RESEARCH METHODS Journal of Chromatography A Pub Date : 2024-10-05 DOI:10.1016/j.chroma.2024.465392
Wenmin Zhang , Baodi Liao , Shiye Xie , Lan Zhang
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Abstract

Membrane-based micro-solid phase extraction (M-μSPE) has garnered great attention in sample pretreatment, suffering an inherent contradiction between permeability and adsorption capacity. In this study, a pure microporous organic network (TEB-DIB-MON) fiber membrane was prepared by combining electrostatic spinning technology, Sonogashira-Hagihara reaction and template sacrifice method. The prepared TEB-DIB-MON membrane exhibited a large specific surface area with a hollow and porous structure, thereby providing excellent solvent permeability and high adsorption capacity for okadaic acid (OA, an algal toxin). Under the optimized conditions, a sensitive analytical method was established by coupling M-μSPE with high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The established method has a low detection limit (0.5 pg mL−1), a wide linear range (1.5–1000 pg mL−1, R ≥ 0.9991), and good reproducibility (RSD ≤ 9.4 %, n = 6), which was then successfully applied for OA detection in marine organisms. Trace amounts of OA (59.3–89.0 pg mL−1) was detected in the oyster and prawn samples. This work demonstrated that the excellent application potential of MON membranes in sample pretreatment, while also presents a novel synthesis strategy for MONs membranes.
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用于从海洋生物中高效提取冈田酸的中空微孔有机网络纤维膜。
基于膜的微固相萃取(M-μSPE)在样品预处理中备受关注,但其渗透性与吸附能力之间存在固有的矛盾。本研究结合静电纺丝技术、Sonogashira-Hagihara 反应和模板牺牲法制备了纯微孔有机网络(TEB-DIB-MON)纤维膜。所制备的 TEB-DIB-MON 膜具有较大的比表面积和中空多孔结构,因而具有优异的溶剂渗透性和较高的 okadaic 酸(OA,一种藻类毒素)吸附能力。在优化的条件下,通过将 M-μSPE 与高效液相色谱-串联质谱(HPLC-MS/MS)联用,建立了灵敏的分析方法。该方法检出限低(0.5 pg mL-1),线性范围宽(1.5-1000 pg mL-1,R ≥ 0.9991),重现性好(RSD ≤ 9.4 %,n = 6),可成功用于海洋生物中 OA 的检测。在牡蛎和对虾样品中检测到了微量的 OA(59.3-89.0 pg mL-1)。这项工作证明了 MON 膜在样品预处理方面的巨大应用潜力,同时也提出了一种新的 MON 膜合成策略。
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来源期刊
Journal of Chromatography A
Journal of Chromatography A 化学-分析化学
CiteScore
7.90
自引率
14.60%
发文量
742
审稿时长
45 days
期刊介绍: The Journal of Chromatography A provides a forum for the publication of original research and critical reviews on all aspects of fundamental and applied separation science. The scope of the journal includes chromatography and related techniques, electromigration techniques (e.g. electrophoresis, electrochromatography), hyphenated and other multi-dimensional techniques, sample preparation, and detection methods such as mass spectrometry. Contributions consist mainly of research papers dealing with the theory of separation methods, instrumental developments and analytical and preparative applications of general interest.
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