Comparison of experimental and simulated separation performance in capillary tube-in-manifold devices

IF 3.8 2区 化学 Q1 BIOCHEMICAL RESEARCH METHODS Journal of Chromatography A Pub Date : 2024-10-09 DOI:10.1016/j.chroma.2024.465428
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Abstract

A metal tube-in-manifold packed bed capillary column device, designed to overcome common limitations associated with capillary LC separations, is described. Experimental results of initial packing tests with sub-3 μm core-shell particles demonstrated efficiencies greater than 47,000 plates/m for a separation performed using the column device. Computational fluid dynamics (CFD) modeling of the multicomponent separation used for this work was validated against experimental LC results and the optimized model was able to effectively predict component peak retention times. However, the accuracy of predicted efficiencies requires further refinement. The tube-in-manifold design demonstrates that packed capillary columns with cylindrical cross-sectional channel geometry and ultrahigh pressure, low dead volume fluidic connections are achievable.
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毛细管内成型装置的实验和模拟分离性能比较
本文介绍了一种金属管-歧管填料床毛细管柱装置,该装置旨在克服与毛细管液相色谱分离相关的常见限制。使用 3 μm 以下核壳颗粒进行的初步填料测试的实验结果表明,使用该色谱柱装置进行分离的效率超过 47,000 板/米。这项工作中使用的多组分分离计算流体动力学(CFD)模型与 LC 实验结果进行了验证,优化后的模型能够有效预测组分的峰值保留时间。然而,预测效率的准确性还需要进一步改进。管中管设计表明,具有圆柱形横截面通道几何形状和超高压、低死体积流体连接的填料毛细管柱是可以实现的。
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来源期刊
Journal of Chromatography A
Journal of Chromatography A 化学-分析化学
CiteScore
7.90
自引率
14.60%
发文量
742
审稿时长
45 days
期刊介绍: The Journal of Chromatography A provides a forum for the publication of original research and critical reviews on all aspects of fundamental and applied separation science. The scope of the journal includes chromatography and related techniques, electromigration techniques (e.g. electrophoresis, electrochromatography), hyphenated and other multi-dimensional techniques, sample preparation, and detection methods such as mass spectrometry. Contributions consist mainly of research papers dealing with the theory of separation methods, instrumental developments and analytical and preparative applications of general interest.
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