Real-time impedance-activated dielectrophoretic actuation for reconfigurable manipulation of single flowing particles

IF 6.1 2区工程技术 Q1 BIOCHEMICAL RESEARCH METHODS Lab on a Chip Pub Date : 2024-10-14 DOI:10.1039/d4lc00622d

Alexis Lefevre, Cristian Brandi, Adele De Ninno, Filippo Ruggiero, Enrico Verona, Michael Gauthier, Paolo Bisegna, Aude Bolopion, Federica Caselli

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Abstract

This work presents an innovative all-electrical platform for selective single-particle manipulation. The platform combines microfluidic impedance cytometry for label-free particle characterization and dielectrophoresis for contactless multi-way particle separation. The microfluidic chip has a straightforward coplanar electrode layout and no particle pre-focusing mechanism is required. An original online algorithm analyzes the impedance signals of each incoming particle and regulates in real-time the dielectrophoretic voltages according to a desired control logic. As proof-of-concept, three operation modes are demonstrated on a mixture of 8, 10, and 12 µm diameter beads: (i) particle position swapping across channel axis, irrespective of particle size, (ii) size-based particle separation, irrespective of particle position, and (iii) sorting of a selected sequence of particles. As a perspective, the versatility of impedance cytometry and dielectrophoresis and the possibility to configure alternative control logics hold promises for advanced particle and cell manipulation.

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实时阻抗激活介电致动，实现单个流动粒子的可重构操纵

这项研究提出了一种用于选择性单粒子操作的创新型全电平台。该平台结合了用于无标记颗粒表征的微流体阻抗细胞仪和用于非接触式多向颗粒分离的介电泳技术。微流控芯片采用直接的共面电极布局，无需粒子预聚焦机制。一种独创的在线算法可分析每个进入粒子的阻抗信号，并根据所需的控制逻辑实时调节介电泳电压。作为概念验证，在直径分别为 8、10 和 12 微米的微珠混合物上演示了三种操作模式：(i) 颗粒位置跨通道轴交换，与颗粒大小无关；(ii) 基于大小的颗粒分离，与颗粒位置无关；(iii) 选定颗粒序列的分类。从这个角度来看，阻抗细胞仪和介质电泳的多功能性以及配置其他控制逻辑的可能性为先进的粒子和细胞操作带来了希望。

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来源期刊

Lab on a Chip 工程技术-化学综合

CiteScore

11.10

自引率

8.20%

发文量

434

审稿时长

2.6 months

期刊介绍： Lab on a Chip is the premiere journal that publishes cutting-edge research in the field of miniaturization. By their very nature, microfluidic/nanofluidic/miniaturized systems are at the intersection of disciplines, spanning fundamental research to high-end application, which is reflected by the broad readership of the journal. Lab on a Chip publishes two types of papers on original research: full-length research papers and communications. Papers should demonstrate innovations, which can come from technical advancements or applications addressing pressing needs in globally important areas. The journal also publishes Comments, Reviews, and Perspectives.