Insecticide exposure can increase burrow network production and alter burrow network structure in soil dwelling insects (Agriotes spp.)

Abstract

Insecticide treated seeds are commonly used to reduce yield losses from burrowing insect damage such as wireworms. Using temporal X-ray Computed Tomography (CT) of soil-filled bioassays, we aimed to quantify changes in burrow network production and structure as a measure of wireworm behavioural change in response to three types of insecticide treated maize seed; compound X (R&D product in field trial stage of development); tefluthrin and thiamethoxam. A biopesticide alternative treatment (neem), untreated maize seed and bare soil were also investigated. Insect health outcomes were also monitored to provide toxicity/mortality data. Wireworms exposed to compound X produced greater burrow networks than untreated maize and neem treatments, similar to that in volume of those produced in bare soil. Compound X exposure also elicited the production of more complex burrow structures, a function of the number of vertices, edges and faces of a shape (V-E+F) related to the number of interconnected branches, compared to any other treatments. Compound X, tefluthrin and thiamethoxam induced mortality at greater rates than neem or untreated, suggesting all three could have potential to manage wireworm populations and reduce yield loss, but only compound X modified burrowing behaviour. With soil biopores playing an important role in soil productivity and carbon sequestration, the wider implications of this increase in burrowing activity for food security and climate change warrants further exploration.