GC–MS quantification of fecal short-chain fatty acids and spectrophotometric detection of indole: Do rectal swabs produce comparable results as stool samples? - A pilot study

IF 1.8 3区化学 Q4 BIOCHEMICAL RESEARCH METHODS Rapid Communications in Mass Spectrometry Pub Date : 2024-10-12 DOI:10.1002/rcm.9923

Vineetha K K, Archana P R, Bhamini Krishna Rao, Mamatha Ballal, Babi Dutta, Vani Lakshmi R, Rajeshwari G. Bhat

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Abstract

Rationale

The exploration of the gut microbiome and related metabolites holds an exciting future in health science. The challenges associated with fecal sample testing are proper sample collection, sterile transportation, optimal transport conditions, and processing as all these factors could potentially change the microbiome composition, further exacerbated by the patient's customary discomfort regarding feces samples. The study aimed to compare the usage of rectal swabs and stool samples for short-chain fatty acid estimation using gas chromatography–mass spectrometry (GC–MS) and indole estimation using spectrophotometry.

Method

From May 2022 to June 2022, three women were recruited from the Department of Obstetrics and Gynecology (OBG) in a secondary care hospital in coastal Karnataka. During their clinical visit, a rectal swab was collected, and the stool sample was transported to the hospital from the patient's home in sterile containers provided. After the extraction, short-chain fatty acids (acetate, propionate, and butyrate) were quantified using GC–MS. The fecal indole concentration was determined using a hydroxylamine-based assay.

Results

The GC–MS analysis failed to detect the concentrations of short-chain fatty acids in rectal swab samples. Indole concentrations in stool and swab samples were significantly different.

Conclusion

The study's findings do not support the use of rectal swabs to analyze gut metabolites.

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粪便短链脂肪酸的气相色谱-质谱定量和吲哚的分光光度检测：直肠拭子能产生与粪便样本相似的结果吗？- 试点研究

理由探索肠道微生物组和相关代谢物在健康科学领域有着令人振奋的前景。粪便样本检测面临的挑战是样本的正确采集、无菌运输、最佳运输条件和处理，因为所有这些因素都可能改变微生物组的组成，而患者对粪便样本的习惯性不适又进一步加剧了这一挑战。本研究旨在比较直肠拭子和粪便样本在使用气相色谱-质谱法（GC-MS）估算短链脂肪酸和使用分光光度法估算吲哚含量时的使用情况。方法 2022 年 5 月至 2022 年 6 月，从卡纳塔克邦沿海地区一家二级医院的妇产科（OBG）招募了三名妇女。在临床就诊期间，采集了直肠拭子，并用提供的无菌容器将粪便样本从患者家中运送到医院。提取后，使用气相色谱-质谱对短链脂肪酸（乙酸酯、丙酸酯和丁酸酯）进行定量。粪便中的吲哚浓度是用羟胺测定法测定的。结果气相色谱-质谱分析未能检测出直肠拭子样本中的短链脂肪酸浓度。粪便和拭子样本中的吲哚浓度有显著差异。结论该研究结果不支持使用直肠拭子分析肠道代谢物。

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来源期刊

Rapid Communications in Mass Spectrometry 化学-分析化学

CiteScore

4.10

自引率

5.00%

发文量

219

审稿时长

2.6 months

期刊介绍： Rapid Communications in Mass Spectrometry is a journal whose aim is the rapid publication of original research results and ideas on all aspects of the science of gas-phase ions; it covers all the associated scientific disciplines. There is no formal limit on paper length ("rapid" is not synonymous with "brief"), but papers should be of a length that is commensurate with the importance and complexity of the results being reported. Contributions may be theoretical or practical in nature; they may deal with methods, techniques and applications, or with the interpretation of results; they may cover any area in science that depends directly on measurements made upon gaseous ions or that is associated with such measurements.