ABA-Degrading Strains of Bacteria of the Genus Pseudomonas and Their Influence on Wheat Growth

IF 1 4区生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Applied Biochemistry and Microbiology Pub Date : 2024-09-22 DOI:10.1134/S0003683824604566

A. S. Ryabova, L. Yu. Kuzmina, N. F. Galimsyanova, E. A. Gilvanova, L. B. Vysotskaya, E. V. Martynenko, G. R. Kudoyarova

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Abstract

Three new representatives of the genus Pseudomonas have been identified that are capable of utilizing abscisic acid and positively influencing the growth and development of plants. Their physiological and biochemical properties have been studied. Based on analysis of the 16S rRNA gene, they were identified as P. veronii IB K11-1 (99.86% similarity) and P. frederiksbergensis IB Ta10m (100%), and strain TaE2 was assigned to Pseudomonas sp. It was found that bacteria, when grown on a mineral-salt medium with ABA, reduced the hormone content by 50–60% with an increase in population density by two orders of magnitude. In a laboratory experiment, it was shown that the introduction of the bacterial biomass (10⁸ CFU/g of substrate) into the rhizosphere of wheat plants ten days after treatment led to a decrease in the abscisic acid content in the roots by 18–30% and an increase in plant weight by up to 30%. Thus, new strains of growth-stimulating ABA-degrading bacteria have been identified and characterized for the first time, which may be promising for the creation of biological products that increase plant resistance to biotic and abiotic stresses.

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假单胞菌属细菌的 ABA 降解菌株及其对小麦生长的影响

已发现假单胞菌属的三个新代表能够利用赤霉酸并对植物的生长和发育产生积极影响。对它们的生理和生化特性进行了研究。根据 16S rRNA 基因分析，它们被鉴定为 P. veronii IB K11-1（相似度为 99.86%）和 P. frederiksbergensis IB Ta10m（相似度为 100%），菌株 TaE2 被归类为假单胞菌属（Pseudomonas sp.）。研究发现，细菌在含有 ABA 的矿物盐培养基上生长时，激素含量降低了 50-60%，种群密度增加了两个数量级。实验室实验表明，将细菌生物量（108 CFU/克基质）引入小麦植株根瘤层十天后，根中的赤霉酸含量减少了 18-30%，植株重量增加了 30%。因此，我们首次发现了新的刺激生长的 ABA 降解菌株，并对其进行了表征，这对于创造生物产品以提高植物对生物和非生物胁迫的抵抗力很有希望。

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来源期刊

Applied Biochemistry and Microbiology 生物-生物工程与应用微生物

CiteScore

1.70

自引率

12.50%

发文量

审稿时长

6-12 weeks

期刊介绍： Applied Biochemistry and Microbiology is an international peer reviewed journal that publishes original articles on biochemistry and microbiology that have or may have practical applications. The studies include: enzymes and mechanisms of enzymatic reactions, biosynthesis of low and high molecular physiologically active compounds; the studies of their structure and properties; biogenesis and pathways of their regulation; metabolism of producers of biologically active compounds, biocatalysis in organic synthesis, applied genetics of microorganisms, applied enzymology; protein and metabolic engineering, biochemical bases of phytoimmunity, applied aspects of biochemical and immunochemical analysis; biodegradation of xenobiotics; biosensors; biomedical research (without clinical studies). Along with experimental works, the journal publishes descriptions of novel research techniques and reviews on selected topics.