Characterization of neurologic disease-associated Streptococcus suis strains within the United States swine herd and use of diagnostic tools.

IF 6.1 2区 医学 Q1 MICROBIOLOGY Journal of Clinical Microbiology Pub Date : 2024-11-13 Epub Date: 2024-10-08 DOI:10.1128/jcm.00374-24
Jessica Santos Streauslin, Daniel W Nielsen, Kent J Schwartz, Rachel J Derscheid, Drew R Magstadt, Eric R Burrough, Phillip C Gauger, Loni L Schumacher, Michael C Rahe, Alyona Michael, Panchan Sitthicharoenchai, Christopher L Siepker, Franco Matias Ferreyra, Marcelo Nunes de Almeida, Rodger Main, Laura K Bradner, Xiao Hu, Ganwu Li, Ana Paula S Poeta Silva, Orhan Sahin, Bailey L Arruda
{"title":"Characterization of neurologic disease-associated <i>Streptococcus suis</i> strains within the United States swine herd and use of diagnostic tools.","authors":"Jessica Santos Streauslin, Daniel W Nielsen, Kent J Schwartz, Rachel J Derscheid, Drew R Magstadt, Eric R Burrough, Phillip C Gauger, Loni L Schumacher, Michael C Rahe, Alyona Michael, Panchan Sitthicharoenchai, Christopher L Siepker, Franco Matias Ferreyra, Marcelo Nunes de Almeida, Rodger Main, Laura K Bradner, Xiao Hu, Ganwu Li, Ana Paula S Poeta Silva, Orhan Sahin, Bailey L Arruda","doi":"10.1128/jcm.00374-24","DOIUrl":null,"url":null,"abstract":"<p><p><i>Streptococcus suis</i> negatively impacts swine health, posing diagnostic and preventative challenges. <i>S. suis</i> can induce disease and also quietly reside on mucosal surfaces. The limited use of diagnostic tools to identify disease-associated strains and rule out differential diagnoses, alongside the complex ecology of <i>S. suis</i>, poses significant challenges in comprehending this important pathogen and defining pathotypes. This study evaluated 2,379 <i>S</i>. <i>suis</i> central nervous system (CNS) isolates from diagnostic submissions between 2015 and 2019. Isolates originating from submissions with histologic evidence of CNS infection (<i>n</i> = 1,032) were further characterized by standard and advanced diagnostic techniques. We identified 29 <i>S</i>. <i>suis</i> serotypes and 4 reclassified serotypes as putative causes of CNS disease. Among these, serotypes 1 and 7 emerged as the predominant putative causes of CNS infection (32% of submissions). Furthermore, 51 sequence types (STs), of which 15 were novel, were detected with ST1 predominating. Through whole-genome sequencing of 145 isolates, we observed that five commonly used virulence-associated genes (VAGs; <i>epf</i>, <i>mrp</i>, <i>sly</i>, <i>ofs</i>, and <i>srtF</i>) were not present in most disease-associated isolates, and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) yielded false-positive results in 7% of isolates. These data indicate that (i) clinical signs and site of isolation alone are insufficient for defining a pathotype, (ii) <i>S. suis</i> serotypes and STs associated with CNS infection are more diverse than previously reported, (iii) MALDI-TOF MS may need to be supplemented with additional diagnostic tools for precise <i>S. suis</i> identification, and (iv) VAGs remain an unreliable means for identifying isolates associated with CNS disease.IMPORTANCE<i>Streptococcus suis</i> is an important and complex systemic bacterial pathogen of swine. Characterization of <i>S. suis</i> strains originating from pigs with histologic confirmation of neurologic disease is limited. Review of swine diagnostic submissions revealed that fewer than half of cases from which <i>S. suis</i> was isolated from the brain had histologic evidence of neurologic disease. This finding demonstrates that clinical signs and site of isolation alone are not sufficient for identifying a neurologic disease-associated strain. Characterization of strains originating from cases with evidence of disease using classic and advanced diagnostic techniques revealed that neurologic disease-associated strains are diverse and commonly lack genes previously associated with virulence.