Development of pseudotyped VSV-SARS-CoV-2 spike variants for the assessment of neutralizing antibodies.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2024-10-16 DOI:10.1080/17576180.2024.2411920

Atike Nur Cimen, Gizem Celebi Torabfam, Yesim Tuyji Tok, Ebru Yucebag, Nese Arslan, Devrim Saribal, Gulen Esken, Ozlem Dogan, Mert Ahmet Kuskucu, Bilgul Mete, Gokhan Aygun, Fehmi Tabak, Fusun Can, Onder Ergonul, Kenan Midilli, Ozlem Kutlu, Sibel Cetinel

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Abstract

Aim: Serological studies with pseudotyped viruses offer a safer alternative to live SARS-CoV-2 in evaluating neutralizing antibodies, enabling research in standard labs.Methods: The SARS-CoV-2 Spike pseudotyped vesicular stomatitis virus (VSV) pseudoviruses were generated using Spike of Wuhan strain and two variants (B.1.1.7, B.1.351) and utilized to evaluate the serum neutralizing activity of human plasma samples of vaccinated (n = 13) and healthy people (n = 2) compared with a plaque assay with authentic virus.Results: Neutralizing titer of convalescent plasma resulted with a good correlation (R² = 0.7).Conclusion: We evaluated a safe and reliable pseudotyped virus system that effectively mimics authentic virus and correlates well with traditional assays. The developed system allows easier testing of variants and has the potential to improve vaccine development.

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开发用于评估中和抗体的伪型 VSV-SARS-CoV-2 穗状变体。

目的：在评估中和抗体时，使用伪病毒进行血清学研究比使用活的 SARS-CoV-2 更安全，可在标准实验室中进行研究：方法：利用武汉株 Spike 和两个变异株（B.1.1.7 和 B.1.351）生成了 SARS-CoV-2 Spike 伪型水泡性口炎病毒（VSV）伪病毒，并与真病毒斑块试验相比，评估了接种疫苗者（13 人）和健康者（2 人）血浆样本的血清中和活性：结果：康复血浆的中和滴度具有良好的相关性（R2 = 0.7）：我们评估了一种安全可靠的伪型病毒系统，它能有效模拟真病毒，并与传统检测方法有很好的相关性。所开发的系统可以更方便地测试变异株，并有可能改进疫苗开发。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.

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