Tomato brown rugose fruit virus associated with leaf mosaic, mottling and brown rugose patches on fruits of tomato in India

IF 0.6 Q4 PLANT SCIENCES Australasian Plant Disease Notes Pub Date : 2024-03-08 DOI:10.1007/s13314-024-00534-5

S. S. Kavya, V. Mahantesha, A. Chowdappa, M. Mantesh, P. S. Pooja, V. Venkataravanappa, C. N. Lakshminarayana Reddy

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Abstract

Eleven fruit samples from the tomato plants exhibiting different kinds of symptoms on leaves and fruits were collected from farmers fields located in two states (Karnataka and Maharashtra) of India. To confirm the identity of the pathogen, eleven tomato fruit samples were subjected DAS-ELISA, which revealed that all samples showed positive reaction to ToBRFV antibodies. Further, total RNA was isolated from infected fruits and subjected to RT-PCR assay using tomato brown rugose fruit virus (ToBRFV) coat protein gene specific primers. The resulting PCR amplicons of ~ 500 bp from eleven tomato samples were cloned, and sequenced. Sequence analysis of the CP gene from eleven isolates showed nucleotide identity of 100% with several ToBRFV isolates reported from different countries around the world. This result was further confirmed through phylogenetic analysis, wherein the eleven ToBRFV isolates are clustered closely with isolates reported from different parts of the world. To the best of our knowledge, this is the first record of ToBRFV infecting tomato in India.

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番茄褐皱果实病毒与印度番茄果实上的叶片马赛克、斑点和褐皱斑块有关

从印度两个邦（卡纳塔克邦和马哈拉施特拉邦）的农田中采集了 11 个番茄果实样本，这些番茄植株的叶片和果实上表现出不同的症状。为了确认病原体的身份，对 11 个番茄果实样本进行了 DAS-ELISA 检测，结果显示所有样本的 ToBRFV 抗体均呈阳性反应。此外，还从受感染的果实中分离出总 RNA，并使用番茄褐皱纹果实病毒（ToBRFV）衣壳蛋白基因特异性引物进行 RT-PCR 检测。从 11 个番茄样本中得到的约 500 bp 的 PCR 扩增子被克隆和测序。对 11 个分离物的 CP 基因进行的序列分析表明，这些分离物与世界上不同国家报告的几个 ToBRFV 分离物的核苷酸同一性达到 100%。系统进化分析进一步证实了这一结果，即 11 个 ToBRFV 分离物与世界各地报告的分离物紧密聚类。据我们所知，这是印度首次记录到 ToBRFV 感染番茄。

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来源期刊

Australasian Plant Disease Notes PLANT SCIENCES-

CiteScore

1.90

自引率

0.00%

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期刊介绍： Australasian Plant Disease Notes is an international journal for publication of short reports from all countries which describe original research that transcends national boundaries. All aspects of plant pathology are covered including new geographical records of diseases or pathogens, new pathogens or pathogen strains, taxonomic papers, quarantine notes, disease management and diagnostic methods. Australasian Plant Disease Notes is a publication of the Australasian Plant Pathology Society.