Effect of (R)-(−)-Linalool on endothelial damage: Sex differences

IF 2.3 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Biochemistry and Biophysics Reports Pub Date : 2024-10-15 DOI:10.1016/j.bbrep.2024.101846
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Abstract

Oxidative stress and inflammation are responsible for endothelial damage displaying many sex differences. Lipopolysaccharide (LPS) is a pathogenic stimulus that can trigger inflammation, contributing to endothelial dysfunction. Given the scientific evidence on the effectiveness of herbal extracts in managing endothelial dysfunction, we considered the (R)-(−)-Linalool (LIN), an aromatic monoterpene alcohol, as a bioactive phytochemical compound that could prevent and improve endothelial injury. In this study, we evaluated the effect of the LIN on LPS-induced damage in female and male human umbilical vein endothelial cells (FHUVECs and MHUVECs), measuring cell viability, cytokines release (IL-6 and TNF-α), malondialdehyde (MDA), and nitrites.
LPS significantly reduced viability both in MHUVECs and FHUVECs. Moreover, LPS increased the IL-6, TNF-α, and MDA level only in FHUVECs if compared to basal value; despite that, LPS reduced nitrites only in MHUVECs. LIN alone did not affect the parameters measured except for an increase in nitrites in FHUVECs. Nevertheless, LIN reduced damage and restored endothelium viability reduced by LPS without a clear sex difference. Under LPS, LIN inhibited IL-6 release and reduced MDA levels only in FHUVECs.
The present data confirm the existence of sex differences in the behavior of HUVECs under LPS conditions. The administration of LIN seems to have a more evident effect on FHUVECs after damage induced by LPS. These LIN effects are important to conduct further well-designed studies on the sex-specific use of this compound on vascular endothelial injury.

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(R)-(-)-芳樟醇对内皮损伤的影响:性别差异
氧化应激和炎症是造成内皮损伤的原因,并显示出许多性别差异。脂多糖(LPS)是一种致病性刺激物,可引发炎症,导致内皮功能障碍。鉴于有科学证据表明草药提取物可有效控制内皮功能障碍,我们认为芳香单萜醇 (R)-(-)- 芳樟醇 (LIN) 是一种生物活性植物化学物质,可预防和改善内皮损伤。在这项研究中,我们评估了 LIN 对 LPS 诱导的雌性和雄性人脐静脉内皮细胞(FHUVECs 和 MHUVECs)损伤的影响,测量了细胞活力、细胞因子释放量(IL-6 和 TNF-α)、丙二醛(MDA)和亚硝酸盐。此外,与基础值相比,LPS仅增加了FHUVECs的IL-6、TNF-α和MDA水平;尽管如此,LPS仅降低了MHUVECs的亚硝酸盐水平。除了亚硝酸盐在 FHUVECs 中增加外,LIN 本身对测量的参数没有影响。尽管如此,LIN 减少了 LPS 对内皮细胞的损伤,恢复了内皮细胞的活力,而且没有明显的性别差异。在 LPS 条件下,LIN 仅在 FHUVECs 中抑制 IL-6 的释放并降低 MDA 水平。在 LPS 诱导的损伤后,给予 LIN 似乎对 FHUVECs 有更明显的作用。LIN 的这些作用对于进一步精心设计该化合物对血管内皮损伤的性别特异性研究非常重要。
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来源期刊
Biochemistry and Biophysics Reports
Biochemistry and Biophysics Reports Biochemistry, Genetics and Molecular Biology-Biophysics
CiteScore
4.60
自引率
0.00%
发文量
191
审稿时长
59 days
期刊介绍: Open access, online only, peer-reviewed international journal in the Life Sciences, established in 2014 Biochemistry and Biophysics Reports (BB Reports) publishes original research in all aspects of Biochemistry, Biophysics and related areas like Molecular and Cell Biology. BB Reports welcomes solid though more preliminary, descriptive and small scale results if they have the potential to stimulate and/or contribute to future research, leading to new insights or hypothesis. Primary criteria for acceptance is that the work is original, scientifically and technically sound and provides valuable knowledge to life sciences research. We strongly believe all results deserve to be published and documented for the advancement of science. BB Reports specifically appreciates receiving reports on: Negative results, Replication studies, Reanalysis of previous datasets.
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