Thioclava arctica sp. nov. and Zhongshania arctica sp. nov., isolated from the surface sediment of the Arctic Ocean and reclassification of Marortus luteolus Yu et al. 2019 as a later heterotypic synonym of Zhongshania marina On et al. 2019.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2024-10-01 DOI:10.1099/ijsem.0.006548
Panyi Pan, Jianning Wang, Xiupian Liu, Qiliang Lai, Guangyu Li, Yaping Du, Jinzhu Zhang, Ziyuan He, Zongze Shao, Chunming Dong
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引用次数: 0

Abstract

Two Gram-stain-negative bacterial strains, 15-R06ZXC-3T and R06B22T, were isolated from the surface sediment of the Arctic Ocean. Phylogenetic analyses based on the 16S rRNA gene and genome sequences indicated that strain 15-R06ZXC-3T belongs to the genus Thioclava, while strain R06B22T belongs to the genus Zhongshania. Strain 15-R06ZXC-3T showed the closest relationship to Thioclava indica DT23-4T. The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strain 15-R06ZXC-3T and all of the type strains of the genus Thioclava ranged from 20.8 to 30.4% and 79.1 to 85.7%, respectively. Strain R06B22T was most closely related to Zhongshania marina DSW25-10T. The dDDH and ANI values between strain R06B22T and all of the type strains of the genus Zhongshania ranged from 18.6 to 20.4% and 77.5 to 79.4%, respectively. These dDDH and ANI values were all below the standard cutoff criteria for the delineation of bacterial species, indicating that the two strains may represent novel species within their respective genera. Furthermore, their phenotypic and chemotaxonomic characteristics also differentiated them from closely related species. Based on the polyphasic analyses, strains 15-R06ZXC-3T and R06B22T separately represent novel species of the genera Thioclava and Zhongshania, for which the names Thioclava arctica sp. nov. (type strain 15-R06ZXC-3T = MCCC 1A07434T= KCTC 8342T) and Zhongshania arctica sp. nov. (type strain R06B22T = MCCC 1A08273T= KCTC 8343T) are proposed. Additionally, phylogenomic analyses showed that the strain Marortus luteolus ZX-21T was clustered with the strain Z. marina DSW25-10T and all other type strains of the genus Zhongshania. Furthermore, the ANI and dDDH values between strains ZX-21T and DSW25-10T were 97.6% and 78.8±2.5%, respectively, strongly indicating that they represented a single species. Therefore, it is proposed that M. luteolus Yu et al. 2019 be recognized as a later heterotypic synonym of Z. marina On et al. 2019.

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从北冰洋表层沉积物中分离的 Thioclava arctica sp.
从北冰洋表层沉积物中分离出两株革兰氏染色阴性细菌--15-R06ZXC-3T和R06B22T。基于 16S rRNA 基因和基因组序列的系统进化分析表明,菌株 15-R06ZXC-3T 属于 Thioclava 属,而菌株 R06B22T 属于 Zhongshania 属。菌株 15-R06ZXC-3T 与 Thioclava indica DT23-4T 的关系最为密切。菌株 15-R06ZXC-3T 与 Thioclava 属所有模式菌株之间的数字 DNA-DNA 杂交(dDDH)和平均核苷酸同一性(ANI)值分别为 20.8%至 30.4%和 79.1%至 85.7%。菌株 R06B22T 与 Zhongshania marina DSW25-10T 的亲缘关系最为密切。菌株 R06B22T 与中沙藻属所有类型菌株之间的 dDDH 值和 ANI 值分别为 18.6%至 20.4%和 77.5%至 79.4%。这些 dDDH 值和 ANI 值均低于划分细菌物种的标准临界值,表明这两株菌株可能是各自属中的新物种。此外,它们的表型和化学分类学特征也将它们与近缘物种区分开来。根据多相分析,15-R06ZXC-3T 和 R06B22T 菌株分别代表 Thioclava 属和 Zhongshania 属的新种,并将其命名为 Thioclava arctica sp.nov.(模式菌株 15-R06ZXC-3T = MCCC 1A07434T= KCTC 8342T)和 Zhongshania arctica sp.nov.(模式菌株 R06B22T = MCCC 1A08273T= KCTC 8343T)。此外,系统发生组分析表明,Marortus luteolus ZX-21T 菌株与 Z. marina DSW25-10T 菌株以及 Zhongshania 属的所有其他模式菌株聚类。此外,菌株 ZX-21T 与 DSW25-10T 之间的 ANI 值和 dDDH 值分别为 97.6% 和 78.8±2.5%,有力地表明它们代表一个物种。因此,建议将 M. luteolus Yu 等(2019)认定为 Z. marina On 等(2019)的异型异名。
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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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