Marine Bacillus pumilus substances exhibit antimicrobial effect on multidrug-resistant Staphylococcus aureus.

IF 3.2 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Journal of Applied Microbiology Pub Date : 2024-10-03 DOI:10.1093/jambio/lxae260

Jéssyca Freitas-Silva, Roberto Carlos Campos Martins, Carla Monteiro Leal, Simony Carvalho Mendonça, Jefferson Bomfim Silva Cypriano, Walter Martin Roland Oelemann, Fernanda de Avila Abreu, Ana Luisa Andrade-Oliveira, Marcia Giambiagi-deMarval, Marinella Silva Laport

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Abstract

Aims: This study aimed to assess the antimicrobial potential of Bp1-AdE, produced by Bacillus pumilus 64-1, and to investigate its mode of action against Staphylococcus aureus and methicillin-resistant S. aureus (MRSA).

Methods and results: Bp-1AdE, derived from sponge-associated B. pumilus, exhibited bactericidal activity at 1 550 µg ml-1 against S. aureus ATCC29213 and MRSA strains. Light and fluorescence microscopy revealed drastic cell lysis of S. aureus treated with Bp-1AdE. Scanning and transmission electron microscopy suggested that Bp-1AdE disrupts the cytoplasmic membrane. Toxicity assays showed that Bp-1AdE was non-toxic to Tenebrio molitor larvae. Liquid chromatography-mass spectrometry and Global Natural Product Social spectral libraries identified four substances within Bp-1AdE, including aliphatic alcohols [3,4-dipentylhexane-2,5-diol and 1,1'-(4,5-dibutyl-3,6-dimethylcyclohexane-1,2-diyl)bis(ethan-1-one)] and terpenoids (cholic acid and canrenone).

Conclusions: Bp-1AdE demonstrated selective toxicity and bactericidal activity, highlighting its potential for controlling infections caused by multidrug-resistant S. aureus strains.

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海洋枯草芽孢杆菌物质对耐多药金黄色葡萄球菌具有抗菌作用。

目的：本研究旨在评估由枯草杆菌 64-1 产生的 Bp1-AdE 的抗菌潜力，并研究其对金黄色葡萄球菌和耐甲氧西林金黄色葡萄球菌（MRSA）的作用模式：Bp-1AdE 来自海绵相关的布氏杆菌，对金黄色葡萄球菌 ATCC29213 和 MRSA 菌株的杀菌活性为 1,550 µg ml-1。光镜和荧光显微镜检查发现，经 Bp-1AdE 处理的金黄色葡萄球菌的细胞裂解率很高。扫描和透射电子显微镜显示 Bp-1AdE 破坏了细胞质膜。毒性试验表明，Bp-1AdE 对天牛幼虫无毒。液相色谱-质谱（LC-MS）和全球天然产品社会（GNPS）光谱库确定了 Bp-1AdE 中的四种物质，包括脂肪醇[3,4-二戊基己烷-2,5-二醇和 1,1'-(4,5-二丁基-3,6-二甲基环己烷-1,2-二基)双（乙烷-1-酮）]和萜类化合物（胆酸和坎利酮）：结论：Bp-1AdE 具有选择性毒性和杀菌活性，可用于控制耐多药金黄色葡萄球菌菌株引起的感染。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Applied Microbiology 生物-生物工程与应用微生物

CiteScore

7.30

自引率

2.50%

发文量

427

审稿时长

2.7 months

期刊介绍： Journal of & Letters in Applied Microbiology are two of the flagship research journals of the Society for Applied Microbiology (SfAM). For more than 75 years they have been publishing top quality research and reviews in the broad field of applied microbiology. The journals are provided to all SfAM members as well as having a global online readership totalling more than 500,000 downloads per year in more than 200 countries. Submitting authors can expect fast decision and publication times, averaging 33 days to first decision and 34 days from acceptance to online publication. There are no page charges.