An X-Ray Diffraction Study of Lipid Films with ICHPHAN

IF 4.033 Q4 Biochemistry, Genetics and Molecular Biology Biophysics Pub Date : 2024-09-27 DOI:10.1134/S0006350924700258
A. V. Krivandin, A. N. Goloshchapov
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Abstract

X-ray diffraction was used to study oriented films formed from solutions in chloroform of egg phosphatidylcholine, the synthetic hybrid antioxidant ICHPHAN-10-C-10, and their mixtures containing 12.5 and 32 wt % of ICHPHAN. The air-dried films obtained from phosphatidylcholine and ICHPHAN were multiphase and, in addition to nonlayered lipid phases, contained the crystalline phase of ICHPHAN. With an increase in the degree of hydration of such films, the formation of a single lamellar phase consisting of lipids and ICHPHAN occurred in them. The appearance and disappearance of the crystalline phase of ICHPHAN in the films with a change in their degree of hydration was reversible. The electron density profiles of lipid membranes calculated with a resolution of ~1.2 nm showed that in the presence of ICHPHAN, the membrane thickness was reduced and the ordering of lipid hydrocarbon chains in it decreased. The results we obtained indicated the ability of ICHPHAN to integrate from the crystalline phase into lipid membranes in large quantities.

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用 ICHPHAN 对脂质薄膜进行 X 射线衍射研究
利用 X 射线衍射法研究了鸡蛋磷脂酰胆碱、合成混合抗氧化剂 ICHPHAN-10-C-10 及其含有 12.5 和 32 wt % ICHPHAN 的混合物在氯仿中形成的定向薄膜。由磷脂酰胆碱和 ICHPHAN 制成的风干薄膜是多相的,除了非层状脂相外,还含有 ICHPHAN 的结晶相。随着薄膜水合程度的增加,薄膜中会形成由脂类和 ICHPHAN 组成的单层相。随着薄膜水合度的变化,薄膜中 ICHPHAN 结晶相的出现和消失是可逆的。以 ~1.2 nm 的分辨率计算的脂膜电子密度曲线显示,在 ICHPHAN 的存在下,膜的厚度减小,其中脂质碳氢链的有序性降低。我们获得的结果表明,ICHPHAN 能够从结晶相大量融入脂膜。
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来源期刊
Biophysics
Biophysics Biochemistry, Genetics and Molecular Biology-Biophysics
CiteScore
1.20
自引率
0.00%
发文量
67
期刊介绍: Biophysics is a multidisciplinary international peer reviewed journal that covers a wide scope of problems related to the main physical mechanisms of processes taking place at different organization levels in biosystems. It includes structure and dynamics of macromolecules, cells and tissues; the influence of environment; energy transformation and transfer; thermodynamics; biological motility; population dynamics and cell differentiation modeling; biomechanics and tissue rheology; nonlinear phenomena, mathematical and cybernetics modeling of complex systems; and computational biology. The journal publishes short communications devoted and review articles.
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