Ultraviolet (UV-C) Light Systems for the Inactivation of Feline Calicivirus and Tulane Virus in Model Fluid Foods

IF 4.1 2区农林科学 Q2 ENVIRONMENTAL SCIENCES Food and Environmental Virology Pub Date : 2024-10-10 DOI:10.1007/s12560-024-09614-2

E. Corson, B. Pendyala, A. Patras, D. H. D’Souza

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Abstract

Conventional UV-C (254 nm) inactivation technologies have limitations and potential operator-safety risk. To overcome these disadvantages, novel UV-C light-emitting diodes (LED) are developed and investigated for their performance. This study aimed to determine the inactivation of human norovirus (HuNoV) surrogates, Tulane virus (TV), and feline calicivirus (FCV-F9), by UV-C (254 nm) in comparison to UV-C LED (279 nm) in phosphate-buffered saline (PBS) and coconut water (CW). Five-hundred microliters of FCV-F9 (~ 5 log plaque forming units (PFU)/mL) or TV (~ 6 log PFU/mL) were added to 4.5 mL PBS or CW in continuously stirred glass beakers and exposed to 254 nm UV-C for 0 up to 15 min (maximum dosage of 33.89 mJ/cm²) or 279 nm UV-C LED for 0 up to 2.5 min (maximum dosage of 7.03 mJ/cm²). Recovered viruses were assayed in duplicate from each treatment replicated thrice. Mixed model analysis of variance was used for data analysis. Significantly lower D₁₀ values were obtained in PBS and CW (p ≤ 0.05) for both tested viruses using UV-C LED (279 nm) where FCV-F9 showed D₁₀ values of 7.08 ± 1.75 mJ/cm² and 3.75 ± 0.11 mJ/cm², while using UV-C (254 nm) showed D₁₀ values of 13.81 ± 0.40 mJ/cm² and 6.43 ± 0.44 mJ/cm² in PBS and CW, respectively. Similarly, lower D₁₀ values were obtained for TV of 3.91 ± 1.03 mJ/cm² and 4.26 ± 1.02 mJ/cm² with 279 nm UV-C LED and were 18.76 ± 3.16 mJ/cm² and 10.21 ± 1.48 mJ/cm² with 254 nm UV-C in PBS and CW, respectively. Viral resistance to these treatments was fluid-matrix dependent. These findings indicate that use of 279 nm UV-C LED is more effective in inactivating HuNoV surrogates than conventional 254 nm UV-C in the tested fluids.

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紫外线 (UV-C) 光系统用于灭活模型流体食品中的猫钙病毒和杜兰病毒。

传统的紫外线-C（254 纳米）灭活技术有其局限性和潜在的操作安全风险。为了克服这些缺点，开发了新型 UV-C 发光二极管 (LED)，并对其性能进行了研究。本研究旨在确定紫外线-C（254 纳米）与紫外线-C 发光二极管（279 纳米）在磷酸盐缓冲盐水（PBS）和椰子水（CW）中对人类诺瓦克病毒（HuNoV）代用品、杜兰病毒（TV）和猫犊牛病毒（FCV-F9）的灭活效果比较。将 500 微升 FCV-F9（约 5 log plaque forming units (PFU)/mL ）或 TV（约 6 log PFU/mL）加入 4.5 mL 磷酸盐缓冲液或椰子水中，在连续搅拌的玻璃烧杯中，暴露于 254 纳米 UV-C 下 0 至 15 分钟（最大剂量为 33.89 mJ/cm2），或 279 纳米 UV-C LED 下 0 至 2.5 分钟（最大剂量为 7.03 mJ/cm2）。回收的病毒一式两份，每种处理重复三次。数据分析采用混合方差分析模型。使用 UV-C LED (279 nm) 时，两种测试病毒在 PBS 和 CW 中的 D10 值均显著降低（p ≤ 0.05），其中 FCV-F9 的 D10 值分别为 7.08 ± 1.75 mJ/cm2 和 3.75 ± 0.11 mJ/cm2，而使用 UV-C (254 nm) 时，PBS 和 CW 中的 D10 值分别为 13.81 ± 0.40 mJ/cm2 和 6.43 ± 0.44 mJ/cm2。同样，在 279 纳米 UV-C LED 中，TV 的 D10 值较低，分别为 3.91 ± 1.03 mJ/cm2 和 4.26 ± 1.02 mJ/cm2；在 PBS 和 CW 中，254 纳米 UV-C 的 D10 值较低，分别为 18.76 ± 3.16 mJ/cm2 和 10.21 ± 1.48 mJ/cm2。病毒对这些处理方法的耐受性取决于流体基质。这些研究结果表明，在测试液体中使用 279 纳米 UV-C LED 比传统的 254 纳米 UV-C 能更有效地灭活 HuNoV 代用品。

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来源期刊

Food and Environmental Virology ENVIRONMENTAL SCIENCES-MICROBIOLOGY

CiteScore

6.50

自引率

2.90%

发文量

审稿时长

1 months

期刊介绍： Food and Environmental Virology publishes original articles, notes and review articles on any aspect relating to the transmission of pathogenic viruses via the environment (water, air, soil etc.) and foods. This includes epidemiological studies, identification of novel or emerging pathogens, methods of analysis or characterisation, studies on survival and elimination, and development of procedural controls for industrial processes, e.g. HACCP plans. The journal will cover all aspects of this important area, and encompass studies on any human, animal, and plant pathogenic virus which is capable of transmission via the environment or food.