Exosomal therapy mitigates silver nanoparticles-induced neurotoxicity in rats.

IF 2 4区医学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biomarkers Pub Date : 2024-11-01 Epub Date: 2024-10-17 DOI:10.1080/1354750X.2024.2415072

Hanan Safwat Salah Elden Hassan, Walaa A Moselhy, Marwa A Ibrahim, Ayman H Zaki, Fatma Khalil, Eman I Hassanen, Doaa R I Abdel-Gawad

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Abstract

Introduction: Our investigation aims to appraise the neuroprotective impact of Bone Marrow-Mesenchymal Stem Cells (BM-MSCs) derived exosomes against Ag NPs-inducing neurotoxicity in rats.

Materials and methods: Twenty-four albino rats were divided into 3 groups. Group I (control negative), Group II (intraperitoneally injected with Ag NPs for 28 days, whereas Group III (intraperitoneally injected with Ag NP and BM-MSCs derived exosomes.

Results: There was a marked elevation of Malondialdehyde (MDA) along with a reduction of brain antioxidants, Gamma-aminobutyric acid (GABA) and Monoamine Oxidase (MAO) in the Ag NPs receiving group. Ag NPs upregulated c-Jun N-terminal Kinases (JNK) genes and c-Myc and downregulated the tissue inhibitors of metalloproteinases (TIMP-1) and Histone deacetylase 1 (HDAC1) genes. Otherwise, the co-treatment of BM-MSCs derived exosomes with Ag NPs could markedly increase the rat's body weight, activity and learning while, decreasing anxiety, restoring all the toxicological parameters and improving the microscopic appearance of different brain areas.

Conclusion: BM-MSCs-derived exosomes downregulated both apoptotic and inflammatory mediators and upregulated the antiapoptotic genes. BM-MSCs-derived exosomes exhibit a great therapeutic effect against the neurotoxic effects of Ag NPs.

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外泌体疗法可减轻银纳米粒子诱导的大鼠神经毒性。

简介：我们的研究旨在评估骨髓间充质干细胞（BM-MSCs）衍生的外泌体对大鼠Ag NPs诱导神经毒性的神经保护作用：将 24 只白化大鼠分为 3 组。I组（阴性对照组）、II组（腹腔注射Ag NPs 28天）和III组（腹腔注射Ag NP和BM-MSCs衍生外泌体：结果：Ag NPs接受组的丙二醛（MDA）明显升高，脑抗氧化剂、γ-氨基丁酸（GABA）和单胺氧化酶（MAO）减少。Ag NPs上调了c-Jun N-terminal Kinases（JNK）基因和c-Myc基因，下调了组织金属蛋白酶抑制剂（TIMP-1）和组蛋白去乙酰化酶1（HDAC1）基因。此外，BM-间充质干细胞衍生的外泌体与Ag NPs联合处理可显著增加大鼠的体重、活动和学习能力，同时降低焦虑，恢复所有毒理学参数，改善不同脑区的显微外观：结论：源自骨髓间充质干细胞的外泌体可下调凋亡和炎症介质，并上调抗凋亡基因。结论：间充质干细胞衍生的外泌体可降低凋亡和炎症介质，并上调抗凋亡基因。

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来源期刊

Biomarkers 医学-毒理学

CiteScore

5.00

自引率

3.80%

发文量

140

审稿时长

3 months

期刊介绍： The journal Biomarkers brings together all aspects of the rapidly growing field of biomarker research, encompassing their various uses and applications in one essential source. Biomarkers provides a vital forum for the exchange of ideas and concepts in all areas of biomarker research. High quality papers in four main areas are accepted and manuscripts describing novel biomarkers and their subsequent validation are especially encouraged: • Biomarkers of disease • Biomarkers of exposure • Biomarkers of response • Biomarkers of susceptibility Manuscripts can describe biomarkers measured in humans or other animals in vivo or in vitro. Biomarkers will consider publishing negative data from studies of biomarkers of susceptibility in human populations.