Rosalía López-Corrales, Sami J. Michereff, Kamila Câmara Correia, Raymundo S. García-Estrada, Edgar H. Nieto-López, Guadalupe A. Mora-Romero, Juan M. Tovar-Pedraza
{"title":"Molecular Identification and Fungicide Sensitivity of Ceratobasidium sp. (AG-A) Isolates Causing Stem Canker on Potato in Mexico","authors":"Rosalía López-Corrales, Sami J. Michereff, Kamila Câmara Correia, Raymundo S. García-Estrada, Edgar H. Nieto-López, Guadalupe A. Mora-Romero, Juan M. Tovar-Pedraza","doi":"10.1111/jph.13412","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>In 2022, potato plants exhibiting symptoms of stem canker were observed in commercial fields in northern Sinaloa, Mexico. Morphological characterisation of two isolates allowed the identification of <i>Ceratobasidium</i> sp., whereas molecular analysis using sequences of the ITS region and the <i>rpb2</i> gene distinguished the isolates within the Anastomosis Group A (AG-A). Both isolates induced symptoms of stem canker on inoculated potato plants and exhibited median EC<sub>50</sub> values of 19.46, 5.20, 62.71 and 0.33 μg mL<sup>−1</sup> for the fungicides thiophanate methyl, pyraclostrobin, prochloraz and penflufen, respectively. However, the two isolates differed by > 65-fold in their EC<sub>50</sub> value relative to pyraclostrobin.</p>\n </div>","PeriodicalId":16843,"journal":{"name":"Journal of Phytopathology","volume":null,"pages":null},"PeriodicalIF":1.1000,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Phytopathology","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/jph.13412","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
In 2022, potato plants exhibiting symptoms of stem canker were observed in commercial fields in northern Sinaloa, Mexico. Morphological characterisation of two isolates allowed the identification of Ceratobasidium sp., whereas molecular analysis using sequences of the ITS region and the rpb2 gene distinguished the isolates within the Anastomosis Group A (AG-A). Both isolates induced symptoms of stem canker on inoculated potato plants and exhibited median EC50 values of 19.46, 5.20, 62.71 and 0.33 μg mL−1 for the fungicides thiophanate methyl, pyraclostrobin, prochloraz and penflufen, respectively. However, the two isolates differed by > 65-fold in their EC50 value relative to pyraclostrobin.
期刊介绍:
Journal of Phytopathology publishes original and review articles on all scientific aspects of applied phytopathology in agricultural and horticultural crops. Preference is given to contributions improving our understanding of the biotic and abiotic determinants of plant diseases, including epidemics and damage potential, as a basis for innovative disease management, modelling and forecasting. This includes practical aspects and the development of methods for disease diagnosis as well as infection bioassays.
Studies at the population, organism, physiological, biochemical and molecular genetic level are welcome. The journal scope comprises the pathology and epidemiology of plant diseases caused by microbial pathogens, viruses and nematodes.
Accepted papers should advance our conceptual knowledge of plant diseases, rather than presenting descriptive or screening data unrelated to phytopathological mechanisms or functions. Results from unrepeated experimental conditions or data with no or inappropriate statistical processing will not be considered. Authors are encouraged to look at past issues to ensure adherence to the standards of the journal.