Colistin, doxycycline and Labetalol-meropenem combination are the most active against XDR-Carbapenem-resistant Acinetobacter baumannii: Role of a novel transferrable plasmid conferring carbapenem resistance

IF 2.1 4区医学 Q3 INFECTIOUS DISEASES Diagnostic microbiology and infectious disease Pub Date : 2024-10-12 DOI:10.1016/j.diagmicrobio.2024.116558

Heba Mohammed Refat M. Selim , Fatma Alzahraa M. Gomaa , Mohammad Y. Alshahrani , Noha A. Kamel , Khaled M. Aboshanab , Khaled M. Elsayed

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Abstract

This study aimed to evaluate the antimicrobial susceptibility and combination of a beta-blocker, labetalol (LAB) and meropenem (MEM) on Carbapenem-resistant (CR) A. baumannii clinical isolates. A total of 43 CR- A. baumannii were isolated of which 37 (86.6 %) and 28 (65 %) exhibited MDR and XDR phenotypes, respectively. Colistin and doxycycline still retain their activities in 93.1 % and 72.1 % of the isolates, respectively. Combining MEM with LAB at 0.25 mg /mL, decreased MIC values in 91.4 % (32/35) however, at 0.5 mg /mL, it decreased MIC value and restored susceptibility to MEM in 100 % and 91.4 % of the tested isolates, respectively. A novel transferable plasmid pAcbGIM3 harboring aph-3′, bla_oxa-58, bla_GIM3 and bla_CTX-M3 and eight mobile genetic elements were successfully isolated from a pan-drug resistant (PDR) isolate. In conclusion, LAB-MEM is a promising combination and should be clinically examined. This is the first report of a transmissible plasmid harboring bla_GIM3 gene in Egypt.

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可乐定、强力霉素和拉贝洛尔-美罗培南组合对耐XDR-碳青霉烯类鲍曼不动杆菌最有效：赋予碳青霉烯耐药性的新型可转移质粒的作用

本研究旨在评估β-受体阻滞剂拉贝洛尔（LAB）和美罗培南（MEM）对耐碳青霉烯类（CR）鲍曼氏菌临床分离株的抗菌药敏感性和联合用药情况。共分离出 43 株对卡巴培南耐药的鲍曼尼氏菌，其中 37 株（86.6%）和 28 株（65%）分别表现出 MDR 和 XDR 表型。可乐定和强力霉素仍分别在 93.1% 和 72.1% 的分离物中保持活性。在 0.25 毫克/毫升的浓度下，将 MEM 与 LAB 结合使用可降低 91.4% （32/35）的 MIC 值，但在 0.5 毫克/毫升的浓度下，MEM 可降低 100% 的 MIC 值，并使 91.4% 的受试分离物恢复对 MEM 的敏感性。从一个具有泛耐药性（PDR）的分离株中成功分离出了一个新型可转移质粒 pAcbGIM3，该质粒含有 aph-3′、blaoxa-58、blaGIM3 和 blaCTX-M3 以及八个移动遗传元件。总之，LAB-MEM 是一种很有前景的组合，应在临床上加以研究。这是埃及首次报道携带 blaGIM3 基因的可传播质粒。

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来源期刊

Diagnostic microbiology and infectious disease 医学-传染病学

CiteScore

5.30

自引率

3.40%

发文量

149

审稿时长

56 days

期刊介绍： Diagnostic Microbiology and Infectious Disease keeps you informed of the latest developments in clinical microbiology and the diagnosis and treatment of infectious diseases. Packed with rigorously peer-reviewed articles and studies in bacteriology, immunology, immunoserology, infectious diseases, mycology, parasitology, and virology, the journal examines new procedures, unusual cases, controversial issues, and important new literature. Diagnostic Microbiology and Infectious Disease distinguished independent editorial board, consisting of experts from many medical specialties, ensures you extensive and authoritative coverage.