Combination therapy of placenta-derived mesenchymal stem cells and artificial dermal scaffold promotes full-thickness skin defects vascularization in rat animal model

IF 2.5 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL Advances in medical sciences Pub Date : 2024-10-17 DOI:10.1016/j.advms.2024.10.002

Kun Zhang , Dongjie Xiao , Fang Li , Guodong Song , Guobao Huang , Yunshan Wang , Hua Liu

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Abstract

Purpose

Recently, placenta-derived mesenchymal stem cells (PMSCs) have garnered considerable attention in tissue repair and regeneration. The present study was conducted to evaluate the effect of PMSCs on artificial dermal scaffold (ADS) angiogenesis and their combination therapy on wound closure.

Material and methods

Herein, the growth and survival of PMSCs in ADS were explored. CCK8, scratch wound, and tubule formation assays were employed to investigate the effects of ADS conditioned medium (CM) and ADS-PMSCs CM on human umbilical vein endothelial cells (HUVECs). The effect of ADS-PMSCs on full-thickness skin defects healing was evaluated based on a rat model. Wound healing progresses was meticulously investigated through hematoxylin and eosin (HE), Masson's trichrome, and immunohistochemical staining analyses.

Results

In vitro cell culture results demonstrated the proliferation of PMSCs in ADS. The ADS-PMSCs CM notably stimulated the proliferation, migration, and tube formation of HUVECs compared to the ADS CM group. In the rat full-thickness skin defect model, the ADS-PMSCs treatment significantly accelerated the vascularization area of ADS after 2 weeks. Besides, HE and Masson's trichrome staining results indicated that ADS-PMSCs treatment significantly enhanced fibroblast proliferation and collagen fiber 2 weeks after surgical procedure. Compared to the ADS group, collagen fiber arrangement was thicker in the ADS-PMSCs group. Immunohistochemical staining reinforced this finding, illustrating a substantial increase in CD31 expression within the ADS-PMSCs group.

Conclusions

The results suggest that the combination of ADS with PMSCs accelerates ADS vascularization by fostering granulation tissue development and boosting the formation of new blood vessels.

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胎盘间充质干细胞与人工真皮支架的联合疗法促进了大鼠动物模型全厚皮肤缺损血管的形成。

目的：最近，胎盘间充质干细胞（PMSCs）在组织修复和再生方面受到广泛关注。本研究旨在评估胎盘间充质干细胞对人工真皮支架（ADS）血管生成的影响及其对伤口闭合的联合治疗作用。采用 CCK8、划痕伤口和小管形成试验研究 ADS 条件培养基（CM）和 ADS-PMSCs CM 对人脐静脉内皮细胞（HUVECs）的影响。以大鼠模型为基础，评估了 ADS-PMSCs 对全厚皮肤缺损愈合的影响。通过苏木精和伊红（HE）、马森三色染色和免疫组化染色分析，对伤口愈合进展进行了细致的研究：体外细胞培养结果表明，PMSCs 在 ADS 中增殖。与 ADS CM 组相比，ADS-PMSCs CM 组明显刺激了 HUVECs 的增殖、迁移和管形成。在大鼠全厚皮肤缺损模型中，ADS-PMSCs 治疗 2 周后明显加快了 ADS 的血管化面积。此外，HE 和 Masson's trichrome 染色结果表明，ADS-PMSCs 治疗能明显促进成纤维细胞的增殖和胶原纤维的形成。与 ADS 组相比，ADS-PMSCs 组的胶原纤维排列更粗。免疫组化染色证实了这一发现，表明 ADS-PMSCs 组 CD31 表达量大幅增加：结论：研究结果表明，ADS 与 PMSCs 的结合可促进肉芽组织的发育和新血管的形成，从而加速 ADS 的血管化。

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来源期刊

Advances in medical sciences 医学-医学：研究与实验

CiteScore

5.00

自引率

0.00%

发文量

审稿时长

25 days

期刊介绍： Advances in Medical Sciences is an international, peer-reviewed journal that welcomes original research articles and reviews on current advances in life sciences, preclinical and clinical medicine, and related disciplines. The Journal’s primary aim is to make every effort to contribute to progress in medical sciences. The strive is to bridge laboratory and clinical settings with cutting edge research findings and new developments. Advances in Medical Sciences publishes articles which bring novel insights into diagnostic and molecular imaging, offering essential prior knowledge for diagnosis and treatment indispensable in all areas of medical sciences. It also publishes articles on pathological sciences giving foundation knowledge on the overall study of human diseases. Through its publications Advances in Medical Sciences also stresses the importance of pharmaceutical sciences as a rapidly and ever expanding area of research on drug design, development, action and evaluation contributing significantly to a variety of scientific disciplines. The journal welcomes submissions from the following disciplines: General and internal medicine, Cancer research, Genetics, Endocrinology, Gastroenterology, Cardiology and Cardiovascular Medicine, Immunology and Allergy, Pathology and Forensic Medicine, Cell and molecular Biology, Haematology, Biochemistry, Clinical and Experimental Pathology.