Development of pseudotyped VSV-SARS-CoV-2 spike variants for the assessment of neutralizing antibodies.

IF 1.8 4区医学 Q3 BIOCHEMICAL RESEARCH METHODS Bioanalysis Pub Date : 2024-01-01 Epub Date: 2024-10-16 DOI:10.1080/17576180.2024.2411920

Atike Nur Cimen, Gizem Celebi Torabfam, Yesim Tuyji Tok, Ebru Yucebag, Nese Arslan, Devrim Saribal, Gulen Esken, Ozlem Dogan, Mert Ahmet Kuskucu, Bilgul Mete, Gokhan Aygun, Fehmi Tabak, Fusun Can, Onder Ergonul, Kenan Midilli, Ozlem Kutlu, Sibel Cetinel

{"title":"Development of pseudotyped VSV-SARS-CoV-2 spike variants for the assessment of neutralizing antibodies.","authors":"Atike Nur Cimen, Gizem Celebi Torabfam, Yesim Tuyji Tok, Ebru Yucebag, Nese Arslan, Devrim Saribal, Gulen Esken, Ozlem Dogan, Mert Ahmet Kuskucu, Bilgul Mete, Gokhan Aygun, Fehmi Tabak, Fusun Can, Onder Ergonul, Kenan Midilli, Ozlem Kutlu, Sibel Cetinel","doi":"10.1080/17576180.2024.2411920","DOIUrl":null,"url":null,"abstract":"Aim: Serological studies with pseudotyped viruses offer a safer alternative to live SARS-CoV-2 in evaluating neutralizing antibodies, enabling research in standard labs.Methods: The SARS-CoV-2 Spike pseudotyped vesicular stomatitis virus (VSV) pseudoviruses were generated using Spike of Wuhan strain and two variants (B.1.1.7, B.1.351) and utilized to evaluate the serum neutralizing activity of human plasma samples of vaccinated (n = 13) and healthy people (n = 2) compared with a plaque assay with authentic virus.Results: Neutralizing titer of convalescent plasma resulted with a good correlation (R2 = 0.7).Conclusion: We evaluated a safe and reliable pseudotyped virus system that effectively mimics authentic virus and correlates well with traditional assays. The developed system allows easier testing of variants and has the potential to improve vaccine development.","PeriodicalId":8797,"journal":{"name":"Bioanalysis","volume":" ","pages":"1167-1177"},"PeriodicalIF":1.8000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11583609/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioanalysis","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/17576180.2024.2411920","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/10/16 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}

引用次数: 0

Abstract

Aim: Serological studies with pseudotyped viruses offer a safer alternative to live SARS-CoV-2 in evaluating neutralizing antibodies, enabling research in standard labs.Methods: The SARS-CoV-2 Spike pseudotyped vesicular stomatitis virus (VSV) pseudoviruses were generated using Spike of Wuhan strain and two variants (B.1.1.7, B.1.351) and utilized to evaluate the serum neutralizing activity of human plasma samples of vaccinated (n = 13) and healthy people (n = 2) compared with a plaque assay with authentic virus.Results: Neutralizing titer of convalescent plasma resulted with a good correlation (R² = 0.7).Conclusion: We evaluated a safe and reliable pseudotyped virus system that effectively mimics authentic virus and correlates well with traditional assays. The developed system allows easier testing of variants and has the potential to improve vaccine development.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

开发用于评估中和抗体的伪型 VSV-SARS-CoV-2 穗状变体。

目的：在评估中和抗体时，使用伪病毒进行血清学研究比使用活的 SARS-CoV-2 更安全，可在标准实验室中进行研究：方法：利用武汉株 Spike 和两个变异株（B.1.1.7 和 B.1.351）生成了 SARS-CoV-2 Spike 伪型水泡性口炎病毒（VSV）伪病毒，并与真病毒斑块试验相比，评估了接种疫苗者（13 人）和健康者（2 人）血浆样本的血清中和活性：结果：康复血浆的中和滴度具有良好的相关性（R2 = 0.7）：我们评估了一种安全可靠的伪型病毒系统，它能有效模拟真病毒，并与传统检测方法有很好的相关性。所开发的系统可以更方便地测试变异株，并有可能改进疫苗开发。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Bioanalysis BIOCHEMICAL RESEARCH METHODS-CHEMISTRY, ANALYTICAL

CiteScore

3.30

自引率

16.70%

发文量

审稿时长

2 months

期刊介绍： Reliable data obtained from selective, sensitive and reproducible analysis of xenobiotics and biotics in biological samples is a fundamental and crucial part of every successful drug development program. The same principles can also apply to many other areas of research such as forensic science, toxicology and sports doping testing. The bioanalytical field incorporates sophisticated techniques linking sample preparation and advanced separations with MS and NMR detection systems, automation and robotics. Standards set by regulatory bodies regarding method development and validation increasingly define the boundaries between speed and quality. Bioanalysis is a progressive discipline for which the future holds many exciting opportunities to further reduce sample volumes, analysis cost and environmental impact, as well as to improve sensitivity, specificity, accuracy, efficiency, assay throughput, data quality, data handling and processing. The journal Bioanalysis focuses on the techniques and methods used for the detection or quantitative study of analytes in human or animal biological samples. Bioanalysis encourages the submission of articles describing forward-looking applications, including biosensors, microfluidics, miniaturized analytical devices, and new hyphenated and multi-dimensional techniques. Bioanalysis delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for the modern bioanalyst.