Using proteomic approaches to predict particulate matter stress response of ornamental plant

IF 2.4 4区生物学 Q2 PLANT SCIENCES Acta Physiologiae Plantarum Pub Date : 2024-10-14 DOI:10.1007/s11738-024-03714-z

Arnon Setsungnern, Chairat Treesubsuntorn, Waleeporn Pongkua, Wararat Sriprapat, Sucheewin Krobthong, Yodying Yingchutrakul, Paitip Thiravetyan

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Abstract

Previous studies reported that plants’ exposure to particulate matter (PM) may decrease the PM phytoremediation efficiency. This study aims to investigate the effect of PM on plants at the proteomic level. Here, five plant species were fumigated with cigarette PM (the initial PM_2.5 of 470–500 µg/m³) in a 15.6 L testing chamber for 6 days. PM removal efficiency and absorption were evaluated for protein pattern between plants exposed and non-exposure to PM for 24 h. The results showed that Z. zamiifolia and S. trifasciata can effectively remove indoor PM (35–40 μg/m³). Also, several plant proteins (heat shock, aconitate hydratase 3, chaperone protein, NADP-dependent malic enzyme, probably aquaporin TIP2-2, serine hydroxymethyltransferase 2, ATP-dependent zinc metalloprotease FTSH 8, V-type proton ATPase subunit B2, malate dehydrogenase, peptidyl-prolyl cis–trans isomerase, CYP38, peroxiredoxin-2E, and histone) reported significantly upregulated and downregulated about 100 times after PM exposure than control. Proteins related to water accumulation and H₂O₂ scavenging significantly changed (p value < 0.05) after PM exposure. PM concentration was found low (< 0.5 mg/m²) on the leaf surface after 150 h exposure. Herein, water accumulation and H₂O₂ scavenging protein might play an important role in plant PM stress.

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利用蛋白质组学方法预测观赏植物的颗粒物质应激反应

以往的研究表明，植物暴露于颗粒物可能会降低颗粒物的植物修复效率。本研究旨在从蛋白质组水平研究可吸入颗粒物对植物的影响。在这里，五种植物在一个 15.6 升的试验箱内用香烟可吸入颗粒物（初始 PM2.5 为 470-500 µg/m3）熏蒸了 6 天。结果表明，Z. zamiifolia 和 S. trifasciata 能有效去除室内可吸入颗粒物（35-40 μg/m3）。此外，几种植物蛋白（热休克蛋白、乌头水解酶 3、伴侣蛋白、NADP 依赖性苹果酸酶、可能的水蒸气蛋白 TIP2-2、丝氨酸羟甲基转移酶 2、ATP 依赖性锌金属蛋白酶 FTSH 8、据报道，与对照组相比，暴露于 PM 后，V 型质子 ATP 酶亚基 B2、苹果酸脱氢酶、肽基-脯氨酰顺反异构酶、CYP38、过氧化物酶-2E 和组蛋白的上调和下调幅度分别为 100 倍左右。与蓄水和清除 H2O2 有关的蛋白质在接触 PM 后发生了明显变化（P 值为 0.05）。暴露 150 小时后，叶片表面的 PM 浓度很低（0.5 mg/m2）。由此可见，水分积累和 H2O2 清除蛋白可能在植物 PM 胁迫中发挥了重要作用。

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来源期刊

Acta Physiologiae Plantarum 生物-植物科学

CiteScore

5.10

自引率

3.80%

发文量

125

审稿时长

3.1 months

期刊介绍： Acta Physiologiae Plantarum is an international journal established in 1978 that publishes peer-reviewed articles on all aspects of plant physiology. The coverage ranges across this research field at various levels of biological organization, from relevant aspects in molecular and cell biology to biochemistry. The coverage is global in scope, offering articles of interest from experts around the world. The range of topics includes measuring effects of environmental pollution on crop species; analysis of genomic organization; effects of drought and climatic conditions on plants; studies of photosynthesis in ornamental plants, and more.