Exosomes Derived from Bone Marrow Dendritic Cells Exhibit Protective and Therapeutic Potential Against Chemically Induced Chronic Pancreatitis in Rats.

IF 4.5 2区医学 Q2 CELL BIOLOGY Inflammation Pub Date : 2024-10-19 DOI:10.1007/s10753-024-02150-y

Shaimaa M Bashir, Sherine M Rizk, Mohammed M Nooh, Hebatullah S Helmy

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Abstract

Background: Chronic pancreatitis (CP) is a specific clinical disorder that develops from pancreatic fibrosis and immune cell dysregulation. It has been proposed that bone marrow dendritic cells (BMDCs) exosomes have significant effects on immune regulation. Thus, the current study acquainted the prophylactic and therapeutic effects of exosomes derived from BMDCs on a rat model of CP.

Materials and methods: BMDCs were prepared and identified, and then the exosomes were isolated by differential ultracentrifugation. Prophylactic and therapeutic effects of exosomes were investigated on L-arginine induced CP model.

Results: Administration of two tail vein injections of exosomes (200 μg/kg/dose suspended in 0.2 ml PBS) markedly improved the pancreatic function and histology compared to CP group. Moreover, exosomes prominently mitigated the increase in amylase, lipase, tumor necrosis factor-α (TNF-α), transforming growth factor-β (TGF-β) and elevated antioxidant enzymes; catalase, superoxide dismutase (SOD) and glutathione peroxidase (GPx).

Conclusion: BMDCs exosomes can be considered as a promising candidate, with a high efficacy and stability compared with its parent cell, for management of CP and similar inflammatory diseases.

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骨髓树突状细胞提取的外泌体对化学药物诱导的大鼠慢性胰腺炎具有保护和治疗潜力

背景：慢性胰腺炎（CP）是一种特殊的临床疾病，由胰腺纤维化和免疫细胞失调引起。有研究认为，骨髓树突状细胞（BMDCs）外泌体对免疫调节有重要影响。因此，本研究了解了骨髓树突状细胞外泌体对CP大鼠模型的预防和治疗作用：制备并鉴定 BMDCs，然后通过差速超速离心法分离外泌体。研究了外泌体对外精氨酸诱导的 CP 模型的预防和治疗作用：结果：与 CP 组相比，外泌体（200 μg/kg/dose 悬浮于 0.2 ml PBS 中）尾静脉注射两次可明显改善 CP 组的胰腺功能和组织学。此外，外泌体还显著缓解了淀粉酶、脂肪酶、肿瘤坏死因子-α（TNF-α）、转化生长因子-β（TGF-β）的增加，以及过氧化氢酶、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPx）等抗氧化酶的升高：结论：与母细胞相比，BMDCs 外泌体具有更高的疗效和稳定性，可被视为治疗 CP 和类似炎症性疾病的有效候选物质。

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来源期刊

Inflammation 医学-免疫学

CiteScore

9.70

自引率

0.00%

发文量

168

审稿时长

3.0 months

期刊介绍： Inflammation publishes the latest international advances in experimental and clinical research on the physiology, biochemistry, cell biology, and pharmacology of inflammation. Contributions include full-length scientific reports, short definitive articles, and papers from meetings and symposia proceedings. The journal''s coverage includes acute and chronic inflammation; mediators of inflammation; mechanisms of tissue injury and cytotoxicity; pharmacology of inflammation; and clinical studies of inflammation and its modification.