</p>","PeriodicalId":15511,"journal":{"name":"Journal of Clinical Microbiology","volume":null,"pages":null},"PeriodicalIF":6.1000,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Clinical Microbiology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1128/jcm.00374-24","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/10/8 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Streptococcus suis negatively impacts swine health, posing diagnostic and preventative challenges. S. suis can induce disease and also quietly reside on mucosal surfaces. The limited use of diagnostic tools to identify disease-associated strains and rule out differential diagnoses, alongside the complex ecology of S. suis, poses significant challenges in comprehending this important pathogen and defining pathotypes. This study evaluated 2,379 S. suis central nervous system (CNS) isolates from diagnostic submissions between 2015 and 2019. Isolates originating from submissions with histologic evidence of CNS infection (n = 1,032) were further characterized by standard and advanced diagnostic techniques. We identified 29 S. suis serotypes and 4 reclassified serotypes as putative causes of CNS disease. Among these, serotypes 1 and 7 emerged as the predominant putative causes of CNS infection (32% of submissions). Furthermore, 51 sequence types (STs), of which 15 were novel, were detected with ST1 predominating. Through whole-genome sequencing of 145 isolates, we observed that five commonly used virulence-associated genes (VAGs; epf, mrp, sly, ofs, and srtF) were not present in most disease-associated isolates, and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) yielded false-positive results in 7% of isolates. These data indicate that (i) clinical signs and site of isolation alone are insufficient for defining a pathotype, (ii) S. suis serotypes and STs associated with CNS infection are more diverse than previously reported, (iii) MALDI-TOF MS may need to be supplemented with additional diagnostic tools for precise S. suis identification, and (iv) VAGs remain an unreliable means for identifying isolates associated with CNS disease.IMPORTANCEStreptococcus suis is an important and complex systemic bacterial pathogen of swine. Characterization of S. suis strains originating from pigs with histologic confirmation of neurologic disease is limited. Review of swine diagnostic submissions revealed that fewer than half of cases from which S. suis was isolated from the brain had histologic evidence of neurologic disease. This finding demonstrates that clinical signs and site of isolation alone are not sufficient for identifying a neurologic disease-associated strain. Characterization of strains originating from cases with evidence of disease using classic and advanced diagnostic techniques revealed that neurologic disease-associated strains are diverse and commonly lack genes previously associated with virulence.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
美国猪群中与神经系统疾病相关的猪链球菌菌株特征及诊断工具的使用。
猪链球菌对猪的健康有负面影响,给诊断和预防带来挑战。猪链球菌可诱发疾病,也可静静地栖息在粘膜表面。由于使用的诊断工具有限,无法确定与疾病相关的菌株和排除鉴别诊断,再加上猪链球菌的生态环境复杂,这给了解这种重要病原体和确定病原体类型带来了巨大挑战。本研究评估了 2015 年至 2019 年期间从诊断呈文中分离出的 2379 株猪链球菌中枢神经系统(CNS)分离株。通过标准和先进的诊断技术,对来自有中枢神经系统感染组织学证据(n = 1,032)的分离物进行了进一步鉴定。我们确定了 29 个猪链球菌血清型和 4 个重新分类的血清型可能是中枢神经系统疾病的病因。其中,血清型 1 和 7 成为中枢神经系统感染的主要推定病因(占提交病例的 32%)。此外,还发现了 51 种序列类型(ST),其中 15 种是新的,以 ST1 型为主。通过对 145 株分离株进行全基因组测序,我们观察到大多数与疾病相关的分离株中不存在五个常用的毒力相关基因(VAGs:epf、mrp、sly、ofs 和 srtF),基质辅助激光解吸电离飞行时间质谱法(MALDI-TOF MS)在 7% 的分离株中产生了假阳性结果。这些数据表明:(i) 仅凭临床症状和分离部位不足以确定病原体类型;(ii) 与中枢神经系统感染相关的猪链球菌血清型和 ST 比以前报道的更为多样;(iii) MALDI-TOF MS 可能需要辅以其他诊断工具才能精确鉴定猪链球菌;(iv) VAG 仍是鉴定与中枢神经系统疾病相关分离物的不可靠方法。猪链球菌是一种重要而复杂的全身性细菌病原体,对来自猪的猪链球菌菌株进行特征描述并经组织学证实患有神经系统疾病的情况非常有限。对猪诊断报告的审查显示,从脑中分离出猪链球菌的病例中,只有不到一半有神经系统疾病的组织学证据。这一发现表明,仅凭临床症状和分离部位不足以确定神经系统疾病相关菌株。使用传统和先进的诊断技术对来自有疾病证据的病例的菌株进行特征描述后发现,神经系统疾病相关菌株多种多样,通常缺乏以前与毒力相关的基因。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Journal of Clinical Microbiology
Journal of Clinical Microbiology 医学-微生物学
CiteScore
17.10
自引率
4.30%
发文量
347
审稿时长
3 months
期刊介绍: The Journal of Clinical Microbiology® disseminates the latest research concerning the laboratory diagnosis of human and animal infections, along with the laboratory's role in epidemiology and the management of infectious diseases.
期刊最新文献
Evaluation of gradient strip diffusion for susceptibility testing of aztreonam-avibactam in metallo-β-lactamase-producing Enterobacterales. Whole-genome sequencing resolves biochemical misidentification of Neisseria species from urogenital specimens. Oxford Nanopore's 2024 sequencing technology for Listeria monocytogenes outbreak detection and source attribution: progress and clone-specific challenges. Persistent bloodstream infection in children: examining the role for repeat blood cultures. Characterization of neurologic disease-associated Streptococcus suis strains within the United States swine herd and use of diagnostic tools.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1