{"title":"Effects of immunorelated gene polymorphisms on trastuzumab targeting breast cancer cell <i>in vitro</i>.","authors":"Linyu Yu, Congmin Zhang, Liangyu Liu, Xiaoping Chen","doi":"10.1080/14622416.2024.2404819","DOIUrl":null,"url":null,"abstract":"<p><p><b>Aim:</b> To investigate the associations between genetic polymorphisms in immunorelated genes and PBMC-induced cytotoxicity to breast cancer cell with the treatment of trastuzumab <i>in vitro</i>.<b>Methods:</b> Trastuzumab-mediated cytotoxicity of peripheral blood mononuclear cells (PBMC) from 148 healthy donors and 13 BC patients was analyzed by flow cytometry. 16 SNPs in 7 immunorelated genes were genotyped by Sequenom Mass Array Genotype Platform.<b>Results:</b> Cytotoxicity in the trastuzumab treated PBMCs were significantly higher than those of the basal group. A wide variability in trastuzumab-mediated cytotoxicity was observed, and PBMC from individuals with the <i>CD247</i> rs16859030 T genotype generated increased cytotoxicity than those with the CC genotype.<b>Conclusion:</b> The <i>CD247</i> rs16859030 polymorphism affects trastuzumab-mediated cytotoxicity <i>in vitro</i>.</p>","PeriodicalId":20018,"journal":{"name":"Pharmacogenomics","volume":" ","pages":"461-468"},"PeriodicalIF":1.9000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11492633/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pharmacogenomics","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/14622416.2024.2404819","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/10/11 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"PHARMACOLOGY & PHARMACY","Score":null,"Total":0}
引用次数: 0
Abstract
Aim: To investigate the associations between genetic polymorphisms in immunorelated genes and PBMC-induced cytotoxicity to breast cancer cell with the treatment of trastuzumab in vitro.Methods: Trastuzumab-mediated cytotoxicity of peripheral blood mononuclear cells (PBMC) from 148 healthy donors and 13 BC patients was analyzed by flow cytometry. 16 SNPs in 7 immunorelated genes were genotyped by Sequenom Mass Array Genotype Platform.Results: Cytotoxicity in the trastuzumab treated PBMCs were significantly higher than those of the basal group. A wide variability in trastuzumab-mediated cytotoxicity was observed, and PBMC from individuals with the CD247 rs16859030 T genotype generated increased cytotoxicity than those with the CC genotype.Conclusion: The CD247 rs16859030 polymorphism affects trastuzumab-mediated cytotoxicity in vitro.
目的：研究免疫抑制基因的遗传多态性与体外使用曲妥珠单抗治疗时外周血单核细胞诱导的乳腺癌细胞毒性之间的关系：方法： 通过流式细胞术分析了148名健康供体和13名BC患者的外周血单核细胞（PBMC）在曲妥珠单抗介导下的细胞毒性。通过 Sequenom Mass Array 基因分型平台对 7 个免疫相关基因中的 16 个 SNP 进行了基因分型：结果：曲妥珠单抗治疗组的细胞毒性明显高于基础治疗组。观察到曲妥珠单抗介导的细胞毒性存在很大差异，CD247 rs16859030 T 基因型个体的 PBMC 产生的细胞毒性高于 CC 基因型个体：结论：CD247 rs16859030多态性会影响体外曲妥珠单抗介导的细胞毒性。
期刊介绍：
Pharmacogenomics (ISSN 1462-2416) is a peer-reviewed journal presenting reviews and reports by the researchers and decision-makers closely involved in this rapidly developing area. Key objectives are to provide the community with an essential resource for keeping abreast of the latest developments in all areas of this exciting field.
Pharmacogenomics is the leading source of commentary and analysis, bringing you the highest quality expert analyses from corporate and academic opinion leaders in the field.
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