CRISPR-Cas9: Unraveling Genetic Secrets to Enhance Floral and Fruit Traits in Tomato.

IF 2.4 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Molecular Biotechnology Pub Date : 2024-10-08 DOI:10.1007/s12033-024-01290-8

S Bhoomika, Shubham Rajaram Shalunkhe, A R Sakthi, T Saraswathi, S Manonmani, M Raveendran, M Sudha

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Abstract

Tomato, a globally consumed vegetable, possesses vast genetic diversity, making it suitable for genetic manipulation using various genetic improvement techniques. Tomatoes are grown extensively for their market value and health benefits, primarily contributed by enhanced yield and nutritional value respectively, influenced by floral and fruit traits. Floral morphology is maintained by genes involved in meristem size control, regulation of inflorescence transition, and pollen development. SP (SELF-PRUNING) and SP5G (SELF-PRUNING 5G) determine growth habit and flowering time. RIN (RIPENING INHIBITOR) and PG (POLYGALACTURONASE) are responsible for the shelf life of fruits. In addition to this, nutrition-enriched tomatoes have been developed in recent times. In this review, we comprehensively discuss the major genes influencing floral morphology, flowering time, fruit size, fruit shape, shelf life, and nutritional value, ultimately resulting in enhanced yield. Additionally, we address the advances in CRISPR/Cas9 applied for the genetic improvement of tomatoes along with prospects of areas in which research development in terms of tomato genetic improvement has to be advanced.

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CRISPR-Cas9：揭开增强番茄花果性状的遗传秘密。

番茄是一种全球消费的蔬菜，具有广泛的遗传多样性，适合利用各种遗传改良技术进行遗传操作。番茄因其市场价值和对健康的益处而被广泛种植，其主要原因是受花和果实性状的影响，产量和营养价值分别有所提高。花的形态由参与分生组织大小控制、花序过渡调控和花粉发育的基因维持。SP（SELF-PRUNING）和 SP5G（SELF-PRUNING 5G）决定了生长习性和开花时间。RIN（裂解抑制剂）和 PG（多聚半乳糖醛酸酶）负责水果的货架期。除此之外，近来还开发出了富含营养的番茄。在本综述中，我们全面讨论了影响花形态、开花时间、果实大小、果实形状、货架期和营养价值的主要基因，这些基因最终会提高产量。此外，我们还讨论了应用 CRISPR/Cas9 进行番茄遗传改良的进展，以及番茄遗传改良研究发展的前景。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular Biotechnology 医学-生化与分子生物学

CiteScore

4.10

自引率

3.80%

发文量

165

审稿时长

6 months

期刊介绍： Molecular Biotechnology publishes original research papers on the application of molecular biology to both basic and applied research in the field of biotechnology. Particular areas of interest include the following: stability and expression of cloned gene products, cell transformation, gene cloning systems and the production of recombinant proteins, protein purification and analysis, transgenic species, developmental biology, mutation analysis, the applications of DNA fingerprinting, RNA interference, and PCR technology, microarray technology, proteomics, mass spectrometry, bioinformatics, plant molecular biology, microbial genetics, gene probes and the diagnosis of disease, pharmaceutical and health care products, therapeutic agents, vaccines, gene targeting, gene therapy, stem cell technology and tissue engineering, antisense technology, protein engineering and enzyme technology, monoclonal antibodies, glycobiology and glycomics, and agricultural biotechnology.