{"title":"First report of southern root-knot nematode, Meloidogyne incognita, infecting papaya in Florida, USA","authors":"Abolfazl Hajihassani, Denis Gitonga, Rami Kassam","doi":"10.1007/s13314-024-00547-0","DOIUrl":null,"url":null,"abstract":"<div><p>Papaya trees showing disease symptoms caused by root-knot nematodes (<i>Meloidogyne</i> spp.) were detected in a tropical fruit farm in Vero Beach, FL, USA. Roots were severely galled containing a high population density of root-knot nematode eggs. The nematode species causing damage was identified as <i>M. incognita</i> based on the morphometrics of body, stylet, and tail length of the second-stage juveniles, and morphological characteristics of female perineal patterns. Molecular analyses using both species-specific and universal primers also confirmed the species identity. A pathogenicity test confirmed <i>M. incognita</i> reproduction on papaya by producing nematode females and egg masses within the root galls. This report documents the first confirmed detection of <i>M. incognita</i> in papaya in Florida, USA, providing detailed information on the nematode's morphology characteristics and DNA sequence data.</p></div>","PeriodicalId":45862,"journal":{"name":"Australasian Plant Disease Notes","volume":"19 1","pages":""},"PeriodicalIF":0.6000,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Australasian Plant Disease Notes","FirstCategoryId":"1085","ListUrlMain":"https://link.springer.com/article/10.1007/s13314-024-00547-0","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Papaya trees showing disease symptoms caused by root-knot nematodes (Meloidogyne spp.) were detected in a tropical fruit farm in Vero Beach, FL, USA. Roots were severely galled containing a high population density of root-knot nematode eggs. The nematode species causing damage was identified as M. incognita based on the morphometrics of body, stylet, and tail length of the second-stage juveniles, and morphological characteristics of female perineal patterns. Molecular analyses using both species-specific and universal primers also confirmed the species identity. A pathogenicity test confirmed M. incognita reproduction on papaya by producing nematode females and egg masses within the root galls. This report documents the first confirmed detection of M. incognita in papaya in Florida, USA, providing detailed information on the nematode's morphology characteristics and DNA sequence data.
在美国佛罗里达州维罗海滩的一个热带水果农场，发现木瓜树出现了由根结线虫（Meloidogyne spp.）引起的病害症状。根部严重溃烂，含有大量根结线虫卵。根据第二阶段幼虫的体长、花柱和尾长的形态计量学特征以及雌虫会阴部花纹的形态特征，确定造成损害的线虫种类为 M. incognita。使用物种特异性引物和通用引物进行的分子分析也证实了物种身份。致病性试验证实，M. incognita 在木瓜上的繁殖方式是在根部虫瘿中产生线虫雌虫和卵块。本报告首次证实在美国佛罗里达州的木瓜上发现了 M. incognita，并提供了线虫形态特征的详细信息和 DNA 序列数据。
期刊介绍：
Australasian Plant Disease Notes is an international journal for publication of short reports from all countries which describe original research that transcends national boundaries. All aspects of plant pathology are covered including new geographical records of diseases or pathogens, new pathogens or pathogen strains, taxonomic papers, quarantine notes, disease management and diagnostic methods. Australasian Plant Disease Notes is a publication of the Australasian Plant Pathology Society.
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