Impact of pulsed light treatment on achieving microbial safety, enzymatic stability, and shelf life extension of dragon fruit juice

Shatabdi V. Kamble, Smita S. Lele, Snehasis Chakraborty, Uday S. Annapure
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Abstract

Due to microbial and enzymatic spoilage, dragon fruit juice has a limited shelf life. This study examines the shelf life of dragon fruit juice treated with thermal and pulsed light (PL) processing and stored at 4°C. The thermal treatment at 95°C/3 min and PL treatment at 1.4 J cm−2 produce a microbially safe juice. Both thermal and PL-treated juice samples were packed in ethylene vinyl alcohol pouches and stored at refrigerated conditions (4°C). The juice's microbial, enzymatic, and biochemical attributes were analyzed over 30 days of refrigerated storage. There is no significant influence (p < 0.05) on total soluble solids, pH, and titratable acidity. The thermally treated juice loses 36% of the total phenols and 22% of antioxidants. The loss of bioactive compounds is higher in thermal than in PL treatment. The shelf life of PL-treated juice is 25 days, and thermal treatment juice is 30 days, based on the microbial analysis (6 log10 CFU mL−1). The study affirms the potential of PL treatment as an alternative technique for preserving dragon fruit juice.

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脉冲光处理对实现火龙果汁微生物安全、酶稳定性和延长保质期的影响
由于微生物和酶的腐败作用,火龙果汁的保质期有限。本研究探讨了火龙果汁经热处理和脉冲光(PL)处理后在 4°C 下储存的货架期。95°C/3分钟的热处理和1.4 J cm-2的脉冲光处理可产生微生物安全的果汁。经过热处理和脉冲光处理的果汁样品都装在乙烯-乙烯醇小袋中,并在冷藏条件下(4°C)储存。在 30 天的冷藏储存期间,对果汁的微生物、酶和生化属性进行了分析。结果表明,热处理果汁对总可溶性固形物、pH 值和可滴定酸度没有明显影响(p < 0.05)。经过热处理的果汁会损失 36% 的总酚和 22% 的抗氧化剂。生物活性化合物的损失在热处理中高于 PL 处理。根据微生物分析(6 log10 CFU mL-1),PL 处理果汁的保质期为 25 天,而热处理果汁的保质期为 30 天。这项研究肯定了聚乳酸处理作为保存火龙果汁的替代技术的潜力。
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