Evaluation of 4 quantification methods for monitoring 16 antidepressant drugs and their metabolites in human plasma by LC-MS/MS

IF 1.3 4区 医学 Q4 PHARMACOLOGY & PHARMACY Journal of pharmacological and toxicological methods Pub Date : 2024-10-09 DOI:10.1016/j.vascn.2024.107568
Lingjiao Wang , Mengqiang Zhao , Jiao Li , Hongtao Xu , Yang Song , Yuanyuan Zhao , Jing Yu , Chunhua Zhou
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Abstract

Background

Therapeutic drug monitoring for antidepressants (ADs) is vital due to the potentially serious consequences and disputes related to medical events. Therefore, we created a quick and convenient analysis way for separation and quantification of ADs.

Methods

To ensure quantitative stability, we divided the 16 ADs or their metabolites into 4 pools (AD1-AD4), considering the hospital frequency that the clinician prescribed, the physicochemical properties of medicines, and the calibration range of selected ADs. After precipitation with methanol, the analytes were eluted for at least 3.5 min on a BEH C18 analytical column by different gradient elution methods. Results: The LLOQ and LOD were 1.25–10 ng/mL and 0.42–5 ng/mL, respectively. High precision (<12 %) and accuracy (87.07–111.47 %) were demonstrated by quality control samples both within and between days. All the compounds were stable at room temperature and within −80 °C.

Conclusion

The method is of wide clinical and laboratory interest due to simpler sample cleanup, shorter chromatographic run times, and wider calibration range compared to other methods.
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评估用 LC-MS/MS 监测人体血浆中 16 种抗抑郁药物及其代谢物的 4 种定量方法。
背景:抗抑郁药(ADs)的治疗药物监测至关重要,因为它可能造成严重后果,并引发医疗纠纷。因此,我们创建了一种快速、便捷的分析方法来分离和量化抗抑郁药:为了确保定量的稳定性,我们根据临床医生开具处方的医院频率、药物的理化性质以及所选 ADs 的校准范围,将 16 种 ADs 或其代谢物分为 4 个池(AD1-AD4)。分析物经甲醇沉淀后,在 BEH C18 分析柱上用不同的梯度洗脱方法洗脱至少 3.5 分钟:结果:LLOQ 和 LOD 分别为 1.25-10 纳克/毫升和 0.42-5 纳克/毫升。结果:LLOQ 和 LOD 分别为 1.25-10 ng/mL 和 0.42-5 ng/mL:与其他方法相比,该方法样品清理简单、色谱运行时间短、校准范围广,因此受到临床和实验室的广泛关注。
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来源期刊
Journal of pharmacological and toxicological methods
Journal of pharmacological and toxicological methods PHARMACOLOGY & PHARMACY-TOXICOLOGY
CiteScore
3.60
自引率
10.50%
发文量
56
审稿时长
26 days
期刊介绍: Journal of Pharmacological and Toxicological Methods publishes original articles on current methods of investigation used in pharmacology and toxicology. Pharmacology and toxicology are defined in the broadest sense, referring to actions of drugs and chemicals on all living systems. With its international editorial board and noted contributors, Journal of Pharmacological and Toxicological Methods is the leading journal devoted exclusively to experimental procedures used by pharmacologists and toxicologists.
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