The Relationship Between Serum IgE Level and IL-4 and IL-13 Cytokines in Colorectal Cancer Patients.

IF 2.9 4区医学 Q3 IMMUNOLOGY Immunological Investigations Pub Date : 2024-10-11 DOI:10.1080/08820139.2024.2414091

Zahra Mozooni, Fatemeh Faraji, Sara Minaeian, Leyla Bahadorizadeh

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Abstract

Background: Colorectal cancer (CRC) is the most common malignancy of the digestive system in the world. Immune cells and molecules in tumor microenvironment are crucial.Identifying immune system components in cancer aids in biomarker discovery. This study investigated the serum IgE levels and expression of IL-4 and IL-13 in the tissue and serum of CRC patients and explored their possible association with pathological and clinical factors.

Materials and methods: Thirty-six patients with CRC and 36 healthy individuals were involved in the study. Tissues and blood samples were collected. Serum levels of IgE and IL-4 and IL-13 were analyzed using the ELISA method. The quantitative Real-Time PCR (qRT-PCR) technique was used to assess the expression levels of the cytokines in CRC tissue samples in comparison with the adjacent control tissue.

Results: Our results revealed that the serum level of IL-4 and IL-13 and also their gene expression levels were significantly decreased in CRC patients compared to the controls. The results of this study revealed that there is no significant difference in the serum levels of IgE between CRC patients and the control group.

Conclusion: All in all, the results of the current research suggest that the expression levels of IL-13, IL-4, and IgE vary between CRC tissue.

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结直肠癌患者血清 IgE 水平与 IL-4 和 IL-13 细胞因子之间的关系

背景：结直肠癌（CRC）是世界上最常见的消化系统恶性肿瘤。肿瘤微环境中的免疫细胞和分子至关重要，识别癌症中的免疫系统成分有助于生物标记物的发现。本研究调查了 CRC 患者血清 IgE 水平以及组织和血清中 IL-4 和 IL-13 的表达，并探讨了它们与病理和临床因素的可能关联：研究涉及 36 名 CRC 患者和 36 名健康人。收集组织和血液样本。采用 ELISA 方法分析血清中 IgE、IL-4 和 IL-13 的水平。采用定量实时 PCR（qRT-PCR）技术评估细胞因子在 CRC 组织样本中的表达水平，并与邻近的对照组织进行比较：结果：我们的研究结果表明，与对照组相比，CRC 患者血清中 IL-4 和 IL-13 的水平及其基因表达水平明显下降。本研究结果显示，CRC 患者与对照组的血清 IgE 水平无明显差异：总之，本次研究结果表明，IL-13、IL-4 和 IgE 的表达水平在不同的 CRC 组织中存在差异。

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来源期刊

Immunological Investigations 医学-免疫学

CiteScore

5.50

自引率

7.10%

发文量

审稿时长

3 months

期刊介绍： Disseminating immunological developments on a worldwide basis, Immunological Investigations encompasses all facets of fundamental and applied immunology, including immunohematology and the study of allergies. This journal provides information presented in the form of original research articles and book reviews, giving a truly in-depth examination of the latest advances in molecular and cellular immunology.